Search results for "Phage therapy"
showing 10 items of 40 documents
Counteracting the horizontal spread of bacterial antibiotic resistance with conjugative plasmid-dependent bacteriophages
2016
Microencapsulation of Enteric Bacteriophages in a pH-Responsive Solid Oral Dosage Formulation Using a Scalable Membrane Emulsification Process
2019
A scalable low-shear membrane emulsification process was used to produce microencapsulated Escherichia coli-phages in a solid oral dosage form. Uniform pH-responsive composite microparticles (mean size ~100 µ
Data from: Targeting antibiotic resistant bacteria with phages reduces bacterial density in an insect host
2019
Phage therapy is attracting growing interest among clinicians as antibiotic resistance continues becoming harder to control. However, clinical trials and animal model studies on bacteriophage treatment are still scarce and results on the efficacy vary. Recent research suggests that using traditional antimicrobials in concert with phage could have desirable synergistic effects that hinder the evolution of resistance. Here, we present a novel insect gut model to study phage-antibiotic interaction in a system where antibiotic resistance initially exists in very low frequency and phage specifically targets the resistance bearing cells. We demonstrate that while phage therapy could not reduce th…
Bacteriophage Adherence to Mucus Mediates Preventive Protection against Pathogenic Bacteria
2019
The mucosal surfaces of animals are habitat for microbes, including viruses. Bacteriophages—viruses that infect bacteria—were shown to be able to bind to mucus. This may result in a symbiotic relationship in which phages find bacterial hosts to infect, protecting the mucus-producing animal from bacterial infections in the process. Here, we studied phage binding on mucus and the effect of mucin on phage-bacterium interactions. The significance of our research is in showing that phage adhesion to mucus results in preventive protection against bacterial infections, which will serve as basis for the development of prophylactic phage therapy approaches. Besides, we also reveal that exposure to m…
Isolation of Four Lytic Phages Infecting Klebsiella pneumoniae K22 Clinical Isolates from Spain
2020
This article belongs to the Special Issue Bacteriophage—Molecular Studies.
Fight evolution with evolution: plasmid‐dependent phages with a wide host range prevent the spread of antibiotic resistance
2013
The emergence of pathogenic bacteria resistant to multiple antibiotics is a serious worldwide public health concern. Whenever antibiotics are applied, the genes encoding for antibiotic resistance are selected for within bacterial populations. This has led to the prevalence of conjugative plasmids that carry resistance genes and can transfer themselves between diverse bacterial groups. In this study, we investigated whether it is feasible to attempt to prevent the spread of antibiotic resistances with a lytic bacteriophage, which can replicate in a wide range of gram‐negative bacteria harbouring conjugative drug resistance–conferring plasmids. The counter‐selection against the plasmid was sh…
Antibacterial Efficiency of Surface-Immobilized Flavobacterium-Infecting Bacteriophage
2019
Control of bacterial diseases by bacteriophages (phages) is gaining more interest due to increasing antibiotic resistance. This has led to technologies to attach phages on surfaces to form a biomaterial that can functionally display phages that interact with bacteria, to carry out successful infection cycles. Such a material could be applied in many environments, where the target pathogens are expected. Although this approach has been applied successfully in a few studies already, the basis of the antibacterial effect by the immobilized phages is unclear, and the interpretation of the results depends on the study. Here, we studied the phage attachment density, their detachment rate and infe…
Exploring phage-bacterium interactions : new ways to combat a fish pathogen
2014
Dataset for Mucin induces CRISPR-Cas defense in an opportunistic pathogen
2022
This dataset contains raw data points of (1) phage-bacterium co-evolution experiment (flavobacterium columnare colony counts, phage counts, bacterial growth characteristics and number of CRISPR-Cas spacers) and (2) from competition experiment between Flavobacterium columnare and Aeromonas sp. For detailed method description, see the attached Documentation file.