Search results for "Protein Array Analysis"

showing 2 items of 32 documents

Serological Levels of Anti-clathrin Antibodies Are Decreased in Patients With Pseudoexfoliation Glaucoma

2021

Evidence for immunologic contribution to glaucoma pathophysiology is steadily increasing in ophthalmic research. Particularly, an altered abundance of circulating autoantibodies to ocular antigens is frequently observed. Here, we report an analysis of autoantibody abundancies to selected antigens in sera of open-angle glaucoma patients, subdivided into normal-tension glaucoma (N = 31), primary open-angle glaucoma (N = 43) and pseudoexfoliation glaucoma (N = 45), vs. a non-glaucomatous control group (N = 46). Serum samples were analyzed by protein microarray, including 38 antigens. Differences in antibody levels were assessed by ANOVA. Five serological antibodies showed significantly altered…

lcsh:Immunologic diseases. Allergy0301 basic medicineMicroarraygenetic structuresautoantibodiesImmunologyProtein Array AnalysisGlaucomamedicine.disease_causeExfoliation SyndromeAutoantigensSerologyAutoimmunityPathogenesis03 medical and health sciences0302 clinical medicineAntigenmedicineHumansImmunology and AllergyOriginal Researchbiologybusiness.industryautoimmunityAutoantibodyComputational Biologybioinformaticsmedicine.diseaseimmunoproteomicseye diseasesClathrin030104 developmental biologyglaucomaCase-Control StudiesImmunology030221 ophthalmology & optometrybiology.proteinsense organsDisease SusceptibilityAntibodylcsh:RC581-607businessmicroarrayBiomarkersGlaucoma Open-AngleFrontiers in Immunology
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Embryologic outcome and secretome profile of implanted blastocysts obtained after coculture in human endometrial epithelial cells versus the sequenti…

2008

Objective To compare embryologic and clinical outcomes in terms of preimplantation development, implantation, pregnancy rates, and secretome profile of implanted blastocysts from the preimplantation genetic diagnosis program grown in sequential versus endometrial epithelial cell (EEC) coculture system. Design Retrospective clinical study and prospective experimental study. Setting In vitro fertilization clinical unit and university research laboratory. Intervention(s) Blastomere biopsy, embryo culture, blastocyst transfer, and protein analysis of the media conditioned from implanted embryos obtained from coculture and sequential systems. Main Outcome Measure(s) Clinical study: blastocyst, i…

medicine.medical_specialtymedicine.medical_treatmentProtein Array AnalysisFertilization in VitroBiologyPreimplantation genetic diagnosisEndometriumAndrologyEmbryo Culture TechniquesEndometriumPregnancymedicineSingle Embryo TransferHumansBlastocystEmbryo ImplantationPreimplantation DiagnosisRetrospective StudiesGynecologyIn vitro fertilisationOocyte DonationInterleukin-6Blastocyst TransferObstetrics and GynecologyComputational BiologyEmbryoEmbryo cultureEpithelial CellsCoculture Techniquesmedicine.anatomical_structureBlastocystReproductive MedicineCulture Media Conditionedembryonic structuresGestationFemaleBiomarkersFertility and sterility
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