Search results for "Phosphodiester bond"

showing 2 items of 12 documents

Identification of the reaction products of (2'-5')oligoadenylate synthetase in the marine sponge.

1998

Previously we reported on the presence of a high (2'-5')oligoadenylate synthetase activity in the marine sponge Geodia cydonium [Kuusksalu, A., Pihlak, A., Muller, W. E. G. & Kelve, M. (1995) Eur. J. Biochem. 232, 351-357]. The presence of (2'-5')oligoadenylates [(2'-5')A] in crude sponge extract was shown by radioimmunoassay and by their HPLC comigration with authentic (2'-5')A oligomers. In addition, the sponge (2'-5')oligoadenylates displayed biological activity, as determined by inhibition studies of protein biosynthesis in rabbit reticulocyte lysate. In the present study individual (2'-5')oligoadenylates synthesized by sponge enzyme were separated by HPLC. The exact composition of ever…

chemistry.chemical_classificationMagnetic Resonance Spectroscopy2'-5'-OligoadenylateOligonucleotideBiological activityNuclear magnetic resonance spectroscopyBiologyBiochemistryPoriferaEnzymemedicine.anatomical_structureBiochemistrychemistryReticulocyteSpectrometry Mass Matrix-Assisted Laser Desorption-IonizationPhosphodiester bondProtein biosynthesismedicine2'5'-Oligoadenylate SynthetaseAnimalsChromatography High Pressure LiquidEuropean journal of biochemistry
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Microstructure analysis of biocompatible phosphoester copolymers

2013

Copolymers with varying compositions of 2-ethoxy-2-oxo-1,3,2-dioxaphospholane (EEP) and 2-ethoxy-4-methyl-2-oxo-1,3,2-dioxaphospholane (EMEP) have been synthesized via 1,5,7-triazabicyclo[4.4.0]dec-5-ene-catalyzed anionic ring-opening polymerization. The molecular weights and comonomer ratios were well controlled and polymers with reasonable molecular weight distributions (<1.5) were obtained in all cases. The copolymers were investigated by 1H and 31P NMR spectroscopies to determine the underlying microstructure via detailed dyad analysis. The copolymers were found to be nontoxic to HeLa cells. Furthermore, the obtained copolymers of EEP and EMEP show thermoresponsive properties, i.e., exh…

chemistry.chemical_classificationPolymers and PlasticsMolecular massComonomerOrganic ChemistryBioengineeringPolymerMicrostructureBiochemistryLower critical solution temperaturechemistry.chemical_compoundPolymerizationchemistryPolymer chemistryPhosphodiester bondCopolymerPolymer Chemistry
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