Search results for "Patulin"

Detection and quantification of patulin and griseofulvin by high pressure liquid chromatography in different strains of Penicillium griseofulvum Dier…

1988

Patulin and griseofulvin production by twelve strains ofPenicillium griseofulvum Dierckx, eleven of which were isolated from pistachio (Pistacia vera) nuts and the other was supplied by the Spanish Collection of Type Culture, was investigated. Six strains of the eleven isolated had ability to produce patulin and griseofulvin in Yes medium. All the strains studied had no ability to produce patulin in Wickerham medium. Griseofulvin production was significant in both media but higher in Wickerham.These metabolites were separated and determined in the chloroform extracts of cultures by high performance liquid chromatography with ultraviolet detection. The best conditions were: acetonitrile - wa…

Cytotoxic effects of individual and combined mycotoxins, patulin sterigmatocystin and beauvericin on CHO-K1 cells

2015

In vitro cytotoxicity of patulin, deoxynivalenol, nivalenol and zearalenone on CHO-K1 cells

2006

Comparison of different analytical processes for patulin determination in apple juice

2009

F. M. Valle-Algarra, R. Mateo, A. Medina, F. Mateo, E. M. Mateo, E. Sanchis Blanco, J.V. Gimeno-Adelantado, J. Peris-Vicente and M. Jimenez 1 Dpto. de Quimica Analitica, Facultad de Quimica, Universidad de Valencia, Dr. Moliner 50, E-46100 Burjassot, Valencia, Spain 2 Dpto. de Microbiologia y Ecologia, Facultad de Biologia, Universidad de Valencia, Dr. Moliner 50, E-46100 Burjassot, Valencia, Spain 3 Dpto. de Ingenieria Electronica, Universidad Politecnica de Valencia, Camino de Vera 14, E-46022, Valencia, Spain

Differentiation of Penicillium griseofulvum Dierckx isolates by enzyme assays and by patulin and griseofulvin analyses

1990

The production of patulin and griseofulvin by 49 different isolates of Penicillium griseofulvum Dierckx was analyzed by high-performance liquid chromatography. Eleven isolates were obtained from pistachio nuts, 37 were obtained from wheat seeds, and 1 was obtained from the American Type Culture Collection. Activities of 19 enzymes were also assayed by the API ZYM system. From these results it may be deduced that there are two different groups among the strains tested which cannot be distinguished by morphological and cultural characteristics. One group of isolates did not produce detectable amounts of patulin and griseofulvin when grown in sucrose-yeast extract and Wickerham media, while en…

Study of thermal resistance and in vitro bioaccessibility of patulin from artificially contaminated apple products

2012

Abstract Apple juices and purees represent categories widely consumed by whole population and above all children. Patulin (PAT) is a mycotoxin known for its acute and chronic effects in animals. Several studies indicate there is a risk associated to the PAT intake, through the consumption of purees and apple juices. In this study, apple juice and puree were prepared and artificially contaminated with PAT at 50 μg/kg and submitted to a thermal treatment simulating pasteurization to evaluate PAT’s reduction. In a second phase of the work, apple products samples ( n  = 7) included juices, nectars and purees belonging to different commercial brands were collected, artificially contaminated with…

Does low concentration mycotoxin exposure induce toxicity in HepG2 cells through oxidative stress?

2020

The purpose of this study was to determine whether exposure to low concentrations of deoxynivalenol (DON), T-2 toxin (T-2) and patulin (PAT) in a human hepatocellular carcinoma cell line (HepG2) exerts toxic effects through mechanisms related to oxidative stress, and how cells deal with such exposure. Cell viability was determined by the MTT and protein content (PC) assays over 24, 48 and 72 h. The IC

Evaluation of Mycotoxins in Infant Breast Milk and Infant Food, Reviewing the Literature Data

2021

In this review, an analysis focusing on mycotoxin determination in infant breast milk and infant food has been summarised for the last fifteen years of research focused on the intended population group of 1–9 months. The objective was to know the level of exposure of the child population to an estimated daily intake (EDI) of mycotoxins from the consumption of habitual foods. The EDI was compared with the tolerable daily intake (TDI) established by EFSA to estimate risk. In breast milk, the high prevalence and levels were for samples from Africa (Egypt and Tanzania) with aflatoxin M1 (1.9 μg/L and 10%), and Asia (Iran) with ochratoxin-A (7.3 μg/L and 100%). In infant formulas, high incidence…

Producción de anticuerpos y generación de metodologías inmunoquímicas para la detección de biotoxinas

2022

La patulina es un metabolito secundario tóxico producido por diferentes hongos pertenecientes a los géneros Aspergillus, Paecilomyces, Penicillium y Xylaria. Su presencia en la cadena alimentaria se debe a la contaminación de una gran variedad de alimentos con hongos productores, entre los cuales P. expansum, que infecta fundamentalmente manzanas, es considerado el principal hongo productor de patulina. A raíz de su reactividad con compuestos nucleofílicos a nivel celular y sus efectos tóxicos agudos reportados en riñones, intestino y el sistema inmunitario de vertebrados, la patulina se encuentra en la corta lista de micotoxinas vigiladas y reguladas mundialmente. Los límites máximos para …