Search results for "Peas"

Acoso y Derribo

1999

La rebelión ciudadana

2005

¿Leemos el mismo tratado?

2005

Functional analysis of the isoforms of an ABI3-like factor of Pisum sativum generated by alternative splicing

2009

At least seven isoforms (PsABI3-1 to PsABI3-7) of a putative, pea ABI3-like factor, originated by alternative splicing, have been identified after cDNA cloning. A similar variability had previously only been described for monocot genes. The full-length isoform, PsABI3-1, contains the typical N-terminal acidic domains and C-terminal basic subdomains, B1 to B3. Reverse transcriptase-PCR analysis revealed that the gene is expressed just in seeds, starting at middle embryogenesis; no gene products are observed in embryo axes after 18 h post-imbibition although they are more persistent in cotyledons. The activity of the isoforms was studied by yeast one-hybrid assays. When yeast was transformed …

Structural stability and properties of three isoforms of the major light-harvesting chlorophyll a/b complexes of photosystem II.

2008

AbstractThree isoforms of the major light-harvesting chlorophyll (Chl) a/b complexs of photosystem II (LHCIIb) in the pea, namely, Lhcb1, Lhcb2, and Lhcb3, were obtained by overexpression of apoprotein in Escherichia coli and by successfully refolding these isoforms with thylakoid pigments in vitro. The sequences of the protein, pigment stoichiometries, spectroscopic characteristics, thermo- and photostabilities of different isoforms were analysed. Comparison of their spectroscopic properties and structural stabilities revealed that Lhcb3 differed strongly from Lhcb1 and Lhcb2 in both respects. It showed the lowest Qy transition energy, with its reddest absorption about 2 nm red-shifted, an…

Genetic diversity and trait genomic prediction in a pea diversity panel

2014

Background Pea (Pisum sativum L.), a major pulse crop grown for its protein-rich seeds, is an important component of agroecological cropping systems in diverse regions of the world. New breeding challenges imposed by global climate change and new regulations urge pea breeders to undertake more efficient methods of selection and better take advantage of the large genetic diversity present in the Pisum sativum genepool. Diversity studies conducted so far in pea used Simple Sequence Repeat (SSR) and Retrotransposon Based Insertion Polymorphism (RBIP) markers. Recently, SNP marker panels have been developed that will be useful for genetic diversity assessment and marker-assisted selection. Resu…

Compatibility of Rhizobial Genotypes within Natural Populations of Rhizobium leguminosarum Biovar viciae for Nodulation of Host Legumes

2003

ABSTRACT Populations of Rhizobium leguminosarum biovar viciae were sampled from two bulk soils, rhizosphere, and nodules of host legumes, fava bean ( Vicia faba ) and pea ( Pisum sativum ) grown in the same soils. Additional populations nodulating peas, fava beans, and vetches ( Vicia sativa ) grown in other soils and fava bean-nodulating strains from various geographic sites were also analyzed. The rhizobia were characterized by repetitive extragenomic palindromic-PCR fingerprinting and/or PCR-restriction fragment length polymorphism (RFLP) of 16S-23S ribosomal DNA intergenic spacers as markers of the genomic background and PCR-RFLP of a nodulation gene region, nodD , as a marker of the sy…

Ethnic or Socio-Economic Conflict? Political Interpretations of the Rwandan Crisis

1996

AbstractRather than trace the political history of the conflict in Rwanda I will focus on the different interpretations of the conflict by the actors involved. The external identification of the Tutsi refugees as 'Banyarwanda' corresponds with the ideology and self image of the RPF who were recruited among the refugees and their descendants who fled to Uganda during and after 1959. The RPF presents itself as a democratic organisation speaking for all Rwandans and its anti-ethnic stance is designed not only to appeal to Rwandans but also to a Western audience. The RPF's opponent, the Habyarimana government in Rwanda, presented itself as the heir of the 1959 'peasant revolution' which had rep…

Conclusion: Resisting, Cooperating, and Fighting

2016

The three-fold division of this book—how these peasant elites and the peasantry in general confronted the authorities, how they dealt with them, and how they acted within their own local communities and networks—has aimed to place their aggressive and violent behaviour in the framework of Nordic state formation in the sixteenth and seventeenth centuries. The focus has thus been on their relationship with the state and its representatives. This structure contained per se a conflict of interests between states that wanted to intervene and control local communities and the leading peasants in these communities who wanted to guard their favourable positions. At the same time, there were possibi…

Distinct Site Specificity of Two Pea Histone Deacetylase Complexes

2001

We report on the site specificity of two intact pea histone deacetylase complexes. HD1 deacetylates lysines 5 and 16 of H4 in the order K16 > K5, while in the case of H3 the preferred order is K4 >> K18 approximately K9. The specificity of the HD2 complex is markedly different. The preferred residues in H4 are K8 approximately K5 > K16, while in H3 deacetylation, the complex HD2 prefers sites 4 and 18. To obtain these results, we have used a novel procedure based on the SPOT technique, a method to synthesize peptides on membrane supports. Different sets of membranes with sequentially overlapping histone peptides containing acetylated lysines in the sites corresponding to all in vivo acetyla…