Search results for "Prom"

Structural Insight into CVB3-VLP Non-Adjuvanted Vaccine

2020

Coxsackievirus B (CVB) enteroviruses are common pathogens that can cause acute and chronic myocarditis, dilated cardiomyopathy, aseptic meningitis, and they are hypothesized to be a causal factor in type 1 diabetes. The licensed enterovirus vaccines and those currently in clinical development are traditional inactivated or live attenuated vaccines. Even though these vaccines work well in the prevention of enterovirus diseases, new vaccine technologies, like virus-like particles (VLPs), can offer important advantages in the manufacturing and epitope engineering. We have previously produced VLPs for CVB3 and CVB1 in insect cells. Here, we describe the production of CVB3-VLPs with enhanced pro…

Casein kinase 2 inhibits HomolD-directed transcription by Rrn7 in Schizosaccharomyces pombe.

2014

In Schizosaccharomyces pombe, ribosomal protein gene (RPG) promoters contain a TATA analogue element called the HomolD box. The HomolD-binding protein Rrn7 forms a complex with the RNA polymerase II machinery. Despite the importance of ribosome biogenesis to cell survival, the mechanisms involved in the regulation of transcription of eukaryotic RPGs are unknown. In this study, we identified Rrn7 as a new substrate of the pleiotropic casein kinase 2 (CK2), which is a regulator of basal transcription. Recombinant Rrn7 from S. pombe, which is often used as a model organism for studying eukaryotic transcription, interacted with CK2 in vitro and in vivo. Furthermore, CK2-mediated phosphorylation…

Functional characterization of the enhancer blocking element of the sea urchin early histone gene cluster reveals insulator properties and three esse…

2000

Insulator elements can be functionally identified by their ability to shield promoters from regulators in a position-dependent manner or their ability to protect adjacent transgenes from position effects. We have previously reported the identification of a 265 bp sns DNA fragment at the 3' end of the sea urchin H2A early histone gene that blocked expression of a reporter gene in transgenic embryos when placed between the enhancer and the promoter. Here we show that sns interferes with enhancer-promoter interaction in a directional manner. When sns is placed between the H2A modulator and the inducible tet operator, the modulator is barred from interaction with the basal promoter. However, th…

The adolescents’ school engagement: Assessment of its dimensions

2016

A topic that in the last years is getting relevance in school and students research is school engagement. This topic has been related, among other important constructs, with burnout of teachers and students, school performance, satisfaction with the school, behavioral disruption, goal orientation and motivational climate in the classroom, students-teachers relationships, and life satisfaction of the students. School engagement may be defined as the participation of the student in academic achievements, and it is understood as a multidimensional construct. The most repeated typology of dimensions recognizes three specific and overlapping dimensions: cognitive, behavioral, and emotional (affe…

Biologically active organic compounds, especially plant promoters, in algae extracts and their potential application in plant cultivation

2015

Scientific efforts taken during the past decades undoubtedly have proven that algae are organisms wealthy in various chemical substances such as carbohydrates, proteins, and minerals, as well as polysaccharides, polyphenols, phlorotannins, pigments, unsaturated fats, sterols, and of course growth promoting substances like phytohormones. It is noteworthy that among the many biologically active compounds obtained from seaweeds, plant hormones seem to be the most essential for enhancing the growth and development of crop plants. This fact is well reflected in over 60 years of the long history of controlled application of algae origin products as natural fertilizers in crop production. Neverthe…

Adapting Net Promoter thinking in public sector organizations

2016

Tässä maisterintutkielmassa tarkasteltiin Net Promoter score (NPS) -suosittelumittarin sopivuutta julkissektorin käyttöön. Tutkielman tavoitteena oli selvittää, sopiiko yksityisellä sektorilla paljon käytetty mittari julkissektorin organisaatioihin. Tutkimus täyttää aukon tutkimuskentällä, jossa NPS:ää on tutkittu paljon yksityisen sektorin organisaatioissa, mutta tutkimusta julkissektorilta ei tiettävästi ole tehty. Tutkimuskysymykset olivat: 1) Millaisia piirteitä julkissektorin suosittelijoilla on; 2) millaisia piirteitä julkissektorin herjaajilla on; 3) millaisia piirteitä julkissektorin passiiveilla on. Teoreettinen viitekehys käsittelee julkissektorin maine- ja tyytyväisyysmittareita …

“Can do!” Teacher Promotion of Optimism in Response to Student Failure Expectation Expressions in Classroom Discourse

2019

This study offers detailed observational analyses of how teachers use optimism as an instructional resource when responding to students’ failure expectation displays in classroom situations. The results were based on video-recordings of 25 lessons in two Finnish part-time special education settings, analysed by means of applied conversation analysis. The results showed that the teachers boosted optimism by inverting students’ negative utterances, giving examples of successful experiences of peers, offering praise for students’ earlier performance or focusing on problems through instructional support. The study underlines the importance of addressing and elaborating students’ negative learni…

Hands-on math and art exhibition promoting science attitudes and promoting science attitudes

2017

Are Facebook brand community members truly loyal to the brand?

2015

Investigates two Facebook brand communities and their relationship with loyalty.Community commitment is associated with community promotion behavior and loyalty.Community promotion behavior is associated with different types of loyalty.User activity in Facebook has little or no effect on brand loyalty. This study examines members of Facebook brand communities and tests the relationships between their commitment to the community and brand loyalty. A conceptual model on the linkages between brand community commitment, community promotion behavior and brand loyalty including attitudinal loyalty, repurchase intention and positive word-of-mouth (WOM) is developed and tested with two samples. Dat…

Rola serwisu internetowego w promocji działalności towarzystw bibliofilskich

2007

Towarzystwa bibliofilskie, aby rozwijać się w XXI w., muszą dotrzeć do społeczeństwa za pomocą Internetu. Obecnie dużą rolę w propagowaniu działalności na polu miłośnictwa książek odgrywa posiadanie własnego serwisu WWW. W artykule uzasadniono potrzebę promocji towarzystw bibliofilskich w Internecie. Omówiono skuteczność wykorzystania w tym celu serwisu internetowego. Przedstawiono możliwości jego zastosowania w działalności promocyjnej oraz public relations na przykładzie serwisu Rycerskiego Zakonu Bibliofilskiego z Kapitułą Orderu Białego Kruka w Krakowie, który powstał w ramach pracy magisterskiej autorki artykułu. Omówiono projekt serwisu oraz etapy jego tworzenia. Przedstawiono wyniki …