Search results for "Photosynthesis"
showing 10 items of 304 documents
Modulation of Fatty Acid Patterns During Protoplast Isolation
1983
Protoplast isolation and regeneration is known from numerous plant species (Binding et al. 1). But there are plant species which show very unconstant regeneration or no reaction at all, even if the isolation showed high viability of the protoplasts. Does this lacking response only depend on medium composition and effectors or are there other unknown conditions? The procedure of protoplast isolation and the use of strong hypertonic media is a dramatic disturbance of cell metabolism and might be a stress situation, which cannot be overcome by the cell. Fleck et al. (2) reported “osmotic-stress proteins” in Nicotiana sylvestris protoplasts and Kaiser et al. (3) showed a remarkable influence of…
The Influence of (2-Chloroethyl)trimethylammoniumchloride (CCC) on Growth and Photosynthetic Metabolism of Young Wheat Plants (Triticum aestivum L.)
1984
Summary The influence of (2-chloroethyl)trimethylammoniumchloride on growth and cell metabolism of Triticum aestivum var. Kolibri was investigated. CCC was added to the nutrient solution on the 11th day of development with a final concentration of 10 −2 mol·1 −1 . It immediately stopped root growth and showed a strong inhibitory influence on the growth of the leaves. Smaller and fewer cells accounted for the reduction in final leaf size of about 40 %. The content of soluble proteins and soluble reducing sugars were significantly increased in the treated leaves. CCC also exhibited a stimulating effect on the synthesis of RubPc-ase protein and the in vitro activity of this enzyme. In contrast…
Carotenoids and the Assembly of Light-harvesting Complexes
2006
Carotenoids are constitutive components of all light-harvesting complexes in plants and many such complexes in bacteria. In the crystal structures of several light-harvesting complexes, carotenoids are seen to span the lipid bilayer and connect components of the complex on both membrane surfaces and/or to mediate the interaction of transmembrane protein helices. This important stabilizing function suggests that these pigments are also actively involved in the assembly of light-harvesting complexes. Verification of this notion appears too ambitious a goal at present, as the question of how the pigment-protein complexes of the photosynthetic apparatus are assembled is still open. However, inf…
Inhibitory Action of Glufosinate on Photosynthesis
1993
Glufosinate (phosphinothricin) irreversibly blocks the glutamine synthetase which subsequently gives rise to an accumulation of ammonium and to a strong decrease in some amino acids, especially glutamine and glutamate. Under atmospheric conditions (400 ppm CO2, 21% O2) glufosinate causes a rapid inhibition of photosynthesis, too. H ow ever, under non-photo respiratory conditions (1000 ppm CO2, 2% O2) only a slight inhibition of photosynthesis occurs with glufosinate. Since under both conditions an accumulation of ammonium occurs, it is concluded that inhibition of photosynthesis is not induced by the higher concentrations of ammonium. The results rather suggest that the absence of amino don…
The Effect of Light on the Growth of Pea Plants and the Subsequent Influence in Shikimate Oxidoreductase (EC 1.1.1.25) Activity
1981
Summary Pea plants were cultured in white light, red and far red light, and in the dark during a period of three weeks. At several states of development we investigated the activity of the enzyme shikimate oxidoreductase, the amount of fresh and dry matter, and the contents of protein in stem, leaves, cotyledons, and roots. The enzyme activity was found to be distributed organ-specifically and uninfluenced by the phytochrome system, but it was significantly depressed in plants grown in the dark compared to plants grown in white light. Enzyme activity occurred also in non photosynthetic plants. Regarding the different light conditions the activity of shikimate oxidoreductase was found to cor…
Cardenolides of Digitalis obscura: The effect of phosphate and manganese on growth and productivity of shoot-tip cultures
1997
Abstract Cardenolide composition of leaves from wild and micropropagated elite plants of Digitalis obscura (genotype T4) has been investigated and no qualitative differences were found among their major cardenolides (series A). All of the detected glycosides belong to the digitoxose-type cardenolides. Genins represented less than 2% of the overall content, while lanatoside A was the predominant cardenolide ( ca. 65%) in all samples. The cardenolide yield of micropropagated D. obscura plants depended on the age and development of the cultures, but productivity of long-term cultures (2 years) was quite similar to that of the parent plant. Changes in the concentrations of phosphate or manganes…
The Effect of Light Intensity During Growth of Sinapis alba on the Electron-Transport and the Noncyclic Photophosphorylation
1975
The photosynthetic rate of Sinapis alba can be modified over a wide range by the light intensity during growth. Our present results indicate that there exist regulatory mechanisms in the field of photosynthetic primary reactions. We compared the effect of different light intensities during growth of Sinapis plants on the concentrations of soluble proteins, manganese and lipophilic plastid quinones, the electron flow from water to ferricyanide and noncyclic phosphorylation. We further determined the light dependence curves for the uncoupled electron transport with ferricyanide as electron acceptor and methylammonium-chloride as an uncoupler of photophosphorylation.
The Effect of Bialaphos on Ammonium-Assimilation and Photosynthesis I. Effect on the Enzymes of Ammonium-Assimilation
1989
Abstract In this investigation, the effect of bialaphos (phosphinothricyl-alanyl-alanine) on the enzymes involved in NH4 +-assimilation - glutamine synthetase, glutamine-2-oxoglutarate aminotransferase, glutamate dehydrogenase - is examined and compared to the effect of phosphinothricin (glufosinate) on the same enzymes. Bialaphos was given to whole plants (in vivo) and to leaf homogenate (in vitro). The investigation showed that bialaphos has an inhibiting effect on glutamine synthetase in vivo, but not in vitro. In contrast to this, phosphinothricin inhibits glutamine synthetase in vitro as well as in vivo. It was found that bialaphos, similar to phosphinothricin, does not inhibit glutami…
The Effect of Bialaphos on Ammonium-Assimilation and Photosynthesis II. Effect on Photosynthesis and Photorespiration
1989
Abstract The application of bialaphos (phosphinothricyl-alanyl-alanine) effects a quick photosynthesis inhibition under atmospheric conditions (400 ppm CO2, 21% O2). However, under conditions (1000 ppm CO2, 2% O2) under which photorespiration cannot occur there is no photosynthesis inhibition. In the previous investigation it could be shown that bialaphos splits in plants into phosphinothricin and alanine. The inhibition of glutamine synthetase through freed phosphinothricin results in an NH4 +-accumulation and a decrease in glutamine. With the addition of glutamine, photosynthesis inhibition by bialaphos can be reduced. An NH4 +-accumulation takes place under atmospheric conditions as well…
Correlative Analysis of the Photosynthetic Capacity and Different Components of the Photosynthetic Apparatus
1984
The majority of higher plants is able to adapt to the ecological factor light in a wide range. Depending on the light intensity and the light quality during growth, plant with an equal genotype develop into so-called low light and high light forms. The photosynthetic adaptation to different light conditions involves complex, balanced changes of many leaf features. The changes of physiological factors of photosynthesis includes differences in the CO2 conductance, in the Calvin cycle enzymes, the capacity of electron transport, the photophosphorylation and the pigments (Boardman, 1977; Wild, 1979; Bjorkman, 1981; Lichtenthaler et al., 1981). The adaptation of individual plants or leaves to lo…