Search results for "Phage"
showing 10 items of 1573 documents
Purified Membrane-Containing Procapsids of Bacteriophage PRD1 Package the Viral Genome
2009
Icosahedral-tailed double-stranded DNA (dsDNA) bacteriophages and herpesviruses translocate viral DNA into a preformed procapsid in an ATP-driven reaction by a packaging complex that operates at a portal vertex. A similar packaging system operates in the tailless dsDNA phage PRD1 (Tectiviridae family), except that there is an internal membrane vesicle in the procapsid. The unit-length linear dsDNA genome with covalently linked 5'-terminal proteins enters the procapsid through a unique vertex. Two small integral membrane proteins, P20 and P22, provide a conduit for DNA translocation. The packaging machinery also contains the packaging ATPase P9 and the packaging efficiency factor P6. Here we…
Probing protein interactions in the membrane-containing virus PRD1.
2015
PRD1 is a Gram-negative bacteria infecting complex tailless icosahedral virus with an inner membrane. This type virus of the family Tectiviridae contains at least 18 structural protein species, of which several are membrane associated. Vertices of the PRD1 virion consist of complexes recognizing the host cell, except for one special vertex through which the genome is packaged. Despite extensive knowledge of the overall structure of the PRD1 virion and several individual proteins at the atomic level, the locations and interactions of various integral membrane proteins and membrane-associated proteins still remain a mystery. Here, we demonstrated that blue native PAGE can be used to probe pro…
Lactococcal 949 group phages recognize a carbohydrate receptor on the host cell surface
2015
ABSTRACT Lactococcal bacteriophages represent one of the leading causes of dairy fermentation failure and product inconsistencies. A new member of the lactococcal 949 phage group, named WRP3, was isolated from cheese whey from a Sicilian factory in 2011. The genome sequence of this phage was determined, and it constitutes the largest lactococcal phage genome currently known, at 130,008 bp. Detailed bioinformatic analysis of the genomic region encoding the presumed initiator complex and baseplate of WRP3 has aided in the functional assignment of several open reading frames (ORFs), particularly that for the receptor binding protein required for host recognition. Furthermore, we demonstrate th…
Virus-Host Coevolution as a Tool for Controlling Bacterial Resistance to Phage Therapy
2019
Bacterial resistance to antibiotics is a global public health concern. New treatments are needed to combat resistant strains, among which phage therapy is a promising option. Probably the main advantages of phage therapy are its high specificity as well as rapid viral adaptability, which in principle allows using phage evolution to overcome resistance. Here, we have performed serial coevolution passages between Escherichia coli and its phage T7 to investigate the ability of coevolved phages to reduce the emergence of resistances. We find that the initial bacterial population is less likely to undergo resistance when challenged with experimentally coevolved phages than when challenged with t…
Complete Genome Sequence of the Enterobacter cancerogenus Bacteriophage Enc34
2012
ABSTRACT Enterobacter cancerogenus is widely distributed in nature and is generally recovered from environmental or vegetal sources. In some cases, it has also been associated with human infections. In this study, the complete genomic sequence of virulent E. cancerogenus bacteriophage Enc34 was determined. The Enc34 genome is 60,364 bp in length and contains 80 open reading frames. To our knowledge, this is the first report of a bacteriophage infecting E. cancerogenus .
Production of VEGF and b-FGF in the drainage fluid from patients undergoing incisional hernia repair
2005
Wound healing is a complex process involving interaction between different cell types, such as growth factors. Among these, vascular endothelial growth factors (VEGF) and basic fibroblast growth factors (b-FGF) are the most important. The aim of this study was to assess the production of VEGF and b-FGF in wound drainage fluid from patients undergoing incisional abdominal hernia repair. Ten female patients with abdominal midline incisional hernia undergoing surgical repair were included in this study. In all cases a closed suction drain was placed in the wound below the fascia and removed on postoperative day 4. Wound fluid was collected on the I, II, III and IV day and its amount at each ti…
Ligny-le-Châtel (Yonne) : étude typologique et pétrographique des sarcophages
2006
International audience; A la demande conjointe du Service régional de l’archéologie de Bourgogne et de l’INRAP Grand-Est Sud, nous avons eu en charge l’étude typologique et pétrographique des sarcophages de pierre de la nécropole de « La Coupe au Meunier » à Ligny-le-Châtel, fouillée de mai à novembre 2005 sous la direction de Patrick Chopelain (INRAP). Le site a livré 64 sarcophages, ce qui représente 38 % des inhumations inventoriées et fouillées 1. À cela, il conviendrait d’ajouter pour mémoire les exemplaires rencontrés à l’occasion du diagnostic mené en 2001, ainsi que la « cinquantaine de sarcophages découverts entre 1760 et 1825 » signalée dans l’Histoire de la ville de Ligny-le-Chât…
L'étude des sarcophages de l'Yonne
2003
Icauna - L'archéologie dans l'Yonne, Conseil Général de l'Yonne
Les sarcophages de Quarré-les-Tombes (Yonne) : étude typologique et pétrographique
2009
Die Studie der 114 um die Kirche Saint-Georges in Quarré-les-Tombes verteilten Sarkophag-Wannen und Deckel hat ergeben, dass sie typologisch sehr einheitlich sind (es handelt sich hauptsächlich um Exemplare des sogenann- ten bourguignon-champenois-Typs). Auch die petrographischen Untersuchungen bestätigen gewisse Übereinstimmungen. Es liegt nahe, dass die Sarkophage dieser Nekropole aus einem recht beschränkten Produktionsgebiet mit mehreren Werkstätten stammen, die die gleichen genormten Modelle in den gleichen Materialien herstellten.
Role of heat shock protein HSP90 and HSP70 in macrophagic differentiation
2010
Heat shock proteins (HSPs) are molecular chaperones whose expression is increased after many different stresses. They have a protective function helping the cell to cope with lethal conditions. These proteins play an essential role as molecular chaperones by assisting the correct folding of nascent and stress-accumulated misfolded proteins and by preventing their aggregation. My team is interested in understanding the roles of HSPs in two physiological related processes: apoptosis and cell differentiation. The aim of my work is to study the functions of HSP90 and HSP70 in macrophagic differentiation. I first studied the role of HSP90 in macrophagic differentiation. We previously reported th…