Search results for "Primer"

Podria Descartes embarcar en la nau de Neurath?

1999

En aquest article Josep Lluís Blasco reflexiona sobre els suggeriments epistemològics de Descartes, des de la perspectiva de la teoria del coneixement del segle XX, i es pregunta fins a quin punt es pot considerar que Descartes inaugurà l’epistemologia moderna i quina és la vigència del seu legat. Enceta la seua anàlisi amb la metàfora de la nau d’Otto Neurath, segons la qual el coneixement científic no té uns fonaments sòlids perquè és un constructe teòric que s’ha de revisar de manera contínua, i afegeix la modificació que en fa Quine en situar a la mateixa nau la filosofia i la ciència. Blasco examina si Descartes podria embarcar en dita nau, i conclou que la resposta no és tan senzilla …

Molecular analysis ofPhytophthoradiversity in nursery-grown ornamental and fruit plants

2015

The genetic diversity of Phytophthora spp. was investigated in potted ornamental and fruit tree species. A metabarcoding approach was used, based on a semi-nested PCR with Phytophthora genus-specific primers targeting the ITS1 region of the rDNA. More than 50 ITS1 sequence types representing at least 15 distinct Phytophthora taxa were detected. Nine had ITS sequences that grouped them in defined taxonomic groups (P. nicotianae, P. citrophthora, P. meadii, P. taxon Pgchlamydo, P. cinnamomi, P. parvispora, P. cambivora, P. niederhauserii and P. lateralis) whereas three phylotypes were associated to two or more taxa (P. citricola taxon E or III; P. pseudosyringae, P. ilicis or P. nemorosa; and…

Rubberlike elasticity?a molecular primer. ByJ. E. Mark andB. Erman. Wiley, Chichester 1988. viii, 196 pp., bound, � 23.70.?ISBN 0-471-61499-8

1989

MOLECULAR ANALYSIS OF PHYTOPHTHORA DIVERSITY IN ORNAMENTAL NURSERIES.

2013

A molecular approach based on the use of genus-specific nested-PCR primers (Scibetta et al., 2012; J. Microbiol. Meth. 88: 356-368) was utilized to detect Phytophthora species in soil and root samples of potted ornamentals, collected across Apulia and Calabria, Southern Italy. Analyzed samples comprised many plant species with different levels of symptoms of decline on the canopy and root rots. Extraction protocols were optimized to obtain DNA samples of appropriate quality from soil and roots. The analysis of sequences after cloning of nested-PCR amplicons enabled the identification of different Phytophthora species including P. nicotianae, P. cinnamomi, P. cryptogea, P. palmivora and P. n…

Identification of Phytophthora species infecting citrus in Vietnam

2013

In a survey aimed at identifying Phytophthora species infecting fruit crops in Viet Nam, P. citrophthora and P. nicotianae were the species most commonly recovered from soil and trees in citrus plantations. P. nicotianae was prevalent in citrus groves in the Ben Tre and Tien Giang provinces (southern Viet Nam). Interestingly, both A1 and A2 mating types of the latter species were found, while in other citrus-growing areas of the world, such as the Mediterranean region, South Africa, Australia and the Americas, A1 is the only mating type occurring in citrus groves, suggesting southern-east Asia could be a centre of origin of this Phytophthora species. Mixed infections of three Phytophthora s…

Noncanonical RNAs from transcripts of the Drosophila muscleblind gene.

2006

It has become increasingly evident that eukaryotic cells produce RNA molecules from coding genes with constitutions other than those of typically spliced mRNA transcripts. Here we describe new cDNAs from the Drosophila melanogaster muscleblind (mbl ) locus that identify two such atypical RNA molecules: RNAs containing an incomplete exon 2 tandem repetition (mblE2E2#) or having exons with a different order compared to the corresponding genomic DNA (mblE2E3#E2#; exon scrambling). The existence of exon duplications and rearrangements in the genomic locus that might explain such cDNAs was ruled out by genomic Southern blotting and in silico analysis of the Drosophila genome sequence. The incomp…

Influence of the xyloadenosine analogue of 2?,5?-oligoriboadenylate on poly(A)-specific, 2?,5?-oligoriboadenylate degrading 2?,3?-exoribonuclease and…

1984

The homogeneous poly(A)-specific 2′,3′-exoribonuclease from calf thymus gland, which cleaves both 3′,5′-and 2′,5′-linked oligoriboadenylates, does not degrade (xyloA2'p)2 xyloA, the xylofuranosyladenosine analogue of the 2-5A core. This oligonucleotide, which is supposed to enter intact cells rapidly, was found to possess an increased stability and an enhanced antiherpesvirus activity compared to the natural (A2'p)2A (Eppstein, D. A., Barnett, J. W., Marsh, Y. V., Gosselin, G. and Imbach, J.-L. (1983) Nature 302, 723–724). The poly(A) anabolic enzyme, poly(A) polymerase (Mn2+-dependent), from the same source, which is initiated by (A3'p)2A and its higher oligomers, does not accept 2–5A core…

DNA Polymerase Action on Oligonucleotide Templates from Human Ha-rasProtooncogene Containing N6-Deoxyadenosine Adducts Derived from Trans Addition of…

1996

Abstract In the present work we have used a DNA polymerase assay to investigate the primer extension with T7 DNA polymerase (Sequenase 2.0) and the Klenow fragment of Escherichia coli DNA polymerase I (exo − KF) on chemically synthesized 21mer templates representing partial sequences of the human Ha-ras protooncogene with site-specifically positioned trans-N 6-dA adducts of (-)- (adduct 1) and (+)-anti-benzo[c]phenanthrene 3,4-dihydrodiol 1,2-epoxides (adduct 2) at codon 61 (CA∗G; A∗ indicates the adducted position). With Sequenase 2.0 a complete block of primer extension opposite both adduct 1 and 2 was noted using a 10mer primer reaching the (n-1)-position of the adduct. A detailed analys…

A multiplex polymerase chain reaction protocol for the simultaneous analysis of the glutathione S-transferase GSTM1 and GSTT1 polymorphisms.

1996

Inhibition Probing on a Cut-Edge Electrode Machined from a Primer-Coated 2024-T3 Alloy

2013

Conventional corrosion protection of 2024-T3 alloys (AA2024T3) in aeronautic structures combines conversion coating with chromate-inhibited primer protected by a top-coat. In some cases this protection can break down after mechanical damage to the paint systems inducing a risk of localized corrosion if the release of inhibitors trapped in the primer does not reach a critical level. There is a need for developing computational models combining the inhibitor release and transport within and from the coating system andthecorrosioninhibitionprocess,toassistinthedesignofnewmultifunctionalcoatingsystems.Theinfluenceonthecorrosioninhibition of various parameters such inhibitor release rate, defect…