Search results for "Poeciliidae"
showing 2 items of 2 documents
Detection of changes in mitochondrial hydrogen sulfide i n vivo in the fish model Poecilia mexicana (Poeciliidae)
2019
ABSTRACT In this paper, we outline the use of a mitochondria-targeted ratiometric mass spectrometry probe, MitoA, to detect in vivo changes in mitochondrial hydrogen sulfide (H2S) in Poecilia mexicana (family Poeciliidae). MitoA is introduced via intraperitoneal injection into the animal and is taken up by mitochondria, where it reacts with H2S to form the product MitoN. The MitoN/MitoA ratio can be used to assess relative changes in the amounts of mitochondrial H2S produced over time. We describe the use of MitoA in the fish species P. mexicana to illustrate the steps for adopting the use of MitoA in a new organism, including extraction and purification of MitoA and MitoN from tissues foll…
Experimental Study on Diflubenzuron: Degradation in Freshwater and Bioconcentration in Mosquitofish Following Chronic Exposure
2013
ISSN: 2152-2197 ; ESSN: 2152-2219. Editeur commercial : Scientific Research Publishing; International audience; Diflubenzuron (DFB, trade name dimilin® 25 WP) is a chitin synthesis inhibitor widely used against forest insect pests in Algeria. Prior to implementation of these products as chemical agents for mosquito control, knowledge on their potential effects on non-target organisms and its behaviour in water are needed. Therefore, the present study was focused on DFB and aimed to use an HPLC procedure developed previously in order to obtain information on its degradation in freshwater and its bioconcentration in adult females of the fish Gambusia affinis (Cyprinodondiformes, Poeciliidae),…