Search results for "Polyvinylpolypyrrolidone"

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Design and Performance Testing of a DNA Extraction Assay for Sensitive and Reliable Quantification of Acetic Acid Bacteria Directly in Red Wine Using…

2016

International audience; Although strategies exist to prevent AAB contamination, the increased interest for wines with low sulfite addition leads to greater AAB spoilage. Hence, there is a real need for a rapid, specific, sensitive, and reliable method for detecting these spoilage bacteria. All these requirements are met by real time Polymerase Chain Reaction (or quantitative PCR; qPCR). Here, we compare existing methods of isolating DNA and their adaptation to a red wine matrix. Two different protocols for isolating DNA and three PCR mix compositions were tested to select the best method. The addition of insoluble polyvinylpolypyrrolidone (PVPP) at 1% (v/v) during DNA extraction using a pro…

0301 basic medicineMicrobiology (medical)Polyvinylpolypyrrolidone030106 microbiologyPopulationFood spoilagelcsh:QR1-502BiologyMicrobiologylcsh:MicrobiologyMatrix (chemical analysis)03 medical and health scienceschemistry.chemical_compound[SDV.IDA]Life Sciences [q-bio]/Food engineeringeducationAcetic acid bacteriaDNA extractionOriginal ResearchWineeducation.field_of_studyChromatographyRed wine[ SDV.IDA ] Life Sciences [q-bio]/Food engineeringbiology.organism_classificationDNA extraction3. Good healthMicrobiological internal controlReal time PCRReal-time polymerase chain reactionchemistryBiochemistryAcetic acid bacteriaFrontiers in Microbiology
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Polyvinylpolypyrrolidone‐Stabilized Copper Nanoparticles as an Efficient and Recyclable Heterogeneous Catalyst for the Click of 1,2,3‐Triazoles in Wa…

2019

PolyvinylpolypyrrolidonePolymers and PlasticsChemistryOrganic ChemistryNanoparticlechemistry.chemical_elementCondensed Matter PhysicsHeterogeneous catalysisCopperchemistry.chemical_compoundChemical engineeringPolymer chemistryMaterials ChemistryClick chemistryDensity functional theoryPhysical and Theoretical ChemistryMacromolecular Chemistry and Physics
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