Search results for "Proflavine"

showing 3 items of 3 documents

Computational study of the interaction of proflavine with d(ATATATATAT)2 and d(GCGCGCGCGC)2

2009

Abstract The interaction of proflavine (PR) with two B-DNA decamers of alternating AT and GC sequence, called [deca(dG-dC)]2 and [deca(dA-dT)]2, respectively, was computationally investigated by the ONIOM method, exploiting a three-layer QM/QM/MM hybrid approach. The highest level QM method was applied to the model system, which comprises the intercalation site (5th and 6th base pairs) and the inserted PR molecule. The connecting sugar phosphate backbone was added in the medium layer region. Both higher and medium level layers, differing in the size of the basis set used, were treated by the DFT MPWB1K functional. The full system in the lower layer was described by the empirical AMBER force…

ONIOMChemistryIntercalation (chemistry)Poly(dA-dT)Interaction energyCondensed Matter PhysicsBiochemistryDFTQM/MMQM/MMCrystallographychemistry.chemical_compoundComputational chemistrySettore CHIM/03 - Chimica Generale E InorganicaMoleculePhysical and Theoretical ChemistryPoly(dG-dC)Deca-ProflavineBasis setProflavine
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Ag2 and Ag3 clusters: synthesis, characterization, and interaction with DNA.

2015

Subnanometric samples, containing exclusively Ag2 and Ag3 clusters, were synthesized for the first time by kinetic control using an electrochemical technique without the use of surfactants or capping agents. By combination of thermodynamic and kinetic measurements and theoretical calculations, we show herein that Ag3 clusters interact with DNA through intercalation, inducing significant structural distortion to the DNA. The lifetime of Ag3 clusters in the intercalated position is two to three orders of magnitude longer than for classical organic intercalators, such as ethidium bromide or proflavine. Fil: Buceta, David. Universidad de Santiago de Compostela; España Fil: Busto, Natalia. Unive…

SilverStereochemistryFísico-Química Ciencia de los Polímeros ElectroquímicaIntercalation (chemistry)electrochemical synthesiElectrochemistryCatalysisCatalysiscluster compoundchemistry.chemical_compoundintercalationElectrochemistryProflavineDNA synthesisChemistryChemistry (all)Ciencias QuímicasGeneral ChemistryGeneral MedicineDNAXANESXANESCharacterization (materials science)CrystallographyOrders of magnitude (time)BiochemistrySettore CHIM/03 - Chimica Generale E InorganicaEthidium bromideCLUSTERSKINETIC CONTROLCIENCIAS NATURALES Y EXACTASDNAAngewandte Chemie (International ed. in English)
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Flow-injection analysis study of the chemiluminescent behaviour of proflavine and acriflavine

2001

Abstract The chemiluminescent behaviour of the amino acridines like acriflavine, and proflavin is reported. Different strong oxidants (potassium permanganate, cerium(IV), hexacyanoferrate(III), hydrogen peroxide in different media) were tested, and potassium permanganate in sulphuric acid medium was selected. The study of the experimental parameters affecting the oxidation and detection was performed with the aid of a FIA assembly. The calibration graph was applied over the range 0.05–12.0 μg ml −1 of acriflavine (regression coefficient 0.9957, and the calculated relative standard deviation (R.S.D., %) was 0.9; LOD 10 ng ml −1 and the sample throughput 48 h −1 . The study of interfering com…

Flow injection analysisCalibration curveInorganic chemistryAnalytical chemistryBiochemistryAnalytical Chemistrylaw.inventionAcridonechemistry.chemical_compoundPotassium permanganatechemistrylawEnvironmental ChemistryAcriflavineHydrogen peroxideSpectroscopyProflavineChemiluminescenceAnalytica Chimica Acta
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