Search results for "Phenotyping"
showing 10 items of 197 documents
In the context of sustainable agriculture how, with plant high throughput phenotyping, can we address the various challenges?
2018
International audience; In a context of climate change and soil and water resource degradation, it becomes increasingly important to reduce the need for high nutrient, water, or pesticides inputs, leading to more sustainable agricultural practices. In this context, our aim is to select the best performing crops in various deleterious abiotic environments, having both a higher yield and a better quality, a better environmental « efficiency ». Because plants interact with numerous and diverse microorganisms, especially in the soil volume surrounding roots, called rhizosphere, plant-microorganism relationships in the rhizosphere is also of great agronomical and ecological importance. To addres…
Does the memory of a first water deficit enable a more efficient response to a subsequent water deficit?
2021
National audience; In the current context of climate change, periods of water deficit occur more frequentlyalong the crop cycle, leading to high yield losses. To limit the negative impact of recurrentwater deficits, plants can adapt, via the mobilization of “stress memory”, allowing them torespond to a subsequent stress in a faster and/or more intensive manner. After a first stressevent, plants can keep an imprint of this stress via the induction of epigenetic (e.g. memorygene regulation), physiological (e.g. stomatal closure) and molecular (e.g. compoundaccumulation) changes. When maintained between two stress periods, these changes mayprepare plants for a subsequent water deficit.This wor…
A genetic and molecular approach to identify transcription factors controlling maize root adaptive response to water deficit
2022
Water stress is recognized as the most severe abiotic stress for agricultural productivity. Root traits play a key role in tolerance to water stress but have largely been neglected in selection schemes. In order to identify the maize genetic bases of the root adaptive responses to water deficit (WD), we used a MAGIC mapping population of 400 lines based on the intercrossing of 16 genotypes. The fine phenotyping of the different genotypes was performed under contrasting water supply on the French root phenotyping platform (4PMI). On the 16 founder genotypes, in addition of phenotyping, we sampled different root tissues daily over 7 days after irrigation arrest and performed RNAseq. On the ba…
Immunogenic hotspots in the spacer domain of ADAMTS13 in immune‐mediated thrombotic thrombocytopenic purpura
2021
International audience; Background Immune-mediated thrombotic thrombocytopenic purpura (iTTP) is caused by anti-ADAMTS13 autoantibodies inducing a severe deficiency of ADAMTS13. Epitope mapping studies on samples obtained during acute iTTP episodes have shown that the iTTP immune response is polyclonal, with almost all patients having autoantibodies targeting the spacer domain of ADAMTS13.Objectives To identify the immunogenic hotspots in the spacer domain of ADAMTS13.Patients/methods A library of 11 full-length ADAMTS13 spacer hybrids was created in which amino acid regions of the spacer domain of ADAMTS13 were exchanged by the corresponding region of the spacer domain of ADAMTS1. Next, th…
Instruction of haematopoietic lineage choices, evolution of transcriptional landscapes and cancer stem cell hierarchies derived from an AML1-ETO mous…
2013
The t(8;21) chromosomal translocation activates aberrant expression of the AML1-ETO (AE) fusion protein and is commonly associated with core binding factor acute myeloid leukaemia (CBF AML). Combining a conditional mouse model that closely resembles the slow evolution and the mosaic AE expression pattern of human t(8;21) CBF AML with global transcriptome sequencing, we find that disease progression was characterized by two principal pathogenic mechanisms. Initially, AE expression modified the lineage potential of haematopoietic stem cells (HSCs), resulting in the selective expansion of the myeloid compartment at the expense of normal erythro- and lymphopoiesis. This lineage skewing was foll…
An Adapted Gating Strategy Integrating a Myelomonocytic Window Is Necessary For Correct Flow Cytometric Diagnosis In a Large Proportion Of AML With M…
2013
Abstract Background Previously, it has been reported, that AML with mutated NPM1 is associated with a distinctive immunophenotype. In particular, low or absent expression of CD34 accompanied by high expression of CD33, and – at least in part of the cases - absence of HLA-DR expression was reported. CD45/side scatter (SSC) gating is widely used for the identification of blasts by flow cytometry (FC). Blast cell gates typically are defined by a low SSC and moderate CD45 expression. However, in a number of patients with NPM1mutation this typical blast cell gate comprises significantly lower blast percentages when compared to the morphological evaluation. In these patient samples a second popul…
Integrated longitudinal immunophenotypic, transcriptional, and repertoire analyses delineate immune responses in patients with COVID-19
2021
To understand how a protective immune response against SARS-CoV-2 develops over time, we integrated phenotypic, transcriptional and repertoire analyses on PBMCs from mild and severe COVID-19 patients during and after infection, and compared them to healthy donors (HD). A type I IFN-response signature marked all the immune populations from severe patients during the infection. Humoral immunity was dominated by IgG production primarily against the RBD and N proteins, with neutralizing antibody titers increasing post infection and with disease severity. Memory B cells, including an atypical FCRL5+ T-BET+ memory subset, increased during the infection, especially in patients with mild disease. A…
The prognostic and predictive value of IKZF1 and IKZF3 expression in T-cells in patients with multiple myeloma
2018
Purpose: While recent studies described the role of IKZF1/3 proteins in multiple myeloma (MM) cells, few have highlighted the significance of IKZF1/3 expression in T-cells. In this study we examine the prognostic and predictive value of IKZF1/3 expression in T-cells in patients with MM stage III. Experimental design: We analysed the IKZF1/3 expression levels in T-cells from 45 MM stage I (MMI) and 50 newly diagnosed MM stage III (MMIII) patients, according to Durie-Salmon staging system, by flow cytometry to examine their prognostic and predictive value. We also combined in vivo observations with in vitro assays to determine the effect of IKZF1/3 expression on the T-cell immunophenotype and…
B lymphocyte intestinal homing in inflammatory bowel disease.
2011
Abstract Background Inflammatory bowel disease (IBD) is thought to be due to an abnormal interaction between the host immune system and commensal microflora. Within the intestinal immune system, B cells produce physiologically natural antibodies but pathologically atypical anti-neutrophil antibodies (xANCAs) are frequently observed in patients with IBD. The objective is to investigate the localisation of immunoglobulin-producing cells (IPCs) in samples of inflamed intestinal tissue taken from patients with IBD, and their possible relationship with clinical features. Methods The IPCs in small intestinal, colonic and rectal biopsy specimens of patients with IBD were analysed by means of immun…
Bone marrow biopsy in Hodgkin's lymphoma
2004
In the study of patients with Hodgkin's lymphoma (HL) the evaluation of bone marrow biopsy (BMB) can be difficult. In this review we analyze the main diagnostic features and the clinical risk factors of BM involvement. Although the role of BMB is criticized by some authors, its value is irreplaceable in the staging of HL and in the diagnosis of primary medullary HL. The Ann Arbor staging committee criteria should be revised and updated in the light of the current immunohistochemical studies that give a fundamental help in the diagnostic process. A single BMB should be adequate for diagnosis in most instances. In cases of suspicious involvement a controlateral BMB could be performed.