Search results for "Phage"

Non-structural proteins P17 and P33 are involved in the assembly of the internal membrane-containing virus PRD1.

2015

AbstractBacteriophage PRD1, which has been studied intensively at the structural and functional levels, still has some gene products with unknown functions and certain aspects of the PRD1 assembly process have remained unsolved. In this study, we demonstrate that the phage-encoded non-structural proteins P17 and P33, either individually or together, complement the defect in a temperature-sensitive GroES mutant of Escherichia coli for host growth and PRD1 propagation. Confocal microscopy of fluorescent fusion proteins revealed co-localisation between P33 and P17 as well as between P33 and the host chaperonin GroEL. A fluorescence recovery after photobleaching assay demonstrated that the diff…

Identification of a ligand on the Wip1 bacteriophage highly specific for a receptor on B. anthracis.

2013

Tectiviridae is a family of tailless bacteriophages with Gram-negative and Gram-positive hosts. The family model PRD1 and its close relatives all infect a broad range of enterobacteria by recognizing a plasmid-encoded conjugal transfer complex as a receptor. In contrast, tectiviruses with Gram-positive hosts are highly specific to only a few hosts within the same bacterial species. The cellular determinants that account for the observed specificity remain unknown. Here we present the genome sequence of Wip1, a tectivirus that infects the pathogen Bacillus anthracis. The Wip1 genome is related to other tectiviruses with Gram-positive hosts, notably, AP50, but displays some interesting differ…

Bakteriofāgu terapijas iespējas

2016

Pārmērīga antibakteriālu līdzekļu lietošana veicina epidēmijas mēroga bakteriālās rezistences pieaugumu attīstītajās un attīstības valstīs. Bakteriālās rezistences pieaugums atdzīvina interesi par alternatīvām antibakteriālām terapijām. Viena no tām iesaista bakteriofāgus – vīrusus, kuriem piemīt spējas inficēt un vairoties baktēriju saimniekšūnās veicinot to lizēšanu. Ir plaši dokumentēta fāgu veikta vairāku rezistentu baktēriju izskaušana, tomēr nav skaidrs, caur kādiem mehānismiem tā tiek panākta. Tas apgrūtina regulējošo iestāžu atļauju izsniegšanu, lai ieviestu bakteriofāgu terapiju klīniskajā praksē. Bakalaura darba mērķis bija noskaidrot veselības aprūpes speciālistu (ārstu) viedokli…

Cooperation between Different CRISPR-Cas Types Enables Adaptation in an RNA-Targeting System

2021

CRISPR-Cas systems are immune systems that protect bacteria and archaea against their viruses, bacteriophages. Immunity is achieved through the acquisition of short DNA fragments from the viral invader’s genome.

Silent rain: does the atmosphere-mediated connectivity between microbiomes influence bacterial evolutionary rates?

2020

ABSTRACT Air carries a vast number of bacteria and viruses over great distances all the time. This leads to continuous introduction of foreign genetic material to local, established microbial communities. In this perspective, I ask whether this silent rain may have a slowing effect on the overall evolutionary rates in the microbial biosphere. Arguably, the greater the genetic divergence between gene ‘donors’ and ‘recipients’, the greater the chance that the gene product has a deleterious epistatic interaction with other gene products in its genetic environment. This is due to the long-term absence of check for mutual compatibility. As such, if an organism is extensively different from other…

Comparison of Delivery Methods in Phage Therapy against Flavobacterium columnare Infections in Rainbow Trout

2021

This dataset consists of three phage therapy experiments to prevent Flavobacterium columnare infections in rainbow trout. Experiments I and II were done in stagnant water, and Experiment III in a flow-through system. See the documentation file attached with the dataset for full description of the methods (also found in the original article).

Yeast Killer Toxin-Like Candidacidal Ab6 Antibodies Elicited through the Manipulation of the Idiotypic Cascade

2014

A mouse anti-anti-anti-idiotypic (Id) IgM monoclonal antibody (mAb K20, Ab4), functionally mimicking a Wyckerhamomyces anomalus (Pichia anomala) killer toxin (KT) characterized by fungicidal activity against yeasts presenting specific cell wall receptors (KTR) mainly constituted by β-1,3-glucan, was produced from animals presenting anti-KT Abs (Ab3) following immunization with a rat IgM anti-Id KT-like mAb (mAb K10, Ab2). MAb K10 was produced by immunization with a KT-neutralizing mAb (mAb KT4, Ab1) bearing the internal image of KTR. MAb K20, likewise mAb K10, proved to be fungicidal in vitro against KT-sensitive Candida albicans cells, an activity neutralized by mAb KT4, and was capable of…

Sicilian pistachio nut extract inhibits inflammatory response in lipopolysaccharide-stimulated macrophages.

2008

Differences in Attachment and Phagocytosis of Salmonella minnesota Strains (S Form, Re Mutant) by Mouse Peritoneal Macrophages: Participation of Endo…

1985

The virulence of bacteria in many phagocytic systems has been studied for several years and it is known that macrophages play an important role in host defence against invading microorganisms and in cooperation with cellular and humoral immune mechanisms. Pathogenic bacteria can be divided into two groups with regard to their fate within phagocytes: (a) extracellular bacteria which are promptly killed after phagocytosis and (b) facultative intracellular bacteria which are resistant to intracellular killing unless macrophages are activated. Humoral immune mechanisms (antibody, complement) deal mainly with extracellular bacteria, while cellular immune mechanisms (T cells, macrophages) deal wi…

Bacteriophage Resistance Affects Flavobacterium columnare Virulence Partly via Mutations in Genes Related to Gliding Motility and Type IX Secretion S…

2020

AbstractIncreasing problems with antibiotic resistance has directed interest towards phages as tools to treat bacterial infections in the aquaculture industry. However, phage resistance evolves rapidly in bacteria posing a challenge for successful phage therapy. To investigate phage resistance in the fish pathogenic bacterium Flavobacterium columnare, two phage-sensitive, virulent wild-type isolates, FCO-F2 and FCO-F9, were exposed to phages and subsequently analyzed for bacterial viability and colony morphology. Twenty-four phage-exposed isolates were further characterized for phage resistance, antibiotic susceptibility, motility, adhesion and biofilm formation on polystyrene surface, prot…