Search results for "Prom"
showing 10 items of 2286 documents
Aggiunte al catalogo di Bonaventura Caruso, sacerdote e orafo messinese della seconda metà del ‘700
2011
Il nome di Bonaventura Caruso, sacerdote e orafo messinese, compare per la prima volta intorno agli anni '70 del XVIII secolo. L'autore, attraverso lo studio delle fonti e grazie a un'accurata analisi stilistica, propone l'identificazione e l'attribuzione a Caruso di un gruppo di opere di argenteria sacra prodotta a Messina oggi nei tesori delle Chiese Madri di Geraci Siculo e Mistretta, tutte caratterizzate dalla presenza di forme e motivi decorativi rococò, ma già tendenti al neoclassicismo. The name of Bonaventura Caruso, a priest and goldsmith from Messina, first appears around the 1870s. The author, through the study of sources and thanks to careful stylistic analysis, proposes the ide…
[Diari del Compromís de Casp]
Ms Títol donat pel catalogador Col·lació: Paper, f. i (pergamí actual) + 156 (en blanc els f. 151r-156v) + i (paper) ; foliació moderna a llapis ; signatures de quadern II-VI Composició: Escrit a línia tirada a 27 i 28 línies Escriptura: Gòtica cursiva. Notes marginals d'altra mà (del notari Jaume Desplà) Enquadernació: Pergamí del segle XV, restaurada en 2011 Origen: Escrit a Casp, Saragossa, l'any 1412 després del 28 de juny, data de publicació de la Sentència en la qual es nomena a l'infant de Castella, Ferran, com a rei de la Corona d'Aragó Signatura anterior: Olim 40 Signatura actual: València, Universitat de València. Biblioteca Històrica, BH Ms. 40 Catalogat des del manuscrit origina…
Termination of transcription in an ‘in vitro’ system is dependent on a polyadenylation sequence
1991
Using HeLa cell nuclear extract as a source of the different transcription and polyadenylation factors and reverse transcription to analyze the levels of RNA 5' and 3' to the cleavage-polyadenylation site, an in vitro assay has been established to study polyadenylation coupled to transcription directed by different adenovirus promoters. The levels of transcription 5' and 3' to the cleavage site in the L3 polyadenylation region are practically the same as described previously, however, the level of transcription 3' to the cleavage site in the SV40 early polyadenylation region decreases immediately after the cleavage site indicating a termination of the transcription.
Nuclear receptors modulate the interaction of Sp1 and GC-rich DNA via ternary complex formation
2000
Binding sites for transcription factor Sp1have been implicated in the transcriptional regulation of several genes by hormones or vitamins, and here we show that a GC-rich element contributes to the retinoic acid response of the interleukin 1β promoter. To explain such observations, it has been proposed that nuclear receptors can interact with Sp1 bound to GC-rich DNA. However, evidence supporting this model has remained indirect. So far, nuclear receptors have not been detected in a complex with Sp1 and GC-rich DNA, and the expected ternary complexes in non-denaturing gels were not seen. In search for these missing links we found that nuclear receptors [retinoic acid receptor (RAR), thyroid…
A novel SP-1 site in the human interleukin-1β promoter confers preferential transcriptional activity in keratinocytes
1996
To investigate the mechanisms of transcriptional activation of interleukin-1beta (IL-1beta) in non-monocytic cells, we constructed a series of reporter plasmids with the bacterial chloramphenicol acetyltransferase gene linked to various parts of the human IL-1beta promoter and performed transient transfection experiments. We identified a promoter segment that activates transcription most efficiently in keratinocytes. Electrophoretic mobility shift assays (EMSA) with a 43-mer oligonucleotide derived from the functionally identified cis-acting element revealed specific complexes. By competition analysis with transcription factor consensus sequence oligonucleotides and by immunosupershift, tra…
EngineeredControl of Cell Morphology In Vivo Reveals Distinct Roles for Yeast andFilamentous Forms of Candida albicans duringInfection
2003
ABSTRACT It is widely assumed that the ability of Candida albicans to switch between different morphologies is required for pathogenesis. However, most virulence studies have used mutants that are permanently locked into either the yeast or filamentous forms which are avirulent but unsuitable for discerning the role of morphogenetic conversions at the various stages of the infectious process. We have constructed a strain in which this developmental transition can be externally modulated both in vitro and in vivo. This was achieved by placing one copy of the NRG1 gene (a negative regulator of filamentation) under the control of a tetracycline-regulatable promoter. This modified strain was th…
Computational identification of cell-specific variable regions in ChIP-seq data.
2019
ABSTRACT Chromatin immunoprecipitation followed by sequencing (ChIP-seq) is used to identify genome-wide DNA regions bound by proteins. Several sources of variation can affect the reproducibility of a particular ChIP-seq assay, which can lead to a misinterpretation of where the protein under investigation binds to the genome in a particular cell type. Given one ChIP-seq experiment with replicates, binding sites not observed in all the replicates will usually be interpreted as noise and discarded. However, the recent discovery of high-occupancy target (HOT) regions suggests that there are regions where binding of multiple transcription factors can be identified. To investigate these regions,…
Electron microscopic demonstration of intracelluar promethazine accumulation sites by a precipitation technique: application to the cerebellar cortex…
1996
A method is described that allows electron microscopic identification of the phenothiazine neuroleptic promethazine after supravital intracardiac injection of high drug concentrations (greater than or equal to 3 %). The cerebellar cortex of the mouse was used for the investigation. This procedure is based on simultaneous fixation of drug and tissue by immersion in a paraformaldehyde-glutaraldehyde solution with the addition of phosphomolybdic acid. The electron microscopic investigation revealed that the drug could easily be identified as an electron-dense precipitate. Subpopulations of neurons exhibited a higher affinity for the drug than others, but no preference for any nerve cell type …
Differential expression levels of Sox9 in early neocortical radial glial cells regulate the decision between stem cell maintenance and differentiation
2021
ABSTRACTRadial glial progenitor cells (RGCs) in the dorsal forebrain directly or indirectly produce excitatory projection neurons and macroglia of the neocortex. Recent evidence shows that the pool of RGCs is more heterogeneous than originally thought and that progenitor subpopulations can generate particular neuronal cell types. Using single cell RNA sequencing, we have studied gene expression patterns of two subtypes of RGCs that differ in their neurogenic behavior. One progenitor type rapidly produces postmitotic neurons, whereas the second progenitor remains relatively quiescence before generating neurons. We have identified candidate genes that are differentially expressed between thes…