Search results for "Pathogenic bacteria"
showing 10 items of 61 documents
Adapting a Phage to Combat Phage Resistance
2020
Phage therapy is becoming a widely recognized alternative for fighting pathogenic bacteria due to increasing antibiotic resistance problems. However, one of the common concerns related to the use of phages is the evolution of bacterial resistance against the phages, putatively disabling the treatment. Experimental adaptation of the phage (phage training) to infect a resistant host has been used to combat this problem. Yet, there is very little information on the trade-offs of phage infectivity and host range. Here we co-cultured a myophage FCV-1 with its host, the fish pathogen Flavobacterium columnare, in lake water and monitored the interaction for a one-month period. Phage resistance was…
Evaluation of Zataria multiflora Boiss. essential oil activity against Escherichia coli O157:H7, Salmonella enterica and Listeria monocytogenes by pr…
2013
Essential oils (EOs) have long been applied as flavoring agents in foods, and due to their content in antimicrobial compounds, they have potential as natural agents for food preservation. Recently, real-time PCR in combination with PMA has successfully been applied to discriminate between live Escherichia coli O157:H7 and dead bacteria killed by cumin, clove, oregano and cinnamon EOs. In this study, initial experiments were performed in order to elucidate the minimum bactericidal concentration of Zataria multiflora EOs on E. coli O157:H7, Salmonella enterica and Listeria monocytogenes. Thereafter PMA-qPCR was applied in order to selectively quantify life cells within a bacterial population …
Preparation and Characterization of Aminoglycoside-Loaded Chitosan/Tripolyphosphate/Alginate Microspheres against E. coli
2021
Although aminoglycosides are one of the common classes of antibiotics that have been widely used for treating infections caused by pathogenic bacteria, the evolution of bacterial resistance mechanisms and their inherent toxicity have diminished their applicability. Biocompatible carrier systems can help sustain and control the delivery of antibacterial compounds while reducing the chances of antibacterial resistance or accumulation in unwanted tissues. In this study, novel chitosan gel beads were synthesized by a double ionic co-crosslinking mechanism. Tripolyphosphate and alginate, a polysaccharide obtained from marine brown algae, were employed as ionic cross-linkers to prepare the chitos…
Antibacterial effects of enniatins J1 and J3 on pathogenic and lactic acid bacteria
2011
Abstract Enniatins (ENs) are N -methylated cyclohexadepsipeptides, secondary metabolites produced by various species of the genus Fusarium . They are known to act as antifungal, antiyeast and antibacterial and to possess antiinsecticidal and phytotoxic properties. In this study we evaluated for the first time the antibiotic effect of pure fractions of EN J 1 and J 3 on several pathogenic strains and lactic acid bacteria. The ENs J 1 and J 3 were purified from the fermentation extract of Fusarium solani growth on solid medium of wheat kamut, using the technique of the low pressure liquid chromatography (LPLC) followed by a semipreparative liquid chromatography (LC). The purity and the struct…
A single method for recovery and concentration of enteric viruses and bacteria from fresh-cut vegetables
2011
Fresh-cut vegetables are prone to be contaminated with foodborne pathogens during growth, harvest, transport and further processing and handling. As most of these products are generally eaten raw or mildly treated, there is an increase in the number of outbreaks caused by viruses and bacteria associated with fresh vegetables. Foodborne pathogens are usually present at very low levels and have to be concentrated (i.e. viruses) or enriched (i.e. bacteria) to enhance their detection. With this aim, a rapid concentration method has been developed for the simultaneous recovery of hepatitis A virus (HAV), norovirus (NV), murine norovirus (MNV) as a surrogate for NV, Escherichia coli O157:H7, List…
In vivo modulation of Hsp70 in Rhynchophorus ferrugineus hemocytes after Bacillus thuringiensis treatment
2010
Heat shock proteins (Hsps) are rapidly synthesized within stressed cells after exposure to an environmental stressor. A variety of environmental stresses, including heat, cold, trace-metal exposure, xenobiotics have been reported to modulate Hsps expression in various organisms. Hsps are grouped into several families based on their protein size. Most organisms have several genes encoding members of this Hsp family. In particularly Hsp70 can be induced quickly under stressful conditions, but return to a normal expression level under non-stressful conditions. Few studies have been done to detect the Hsp70 expression in phytophagous insects towards pathogens. Since a preliminary research discl…
Use of Logistic Regression for Prediction of the Fate of Staphylococcus aureus in Pasteurized Milk in the Presence of Two Lytic Phages
2010
The use of bacteriophages provides an attractive approach to the fight against food-borne pathogenic bacteria, since they can be found in different environments and are unable to infect humans, both characteristics of which support their use as biocontrol agents. Two lytic bacteriophages, vB_SauS-phiIPLA35 (phiIPLA35) and vB_SauS-phiIPLA88 (phiIPLA88), previously isolated from the dairy environment inhibited the growth of Staphylococcus aureus. To facilitate the successful application of both bacteriophages as biocontrol agents, probabilistic models for predicting S. aureus inactivation by the phages in pasteurized milk were developed. A linear logistic regression procedure was used to desc…
Evaluation of dermal toxicity of antibacterial cotton textile coated by sol-gel technology
2017
AbstractThis paper reports about cotton textile modification by sol-gel technology with the purpose of obtaining antibacterial properties, evaluation of antibacterial properties and dermal toxicity tests of cotton textile with Zn and Si coating. Antibacterial properties evaluation against pathogenic microorganisms Pseudomonas aeruginosa, Staphylococcus aureus, and Escherichia coli made using the Parallel streak method in accordance with ATCC147 standard. For more specific evaluation of the coated textile, in vitro cytotoxicity test with epidermal HaCat cells was done. It is concluded that the coatings containing Zn and Si obtained by the sol-gel technology can impart antibacterial propertie…
Occurrence and virulence of Pseudoalteromonas spp. in cultured gilthead sea bream (Sparus aurata L.) and European sea bass (Dicentrarchus labrax L.).…
2007
During two long-term bacteriological surveys in European sea bass (Dicentrarchus labrax L.) and gilthead sea bream (Sparus aurata L.) culture facilities on the Spanish Mediterranean coast, four different species of Pseudoalteromonas were found in the internal organs of animals. Pseudoalteromonas undina, Pseudoalteromonas haloplanktis, P. espejiana and Pseudoalteromonas sp. were isolated from 9% out of 177 European sea bass and 11.7% out of 547 gilthead sea bream and they were recovered as pure cultures in 50% of isolations. Among fish with positive bacterial growth, Pseudoalteromonas was more prevalent in sea bass showing clinical signs (27.7%) and in gilthead sea bream without clinical sig…
Natural soil reservoirs for human pathogenic and fecal indicator bacteria
2015
Prod ? EA UB INRA BIOME; International audience; résumé du livre : Environmental microbiology, the study of the roles that microbes play in all planetary environments, is one of the most important areas of scientific research. The The Manual of Environmental Microbiology, Fourth Edition, provides comprehensive coverage of this critical and growing field. Thoroughly updated and revised, the Manuall is the definitive reference for information on microbes in air, water, and soil and their impact on human health and welfare. Written in accessible, clear prose, the manual covers four broad areas: general methodologies, environmental public health microbiology, microbial ecology, and biodegradati…