Search results for "Profilin"

Characterization and transcription studies of a phytochelatin synthase gene from the solitary tunicate Ciona intestinalis exposed to cadmium.

2014

The major thiol-containing molecules involved in controlling the level of intracellular ROS in eukaryotes, acting as a nonenzymatic detoxification system, are metallothioneins (MTs), glutathione (GSH) and phytochelatins (PCs). Both MTs and GSH are well-known in the animal kingdom. PC was considered a prerogative of the plant kingdom but, in 2001, a phytochelatin synthase (PCS) gene was described in the nematode Caenorhabditis elegans; additional genes encoding this enzyme were later described in the earthworm Eisenia fetida and in the parasitic nematode Schistosoma mansoni but scanty data are available, up to now, for Deuterostomes. Here, we describe the molecular characteristics and transc…

Application of an immunoproteomic approach to detect anti-profilin antibodies in sera of paritaria judaica allergic patients

2011

Pollen from grasses, weeds, and trees constitutes one of the main sources of inhalant allergens frequently associated with seasonal patterns of allergic diseases. Pollen allergens show some analogies in the amino acids sequence which determine immunological similarity and cross reactivity. Parietaria judaica (P.j) pollen represents one of the main sources of allergens in the Mediterranean area and its major allergens have already been identified (Par j 1 and Par j 2). Recently, has been also described a minor allergen, profilin (Par j 3), an allergen present in pollen of trees, grasses and weeds. Allergenic plant profilins constitute a highly conserved family with sequence identities of 70%…

Regulatory networks underlying mycorrhizal development delineated by genome-wide expression profiling and functional analysis of the transcription fa…

2017

Background: Ectomycorrhizal (ECM) fungi develop a mutualistic symbiotic interaction with the roots of their host plants. During this process, they undergo a series of developmental transitions from the running hyphae in the rhizosphere to the coenocytic hyphae forming finger-like structures within the root apoplastic space. These transitions, which involve profound, symbiosis-associated metabolic changes, also entail a substantial transcriptome reprogramming with coordinated waves of differentially expressed genes. To date, little is known about the key transcriptional regulators driving these changes, and the aim of the present study was to delineate and functionally characterize the trans…

Institutional perspectives in transition : research groups’ profiles and embeddedness in organisational and national context

2019

Research into differentiation and profiling of knowledge producing institutions through the lenses of institutional logics and field embeddedness have proliferated in recent years. By discussing this process in the context of research groups, as those basic units in which knowledge production epistemically and practically takes place, this article offers a contribution to the theoretical discussion on organisational differentiation. Based on a small-N comparative case study of research groups operating in different national and organisational contexts on a single, highly competitive and interdisciplinary applied sciences field, nanosciences, the article proposes research group profiles as h…

Continuous Requirements Risk Profiling in Information Systems Development

2015

With the increasing adoption of agile, lean, and iterative development methods, information systems development (ISD) has become continuous, meaning that system development moves rapidly from release to release. This means that work practices and challenges that practitioners face have changed. Despite these changes, requirements development is still critical in ISD. However, IS literature is silent on how to manage requirements-related risks in the practice of continuous IS development. To fill this gap, we propose a continuous requirements risk profiling method. The study is informed by design science research methodology, and we apply focus group interviews and a Delphi study for data co…

Choosing a study mode in blended learning

2012

Education providers aim to meet today's education requirements by employing, among other things, education technology solutions that increase flexibility. This has also happened with the master's level mathematical information technology degree directed to adults. In the degree program, lecture videos brought in together with face-to-face teaching provide good opportunities for flexible educational arrangements. Education with the help of videos can be arranged in such a way that students themselves will be able to choose their study mode in accordance with their needs. When students themselves can choose their study modes, many different ways to take advantage of the flexibility provided a…

Observed Classroom Quality Profiles of Kindergarten Classrooms in Finland

2012

Research Findings: The aim of the present study was to examine classroom quality profiles of kindergarten classrooms using a person-centered approach and to analyze these patterns in regard to teacher and classroom characteristics. Observations of the domains of Emotional Support, Classroom Organization, and Instructional Support were conducted in 49 Finnish kindergarten classrooms utilizing the Classroom Assessment Scoring System (R. C. Pianta, K. M. LaParo, & B. K. Hamre, 2008 ). In addition, questionnaire data on classroom and teacher characteristics, as well as Early Childhood Classroom Observation Measure (D. Stipek & P. Byler, 2004 ) observational ratings, were used in the analyses. L…

Identification of mycorrhiza-regulated genes with arbuscule development-related expression profile

2004

Suppressive subtractive hybridisation was applied to the analysis of late stage arbuscular mycorrhizal development in pea. 96 cDNA clones were amplified and 81, which carried fragments more than 200 nt in size, were sequence analysed. Among 67 unique fragments, 10 showed no homology and 10 were similar to sequences with unknown function. RNA accumulation of the corresponding 67 genes was analysed by hybridisation of macro-arrays. The cDNAs used as probes were derived from roots of wild type and late mutant pea genotypes, inoculated or not with the AM fungus Glomus mosseae. After calibration, a more than 2.5-fold mycorrhiza-induced RNA accumulation was detected in two independent experiments…

Regulation of type 1 fimbriae synthesis and biofilm formation by the transcriptional regulator LrhA of Escherichia coli

2005

Type 1 fimbriae ofEscherichia colifacilitate attachment to the host mucosa and promote biofilm formation on abiotic surfaces. The transcriptional regulator LrhA, which is known as a repressor of flagellar, motility and chemotaxis genes, regulates biofilm formation and expression of type 1 fimbriae. Whole-genome expression profiling revealed that inactivation oflrhAresults in an increased expression of structural components of type 1 fimbriae.In vitro, LrhA bound to the promoter regions of the twofimrecombinases (FimB and FimE) that catalyse the inversion of thefimApromoter, and to the invertible element itself. TranslationallacZfusions with these genes and quantification offimEtranscript le…

Pricavy-Preserving Aspects for Data Mining in ICT Services

The steady adoption of systems for profiling users behavior, collecting and critically interpreting as much information as possible about likes and dislikes, interests and habits of Internet residents and generic services consumers have rapidly become some of the hottest keywords within networking research community. Indeed, mining information about users behavior is an advantage for both service providers and service customers: on one side, providers can improve their revenues by focusing on the most successful features of their services, while on the other side, users can enjoy services which reflect closer their specific needs. There are many examples of user profiling applications. Inte…