Search results for "Pinocytosis"

1985

A block copolymer (6) with both hydrophilic and hydrophobic regions was synthesized, in order to examine its interaction with model membranes and its uptake by living cells. The copolymer comprised poly(ethylene oxide) and poly(L-lysine) with 50 mol-% substitution of the e-amino groups with palmitoyl groups. To permit 125I-labelling, p-methoxyphenyl residues (1–4 mol-%) were incorporated into the block copolymer and into a poly(ethylene oxide) used for comparison. Sudan Red 7 B solubilization studies indicated that the block copolymer, but not the homopolymer, forms micelles. Differential scanning calorimetry of dipalmitoyl-phosphatidylcholine liposomes indicated that the block copolymer in…

The closure of Pak1-dependent macropinosomes requires the phosphorylation of CtBP1/BARS

2007

Membrane fission is an essential process in membrane trafficking and other cellular functions. While many fissioning and trafficking steps are mediated by the large GTPase dynamin, some fission events are dynamin independent and involve C-terminal-binding protein-1/brefeldinA-ADP ribosylated substrate (CtBP1/BARS). To gain an insight into the molecular mechanisms of CtBP1/BARS in fission, we have studied the role of this protein in macropinocytosis, a dynamin-independent endocytic pathway that can be synchronously activated by growth factors. Here, we show that upon activation of the epidermal growth factor receptor, CtBP1/BARS is (a) translocated to the macropinocytic cup and its surroundi…

Echovirus 1 infectious entry via novel multivesicular bodies

2011

Selective uptake of naked vaccine RNA by dendritic cells is driven by macropinocytosis and abrogated upon DC maturation.

2011

Even though it is known for more than one decade that antigen-encoding RNA can deliver antigenic information to induce antigen-specific immunity against cancer, the nature and mechanism of RNA uptake have remained enigmatic. In this study, we investigated the pharmacokinetics of naked RNA administered into the lymph node. We observed that RNA is rapidly and selectively uptaken by lymph node dendritic cells (DCs). Furthermore, in vitro and in vivo studies revealed that the efficient internalization of RNA by human and murine DCs is primarily driven by macropinocytosis. Selective inhibition of macropinocytosis by compounds or as a consequence of DC maturation abrogated RNA internalization and…

Internalization of novel non-viral vector TAT-streptavidin into human cells

2007

BMC Biotechnology, 7 (1)