Search results for "Precipitation"

Extracellular HSP27 mediates angiogenesis through Toll-like receptor 3.

2013

The heat-shock protein 27 (HSP27) is up-regulated in tumor cells and released in their microenvironment. Here, we show that extracellular HSP27 has a proangiogenic effect evidenced on chick chorioallantoic membrane. To explore this effect, we test the recombinant human protein (rhHSP27) at physiopathological doses (0.1-10 μg/ml) onto human microvascular endothelial cells (HMECs) grown as monolayers or spheroids. When added onto HMECs, rhHSP27 dose-dependently accelerates cell migration (with a peak at 5 μg/ml) and favors spheroid sprouting within 12-24 h. rhHSP27 increases VEGF gene transcription and promotes secretion of VEGF-activating VEGF receptor type 2. Increased VEGF transcription is…

The impact of the receptor binding profiles of the vascular endothelial growth factors on their angiogenic features

2013

Abstract Background Vascular endothelial growth factors (VEGFs) are potential therapeutic agents for treatment of ischemic diseases. Their angiogenic effects are mainly mediated through VEGF receptor 2 (VEGFR2). Methods Receptor binding, signaling, and biological efficacy of several VEGFR2 ligands were compared to determine their characteristics regarding angiogenic activity and vascular permeability. Results Tested VEGFR2 ligands induced receptor tyrosine phosphorylation with different efficacy depending on their binding affinities. However, the tyrosine phosphorylation pattern and the activation of the major downstream signaling pathways were comparable. The maximal angiogenic effect stim…

2019

Abstract. The cumulative occurrence of heavy precipitation and flood events during recent years in various Central European locations emphasises the urgent need to improve extreme rainfall observations and forecasts. Precipitation gauges based on a weighing system allow the recording of intense short-term precipitation events with a very high temporal resolution (down to 1 min). In this study, observational data that were collected during the period 2000 to 2016 for 126 stations of two corresponding measuring networks in the Central German state of Hesse were investigated for the first time to answer the following questions: (1) Are the recorded high-resolution precipitation data plausible …

Epigenetic Status of an Adenovirus Type 12 Transgenome upon Long-Term Cultivation in Hamster Cells

2007

ABSTRACT The epigenetic status of integrated adenovirus type 12 (Ad12) DNA in hamster cells cultivated for about 4 decades has been investigated. Cell line TR12, a fibroblastic revertant of the Ad12-transformed epitheloid hamster cell line T637 with 15 copies of integrated Ad12 DNA, carries one Ad12 DNA copy plus a 3.9-kbp fragment from a second copy. The cellular insertion site for the Ad12 integrate, identical in both cell lines, is a >5.2-kbp inverted DNA repeat. The Ad12 transgenome is packaged around nucleosomes. The cellular junction is more sensitive to micrococcal nuclease at Ad12-occupied sites than at unoccupied sites. Bisulfite sequencing reveals complete de novo methylation i…

Fat storing cells (FSC) of rat liver synthesize and secrete fibronectin

1987

Summary Fat storing cells (FSC) of rat liver were isolated and kept in culture for up to 2 weeks. Freshly isolated cells and cells in culture were characterized functionally (vitamin A content) and morphologically. Their synthetic capacity for fibronectin was studied quantitatively by ELISA-measurement and qualitatively by biosynthetic labeling, immunoprecipitation and SDS-PAGE analysis. The synthesis product and the kinetics of the synthesis and secretion were compared with those obtained from culture of hepatocytes. FSC were shown to synthesize and secrete fibronectin under the culture conditions used. No fibronectin synthesis was detected during the first 3 days of culture. Thereafter th…

Hypoxia Positively Regulates the Expression of pH-Sensing G-Protein–Coupled Receptor OGR1 (GPR68)

2016

Background & Aims: A novel family of proton-sensing G-proteinâcoupled receptors, including ovarian cancer G-proteinâcoupled receptor 1 (OGR1) (GPR68) has been identified to play a role in pH homeostasis. Hypoxia is known to change tissue pH as a result of anaerobic glucose metabolism through the stabilization of hypoxia-inducible factor-1α. We investigated how hypoxia regulates the expression of OGR1 in the intestinal mucosa and associated cells. Methods: OGR1 expression in murine tumors, human colonic tissue, and myeloid cells was determined by quantitative reverse-transcription polymerase chain reaction. The influence of hypoxia on OGR1 expression was studied in monocytes/macrophages and…

Water stable isotope data set in temperate, lowland catchment, two years of monthly observations, River Salaca, Latvia

2020

Highlights • Two years of monthly water stable isotope ratios from temperate lowland catchment. • Hourly water table, temperature and electrical conductivity monitoring. • Distinct isotope ratios for raised bog, groundwater and rivers.

Weather circulation patterns as precursor of heavy rainfall events: an application to Sicily, Italy

2020

Since the impacts of climate change on the environment have been constantly rising over the last decades, scientists have paid much attention to understanding the effects of this phenomenon. Climate change leads to different kinds of extremes, such as heavy rainfall events, characterized by short duration and high intensity, and drought, which can cause the problem of water scarcity over a certain area. These types of extreme events cause several damages for the affected areas since they can result in loss of human lives and economic damages. In particular, heavy rainfall events, which are often associated with convective precipitation because of their characteristics, may result in flash f…

Episodios de precipitación torrencial en el este y sureste ibéricos y su relación con la variabilidad intraanual de la oscilación del Mediterráneo oc…

2018

Ponencia presentada en: XI Congreso de la Asociación Española de Climatología celebrado en Cartagena entre el 17 y el 19 de octubre de 2018. [ES]La relación de la Oscilación del Mediterráneo Occidental (WeMO) con la ocurrencia e intensidad de las precipitaciones en el litoral mediterráneo ibérico ha sido demostrada en numerosos trabajos. Entre 1950 y 2016 se registraron 239 episodios de precipitación torrencial extrema (≥200 mm/24 horas) en las cuencas hidrográficas del Júcar y del Segura (3,6 casos al año). El 29,3% de estos eventos tuvieron lugar con una fase de la WeMO fuertemente negativa, el 37,5% en una fase negativa y el 28,3% en una fase ligeramente negativa. Solo el 7,9% de los epi…

Seasonal and Inter-annual Variation of Evapotranspiration in Amazonia Based on Precipitation, River Discharge and Gravity Anomaly Data

2019

We analyzed seasonal and spatial variations of evapotranspiration (ET) for five Amazon sub-basins and their response to the 2015/16 El Nino episode using a recently developed water-budget approach. ET varied typically between similar to 7 and 10 cm/month with exception of the Xingu basin for which it varied between 10 and 15 cm/month. Outstanding features of ET seasonality are (i) generally weak seasonality, (ii) two ET peaks for the two very wet catchments Solimoes and Negro, with one occurring during the wet season and one during the drier season, and (iii) a steady increase of ET during the second half of the dry season for the three drier catchments (Madeira, Tapajos, Xingu). Peak ET oc…