Search results for "Porte"
showing 10 items of 1894 documents
6-mercaptopurine and 9-(2-phosphonyl-methoxyethyl) adenine (PMEA) transport altered by two missense mutations in the drug transporter gene ABCC4
2008
Multiple drug resistance protein 4 (MRP4, ABCC4) belongs to the C subfamily of the ATP-binding cassette (ABC) transporter superfamily and participates in the transport of diverse antiviral and chemotherapeutic agents such as 6-mercaptopurine (6-MP) and 9-(2-phosphonyl methoxyethyl) adenine (PMEA). We have undertaken a comprehensive functional characterization of protein variants of MRP4 found in Caucasians and other ethnicities. A total of 11 MRP4 missense genetic variants (nonsynonymous SNPs), fused to green fluorescent protein (GFP), were examined in Xenopus laevis oocytes for their effect on expression, localization, and function of the transporter. Radiolabeled 6-MP and PMEA were chosen…
Specific Binding of Baculoviruses Displaying gp64 Fusion Proteins to Mammalian Cells
2001
Viral vectors displaying specific ligand binding moieties have raised an increasing interest in the area of targeted gene therapy. In this report, we describe baculovirus vectors displaying either a functional single chain antibody fragment (scFv) specific for the carcinoembryonic antigen (CEA) or the synthetic IgG binding domains (ZZ) derived from protein A of Staphylococcus aureus. In addition, the vectors were engineered to incorporate a reporter gene encoding the enhanced green fluorescent protein (EGFP) under the transcriptional regulation of the cytomegalovirus (CMV) IE promoter. Display of the targeting moieties on the viral surface was achieved through fusion to the N-terminus of gp…
The Tobacco mosaic virus movement protein associates with but does not integrate into biological membranes
2014
Plant positive-strand RNA viruses require association with plant cell endomembranes for viral translation and replication, as well as for intra- and intercellular movement of the viral progeny. The membrane association and RNA binding of the Tobacco mosaic virus (TMV) movement protein (MP) are vital for orchestrating the macromolecular network required for virus movement. A previously proposed topological model suggests that TMV MP is an integral membrane protein with two putative -helical transmembrane (TM) segments. Here we tested this model using an experimental system that measured the efficiency with which natural polypeptide segments were inserted into the ER membrane under conditions…
Estrategias de búsqueda de artículos de revistas españolas. Estudio de un caso: evaluación de la calidad de los sistemas de información
1995
ResumenObjetivosDescribir un procedimiento sistemático para la recuperación exhaustiva de trabajos publicados en revistas españolas sobre evaluación de los sistemas de información clínicos y sanitarios (1983–1992) y analizar los términos útiles, el aporte y los fallos de las fuentes.MétodosComo fuentes para la identificación de trabajos se ha utilizado la base de datos Índice Médico Español (IME), los sumarios de revistas y la bibliografía de los trabajos recuperados. Como variables de análisis se han utilizado los términos coincidentes entre perfil de búsqueda, títulos y palabras clave; Índices de aporte, aporte específico y solapamiento; y fallos del usuario, actualización y cobertura.Res…
Health and Living Conditions of Refugees and Asylum-seekers: A Survey of Informal Settlements in Italy
2018
Newspapers and humanitarian organisations have long shown that many asylumseekers and other beneficiaries of international protection are forced to live for months or even years in precarious settlements that have sprung up spontaneously throughout Italy. However, there are no official statistics on how many asylum-seekers and refugees in Italy live this way. Our survey, carried out in 2015, is the first national survey of foreign nationals living in these makeshift camps. It aims to build a complete list of informal settlements in Italy and provide an estimate of the population size, characteristics, and living conditions of these settlements, with a focus on health conditions. The conditi…
Electrochemotherapy for advanced cutaneous angiosarcoma: A European register-based cohort study from the International Network for Sharing Practices …
2019
Abstract Background Cutaneous angiosarcoma (cAS) is a highly aggressive malignancy that challenges the radicality of surgical treatment. Electrochemotherapy (ECT), a skin-directed treatment based on cytotoxic chemotherapy combined with local electric pulses, may be an intraoperative adjunct and a new opportunity in the therapeutic strategy. This cohort study reports the experience with ECT as an option. Methods Data on patients with locally-advanced/metastatic cAS who underwent ECT between October 2013 and October 2018 at eight European centres were prospectively submitted to the InspECT (International network for sharing practices of ECT) register. Patients received therapy according to th…
HER-2/neu-mediated regulation of components of the MHC class I antigen-processing pathway.
2004
Abstract Because of its amplification and/or overexpression in many human tumors, the HER-2/neu proto-oncogene represents an attractive target for T-cell-mediated vaccination strategies. However, overexpression of oncogenes is often associated with defective expression of components of the MHC class I antigen-processing machinery (APM), thereby resulting in an immune escape phenotype of oncogene-transformed cells. To determine whether HER-2/neu influences the MHC class I antigen-processing pathway, the expression pattern of different APM components was examined in murine in vitro models of constitutive and tetracycline-controlled HER-2/neu expression. In comparison with HER-2/neu− control c…
Nitrate/oxygen co-sensing by an NreA/NreB sensor complex ofStaphylococcus carnosus
2013
In Staphylococci maximal induction of nitrate reductase (narGHJI genes) requires anaerobic conditions, the presence of nitrate, and the NreABC regulatory system. Aerobic regulation is effected by the NreB/NreC two-component system. The role of the nitrate receptor NreA in nitrate induction and its relation to aerobic regulation was analysed in Staphylococcus carnosus. Nitrate induction of a narG-lip reporter gene required presence of NreB/NreC. When nreA was deleted, nitrate was no longer required for maximal induction, suggesting that NreA is a nitrate regulated inhibitor of NreB/NreC. In vitro, NreA and mutant NreA(Y95A) decreased NreB phosphorylation in part or completely, which was due …
Rendimiento en el rendimiento entre equipos exitosos y no exitosos durante las copas del mundo de futbol de la FIFA 2010 y 2014
2015
El objetivo principal de la tesis fue el de analizar las diferencias entre equipos exitosos y no exitosos durante las dos últimas Copas del Mundo (2010 y 2014), para ello planteamos 3 objetivos específicos que nos permitieran esclarecer este problema. En esa línea específicamente tratamos de determinar las diferencias en: • La eficacia ofensiva y defensiva de los equipos (objetivo específico 1). • Los patrones de distribución temporal de los goles, el efecto del primer gol marcado (objetivo específico 2). • La posesión del balón, los pases y la eficacia de los mismos (objetivo específico 3). Muestra: La muestra fue extraída de los partidos de la fase final de las Copas del Mundo de Sudáfric…
A psychosocial reading of sport actors’ antisocial behaviours : the influence of identity processes
2021
This doctoral work is interested in stakeholder’s antisocial behaviors. This phenomenon is approached by means of the theories bound to the social categorization, and more precisely, the social identity approach and the conception of the social partitions. Following this theoretical development, two mainstudies were led, the second one drawing from the first. A third study was led to reinject the acquired knowledge in the field.The first study, articulating around five sub-studies, aimed at determining the content, the perceived causes, as well as the perceived consequences, of sport stakeholders’ antisocial behaviors. Regarding the content of antisocial behaviors, harm and disadvantage asp…