Search results for "Phycocyanin"
showing 10 items of 11 documents
Fresh water, marine and terrestrial cyanobacteria display distinct allergen characteristics.
2017
During the last decades, global cyanobacteria biomass increased due to climate change as well as industrial usage for production of biofuels and food supplements. Thus, there is a need for thorough characterization of their potential health risks, including allergenicity. We therefore aimed to identify and characterize similarities in allergenic potential of cyanobacteria originating from the major ecological environments. Different cyanobacterial taxa were tested for immunoreactivity with IgE from allergic donors and non-allergic controls using immunoblot and ELISA. Moreover, mediator release from human FceR1-transfected rat basophilic leukemia (RBL) cells was measured, allowing in situ ex…
Advancing cyanobacteria biomass estimation from hyperspectral observations: Demonstrations with HICO and PRISMA imagery
2021
Retrieval of the phycocyanin concentration (PC), a characteristic pigment of, and proxy for, cyanobacteria biomass, from hyperspectral satellite remote sensing measurements is challenging due to uncertainties in the remote sensing reflectance (?R) resulting from atmospheric correction and instrument radiometric noise. Although several individual algorithms have been proven to capture local variations in cyanobacteria biomass in specific regions, their performance has not been assessed on hyperspectral images from satellite sensors. Our work leverages a machine-learning model, Mixture Density Networks (MDNs), trained on a large (N = 939) dataset of collocated in situ chlorophyll-a concentrat…
Plankton participation in the performance of three constructed wetlands within a Mediterranean natural park.
2019
Abstract The contribution of plankton in the performance of three constructed wetlands (CWs) within the Albufera de Valencia Natural Park has been analyzed, taxonomic group by group, over a two-year operation period in the different sectors of each CW: horizontal subsurface-flow –A–, free-water surface flow –B– and lagoons –C–. Tancat de la Pipa CW (TPCW) only contains B–C sectors, while the others have all three types. Treatment efficiency of each sector type on the taxonomic groups was evaluated by calculating frequency of phytoplankton reduction (or zooplankton production), mass removal (or production) efficiencies and rates, and accumulated removed phytoplankton mass (or produced zoopla…
In vivo and in vitro antioxidant properties of furosemide
2003
The aim of this study was to investigate in vivo and in vitro antioxidant properties of furosemide. In vitro, human red blood cells were submitted to oxidative stress (AAPH), in absence or in presence of different concentrations of furosemide. Potassium efflux was measured in order to quantify the oxidative stress after the action of AAPH on red blood cells. Allophycocyanin assay was also used to investigate antioxidant capacities of furosemide. For the in vivo experiment, male Wistar rats were used. A control group (n = 5) was treated by a daily intraperitoneal injection of saline solution (0.2 ml); 2 other groups (J0 and J+) were treated for 7 days by one daily intraperitoneal injection o…
Phycocyanobilin in solution – a solvent triggered molecular switch
2014
We present a computational investigation of the conformational response of phycocyanobilin (PCB) to the ability of solvents to form hydrogen bonds. PCB is the chromophore of several proteins in light harvesting complexes. We determine the conformational distributions in different solvents (methanol and hexamethylphosphoramide HMPT) by means of ab initio molecular dynamics simulations and characterize them via ab initio calculations of NMR chemical shift patterns. The computed trajectories and spectroscopic fingerprints illustrate that the energy landscape is very complex and exhibits various conformations of similar energy. We elucidate the strong influence of the solvent characteristics on…
Chitosomes as drug delivery systems for C-phycocyanin: preparation and characterization.
2010
The aim of this work was to investigate chitosomes, i.e. liposomes coated by a polyelectrolyte complex between chitosan (CH) and xantan gum (XG), as potential delivery system for oral administration of the protein C-phycocyanin. To this purpose several CH-XG-microcomplexes were prepared in aqueous lactic acid at different chitosan-xanthan gum percent ratios and rheological properties of the microcomplexes were studied to analyse the contribution of chitosan and xanthan gum in the reaction of microcomplexation. After establishing the best microcomplexes, chitosomes were prepared by coating C-phycocyanin loaded liposomes with the CH-XG hydrogels using spray-drying or freeze-drying. The chitos…
Protein c-phycocyanin, structure, physicochemical and biological properties, methods of extraction
2022
С-phycocyanin (phycocyanin) is a pigment-protein complex of the lightharvesting phycobiliprotein family that takes part in the primary phase of photosynthesis in lower plants. The phycocyanin content depends on such factors as the species of microalgae, physical state of biomass, extraction techniques, etc. The main methods for obtaining phycocyanin from biomass include chemical, physical and enzyme treatments with the following purification by such methods as precipitation with ammonium sulfate, ion exchange chromatography, and gel filtration chromatography. The commercial value of phycocyanin is directly related to the methods of its obtaining, purification and purity. The ratio of absorb…
KOSMOS 2017 Peru Side Experiment: nutrients, phytoplankton abundances, enzyme rates, photophysiology
2022
This data was collected during an short-term incubation experiment in March 2017 that investigated the response of a surface plankton community to upwelling. This experiment was carried in the framework of the SFB754-funded KOSMOS mesocosm study that took place in La Punta, Callao, Peru between February-April 2017. A total of six different treatments were used to disentangle chemical and biological characteristics of deep water that influence surface plankton blooms: 2 different deep water sources with different nutrient concentrations; 3 treatments to distinguish the effects of inorganic nutrients, organic nutrients and deep water microbial populations. Measured variables include inorganic…
Assessment of Sentinel-2-MSI Atmospheric Correction Processors and In Situ Spectrometry Waters Quality Algorithms
2022
The validation of algorithms developed from in situ reflectance to estimate water quality variables has the challenge of atmospheric correction (AC) when applied to satellite images. Estimating water quality variables from satellite images requires an accurate estimation of remote sensing reflectances (Rrs) which vary according to the AC applied. Validation processes for both Rrs and water quality algorithms were carried out, relating the in situ Rrs (convoluted to Sentinel-2-MSI spectral response function) with the satellite Rrs coming from different ACs (C2RCC, C2X, C2XC, and Polymer), and also relating the in situ water quality variable data with estimated water quality variable values, …
Evaluation of the spectral characteristics, purity and antioxidant activity of C-phycocyanin from the cyanobacteria collected in Kaunas Lagoon (Lithu…
2022
The physicochemical characteristics of phycocyanin extracted from cyanobacteria collected in Kaunas Lagoon were studied (spectrum characteristics, C-PC content in the dry mass and chemical purity). It was determined that the tested concentrations of C-PC in purified water should be in the range of 0.02–0.16% for measuring C-PC content in the dry mass and its spectrum characteristics. The two clear absorption maxima were detected in the spectrum of C-PC at the wavelengths of 277 and 619 nm. The content of C-PC in the dry powder form was in the range of 7.25% to 9.30% depending on its concentration in the solution and type of spectrophotometer. Furthermore, a purity factor of 1.5 was calculat…