Search results for "Phaseolus"
showing 10 items of 27 documents
Intracellular localisation of some peptidases and alpha-mannosidase in cotyledons of resting kidney bean, Phaseolus vulgaris
1986
Cotyledons of resting kidney beans (Phaseolus vulgaris, L., cv. “Processor”) contain high activities of two alkaline peptidases, an aminopeptidase (EC 3.4.11) acting on Leu-Tyr and Leu-Gly-Gly and a dipeptidase (EC 3.4.13) hydrolysing Ala-Gly together with low activities of neutral naphthyiamidases (marker substrate Leu-β-NA) and of acid carboxypeptidases (EC 3.4.16; marker substrate Z-Phe-Ala). The intracellular localisation of these peptidases and that of α-mannosidase (EC 3.2.1.24) was studied by subcellular fractionations in different media. In density gradient centrifugations in non-aqueous glycerol-potassium iodide media the alkaline peptidases remained mainly in the application zone …
Effect of regurgitant from Leptinotarsa decemlineata on wound responses in Solanum tuberosum and Phaseolus vulgaris.
2002
The effect of regurgitant from Leptinotarsa decemlineata Say larvae on wound-induced responses was studied using two plant species, Solanum tuberosum L. and Phaseolus vulgaris L. Wounding of one leaf of intact S. tuberosum plants differentially affected ethylene production and activities of peroxidase and polyphenol oxidase. Only polyphenol oxidase activity was stimulated by wounding in both wounded and systemic leaves. Peroxidase activity was not affected by wounding. Wounding caused only a transient increase of ethylene production from wounded leaves. The application of regurgitant to wound surfaces stimulated ethylene production as well as activities of peroxidase and polyphenol oxidase …
Local response of bacterial densities and enzyme activities to elevated atmospheric CO2 and different N supply in the rhizosphere of Phaseolus vulgar…
2008
Publication Inra prise en compte dans l'analyse bibliométrique des publications scientifiques mondiales sur les Fruits, les Légumes et la Pomme de terre. Période 2000-2012. http://prodinra.inra.fr/record/256699; Altered flux of labile C from plant roots into soil is thought to influence growth and maintenance of microbial communities under elevated atmospheric CO2 concentrations. We studied the abundance and function of the soil microbial community at two levels of spatial resolution to assess the response of microorganisms in the rhizosphere of the whole root system and of apical root zones of Phaseolus vulgaris L. to elevated CO2 and high or low N supply. At the coarser resolution, microb…
Impact of heteroptera pentatomidae feeding and oviposition on photosynthesis of herbaceous plants and egg parasitoids host location
2009
The present study was designed to examine the effect of heteroptera pentatomidae feeding and/or oviposition on photosynthesis. Murgantia histrionica and Nezara viridula were fed on Brassica and Phaseolus plants, respectively. The changes in photosynthetic gas exchange and chlorophyll fluorescence parameters were followed immediately after the feeding as well as during the post-feeding period (24, 48 and 72 h). Murgantia feeding caused visible damage on leaf lamina, whereas this was not the case with Nezara feeding. In both cases a substantial decrease (50%) in photosynthesis was observed, which was partially restored only in Phaseolus plants by the end of the experiment. By using high resol…
Calcium, iron and zinc uptakes by Caco-2 cells from white beans and effect of cooking
2006
White beans (Phaseolus vulgaris L.) have an interesting content of essential elements, calcium, iron and zinc, but they content also phytates, oxalates, proteins, polyyphenols and complex polysaccharides that are known to interact with minerals and to affect their bioavailability. The bioavailability of calcium, iron and zinc from raw and cooked white beans was estimated using their uptake by Caco-2 cells as the criteria. Previously, the mineral fraction (soluble or dialysable) to be added to the Caco-2 cell monolayer was selected. The results obtained show that cooking increases the Caco-2 cells' uptake percentages (calcium, 18.8 versus 3.6; iron, 33.7 versus 1.7; and zinc, 17.2 versus 2.1…
A portable NMR sensor to measure dynamic changes in the amount of water in living stems or fruit and its potential to measure sap flow.
2014
Nuclear magnetic resonance (NMR) and NMR imaging (magnetic resonance imaging) offer the possibility to quantitatively and non-invasively measure the presence and movement of water. Unfortunately, traditional NMR hardware is expensive, poorly suited for plants, and because of its bulk and complexity, not suitable for use in the field. But does it need to be? We here explore how novel, small-scale portable NMR devices can be used as a flow sensor to directly measure xylem sap flow in a poplar tree (Populus nigra L.), or in a dendrometer-like fashion to measure dynamic changes in the absolute water content of fruit or stems. For the latter purpose we monitored the diurnal pattern of growth, ex…
Reactive oxygen and ethylene are involved in the regulation of regurgitant-induced responses in bean plants.
2004
Summary Application of regurgitant from Leptinotarsa decemlineata Say on wound surfaces of one wounded leaf of intact bean ( Phaseolus vulgaris L.) plants resulted in activation of ethylene biosynthesis followed by an increase of both peroxidase and polyphenol oxidase activity. The aim of the present investigation was to study the source of increased oxidative enzyme activities in regurgitant-treated bean leaves and to determine if hydrogen peroxide and ethylene biosynthesis is responsible for regurgitant-induced amplification of wound responses in bean plants. As the regurgitant contained relative high activities of both peroxidase and polyphenol oxidase, there is a possibility that increa…
Spatially variable rate herbicide application on durum wheat in Sicily
2004
Using the conventional farming system, durum wheat requires high rates of herbicide spraying. Herbicide residues can cause pollution of soil and ground water and, therefore, of the entire environment. In order to minimise the environmental impact of herbicides, a home made system for spatially variable rate crop input application was designed, developed and set up by the Department of Engineering and Technologies in Agriculture and Forestry (I.T.A.F.). This system consists of a DGPS, a portable computer, a specifically developed software and a device for applying rates proportionally related to the machine forward speed (DPA). Tests of spatially variable rate herbicide application were carr…
Effect of thermal treatments on vitality and physical characteristics of bean, chickpea and lentil
2012
Thermal disinfestation treatments are relatively easy to apply, leave no chemical residues and may have some fungicidal activity. However, temperature and time combinations required to kill insect pests may meet or exceed those that reduce the viability of seeds, nutrients content, shelf life or technological characteristics. The aim of this study was to investigate the effect of thermal treatments (different temperature and time combinations) on physical and biological characteristics of bean, chickpea and lentil. Seed samples of common bean, chickpea and lentil were treated at low (12, 24 or 48 h at −18 °C) or high (30, 60 or 90 min at 60 °C) temperature. Seed germination, mean germinatio…
Development of Sicilian bean core collection using morphological descriptors
2018
Different species and varieties of bean, spread in Sicily, are representative of local agricultural practices, as result of a careful exploration. Many landraces have become obsolete due to the spread of commercial varieties, but are still cultivated in small areas of Nebrodi Mountains (ME-Italy) and are endangered. The Sicilian bean landraces are often poorly known but represent a genetic heritage to be preserve and to enhance. The ex situ conservation of Sicilian bean landraces was carried out in “Living Plants Germplasm Bank” of Ucria (ME-Italy), founded by the Nebrodi Regional Park, and in “Sicilian Plant Germplasm Repository” of STEBICEF Department - University of Palermo. Within ex si…