Search results for "Polysomes"
showing 3 items of 3 documents
Inappropriate translation inhibition and P-body formation cause cold-sensitivity in tryptophan-auxotroph yeast mutants
2017
In response to different adverse conditions, most eukaryotic organisms, including Saccharomyces cerevisiae, downregulate protein synthesis through the phosphorylation of eIF2α (eukaryotic initiation factor 2α) by Gcn2, a highly conserved protein kinase. Gcn2 also controls the translation of Gcn4, a transcription factor involved in the induction of amino acid biosynthesis enzymes. Here, we have studied the functional role of Gcn2 and Gcn2-regulating proteins, in controlling translation during temperature downshifts of TRP1 and trp1 yeast cells. Our results suggest that neither cold-instigated amino acid limitation nor Gcn2 are involved in the translation suppression at low temperature. Howev…
The Saccharomyces cerevisiae Hot1p regulated gene YHR087W (HGI1) has a role in translation upon high glucose concentration stress.
2012
Abstract Background While growing in natural environments yeasts can be affected by osmotic stress provoked by high glucose concentrations. The response to this adverse condition requires the HOG pathway and involves transcriptional and posttranscriptional mechanisms initiated by the phosphorylation of this protein, its translocation to the nucleus and activation of transcription factors. One of the genes induced to respond to this injury is YHR087W. It encodes for a protein structurally similar to the N-terminal region of human SBDS whose expression is also induced under other forms of stress and whose deletion determines growth defects at high glucose concentrations. Results In this work …
Translational repression and novel functions of Cth2 in the Saccharomyces cerevisiae response to iron deficiency
2020
El hierro (Fe) es un micronutriente y cofactor esencial para todos los organismos eucariotas. Las proteínas que incorporan hierro en su estructura lo hacen en forma de grupos hemo, centros Fe/S o centros de hierro y oxígeno, entre otros. Estos cofactores están implicados en numerosos procesos celulares como la respiración, la replicación y reparación del DNA, la biogénesis de ribosomas y la traducción de proteínas, la biosíntesis de ácidos nucleicos y lípidos, la fotosíntesis y el transporte de oxígeno. Pese a ser un metal abundante en la corteza terrestre, su forma oxidada Fe+3 es la más frecuente en un entorno oxidante y resulta insoluble a pH fisiológico. Esto hace de la deficiencia de h…