Search results for "Phage"

Impact of biotic and abiotic factors on bacterial virulence

2013

Mandrel-less fabrication of biomimetic electrospun microfiber wires for tissue engineering applications

2021

This study introduces a novel mandrel-less electrodeposition method and a novel electrode prototypes useful for tissue engineering applications. More specifically, in this study a novel fabrication protocol to electrospun fibers-based chords, with length spanning from 2 cm to virtually no limitations on longitudinal length, is presented. For the first part of this study, Poly(ester urethane) urea (PEUU) was used to fabricate, by electrodeposition technique, continuous microfiber wires with controlled micro-architecture and tunable mechanics. A novel apparatus to process degradable or non-degradable polymers was used. Soft tissue injuries are common in daily clinical and surgical practice. O…

Studies on the interaction of C1q,a subcomponent of the first component of complement, with porins fromSalmonella minnesotaincorporated into artifici…

1990

AbstractPurified outer membrane proteins (OMP) of Salmonella minnesota, Re-form, were incorporated into liposomes. These induced in macrophages a chemiluminescence signal identical to that of the intact Re-form. This signal was abolished by preincubation of porin-containing liposomes with purified C1q. Incorporation of isolated OMP into black lipid membranes (BLM) resulted in channel-formation which could not be inhibited by isolated C1q. Additionally, incubation of OMP-containing liposomes with BLM resulted in pore-formation within the BLM. This was amplified when lipid A was present within the liposomes. Preincubation of OMP-containing liposomes with purified C1q abolished pore-formation …

Bayesian Analysis of Diagnostic Accuracy for Gastroesophageal Reflux Disease in the absence of gold standard

2007

Bakteriofagi: nova paradigma ta perevagi  pered antibiotikami u likuval'no-profilakticnih  ŝljah

2017

Comparative analysis of antibiotics and bacteriophages preparations properties. Substantiation of prospects of using bacteriophages in medical purposes. Methods: systematic analysis, analytical, monographic, synthesis, formalization, abstract and logical. Many years of antibiotics use for the treatment of various diseases has led to the emergence of multidrug-resistant bacterial strains. According to WHO, more than 60% of pathogens are resistant to major antibiotics, and in 10-20 years almost all will acquire antimicrobial resistance. The reasons for the rapid adaptation of microorganisms to antibiotics may include continual, unreasonable using of antibiotics for self-medication, and when t…

Targeting antibiotic resistant bacteria with phages reduces bacterial density in an insect host

2018

Phage therapy is attracting growing interest among clinicians as antibiotic resistance continues becoming harder to control. However, clinical trials and animal model studies on bacteriophage treatment are still scarce and results on the efficacy vary. Recent research suggests that using traditional antimicrobials in concert with phage could have desirable synergistic effects that hinder the evolution of resistance. Here, we present a novel insect gut model to study phage-antibiotic interaction in a system where antibiotic resistance initially exists in very low frequency and phage specifically targets the resistance bearing cells. We demonstrate that while phage therapy could not reduce th…

Locust neuropeptides stimulating sex pheromone production in female European corn borer moth, Ostrinia nubilalis

1990

Abstract The presence of a pheromonotropically active hormone (PBAN-like substance) from locust organs, ( Locusta migratoria ) has been demonstrated. Extracts of brain, corpora cardiaca, corpora allata, suboesophageal ganglia and thoracic ganglia of L. migratoria caused highly pheromonotropic responses in decapitated females of the European corn borer moth ( Ostrinia nubilalis ). The highest activity per unit weight of tissue was found in the corpora cardiaca and corpora allata of the locust followed by the brain plus suboesophageal ganglion of O. nubilalis . Following 8 days of disconnection, separating the corpora allata from the corpora cardiaca and suboesophageal ganglion, the pheromono…

Mechanisms involved in lipid accumulation and apoptosis induced by 1-nitropyrene in Hepa1c1c7 cells

2011

International audience; 1-Nitropyrene (1-NP) is a nitro-polycyclic aromatic hydrocarbon (nitro-PAH) present in diesel exhaust and bound to particular matter in urban air. We show that 1-NP and the referent PAH benzo(a)pyrene (BP) induce apoptosis and a lipid accumulation dependent on cytochrome P450 1A1-metabolites in mouse hepatoma cells, whereas 1-amino-pyrene had no effect. The caspase inhibitor, N-benzyloxycarbonyl-Val-Ala-Asp(O-Me) fluoromethyl ketone (Z-VAD-fmk), inhibits 1-NP-induced apoptosis, but failed to alter 1-NP-triggered lipid accumulation determined by Nile red staining. We further show that cholesterol and fatty acid contents are modified after nitro-PAH exposure and that 1…

Biocontrol of the Major Plant Pathogen

2019

Three new lytic bacteriophages were found to effectively control the pathogen Ralstonia solanacearum, a quarantine bacterium in many countries, and causative agent of bacterial wilt, one of the most important vascular plant diseases. Bacterial wilt management has been carried out with fluctuating effects, suggesting the need to find alternative treatments. In this work, three lytic phages were isolated from environmental water from geographically distant regions in Spain. They proved to specifically infect a collection of R. solanacearum strains, and some of the closely related pathogenic species Ralstonia pseudosolanacearum, without affecting non-target environmental bacteria, and were abl…

Myotomy of cricopharungeal muscle in the therapy of dyskinesia of upper esophageal sphincter: personal experience

2012

Dyskinesia of the esophagus includes the entire esophagus from the upper esophageal sphincter to the lower esophageal sphinc - ter together with a wide spectrum of physi - opathological and clinical variations. Related to functional diseases such as Dyskinesia of UES (Upper Esophageal Sphincter), myo- tomy is an excellent procedure for patients with cervical dysphagia. In our experience, the surgical treatment is the only procedure to treat UES dyskinesia. For a successful in - tervention is mandatory: a pharyngeal pump efficiency, hypertension of UES and conservation of esophageal peristalsis in the absence of GERD (Gatroesophageal Reflux Disease). From January 2006 to December 2011, the A…