Search results for "Protocol"
showing 10 items of 1808 documents
Antitumor Effects of a Combined 5-Aza-2′Deoxycytidine and Valproic Acid Treatment on Rhabdomyosarcoma and Medulloblastoma in Ptch Mutant Mice
2009
Abstract Patched (Ptch) heterozygous mice develop medulloblastoma (MB) and rhabdomyosarcoma (RMS) resembling the corresponding human tumors. We have previously shown that epigenetic silencing of the intact Ptch allele contributes to tumor formation in this model. Here, we investigated whether targeting of epigenetic silencing mechanisms could be useful in the treatment of Ptch-associated cancers. A reduction of endogenous DNA methyltransferase1 (Dnmt1) activity significantly reduced tumor incidence in heterozygous Ptch knockout mice. A combined treatment with the Dnmt inhibitor 5-aza-2′deoxycytidine (5-aza-dC) and the histone deacetlyase (HDAC) inhibitor valproic acid (VPA) efficiently prev…
Small molecule DNA-PK inhibitors as potential cancer therapy: a patent review (2010–present)
2021
Introduction: DNA-dependent protein kinase (DNA-PK) plays a crucial role in the repair of DSBs via non-homologous end joining (NHEJ). Several DNA-PK inhibitors are being investigated for potential anticancer treatment in clinical trials.Area covered: This review aims to give an overview of patents published since 2010 by analyzing the patent space and structure features of scaffolds used in those patents. It also discusses the recent clinical developments and provides perspectives on future challenges and directions in this field.Expert opinion: As a key component of the DNA damage response (DDR) pathway, DNA-PK appears to be a viable drug target for anticancer therapy. The clinical investi…
The molecular characterization of a depurinated trial DNA sample can be a model to understand the reliability of the results in forensic genetics
2014
The role of DNA damage in PCR processivity/fidelity is a relevant topic in molecular investigation of aged/forensic samples. In order to reproduce one of the most common lesions occurring in postmortem tissues, a new protocol based on aqueous hydrolysis of the DNA was developed in vitro. Twenty-five forensic laboratories were then provided with 3.0 μg of a trial sample (TS) exhibiting, in mean, the loss of 1 base of 20, and a molecular weight below 300 bp. Each participating laboratory could freely choose any combination of methods, leading to the quantification and to the definition of the STR profile of the TS, through the documentation of each step of the analytical approaches selected. …
DNS Tunneling Detection Techniques – Classification, and Theoretical Comparison in Case of a Real APT Campaign
2017
Domain Name System (DNS) plays an important role as a translation protocol in everyday use of the Internet. The purpose of DNS is to translate domain names into IP addresses and vice versa. However, its simple architecture can easily be misused for malicious activities. One huge security threat concerning DNS is tunneling, which helps attackers bypass the security systems unnoticed. A DNS tunnel can be used for three purposes: as a command and control channel, for data exfiltration or even for tunneling another protocol through it. In this paper, we surveyed different techniques for DNS tunneling detection. We classified those first based on the type of data and then within the categories b…
Las bibliotecas privadas en los protocolos notariales. Valencia 1780-1808
1984
El trabajo se ha realizado con protocolos notariales de la ciudad de Valencia, entre los años 1740 y 1808, sobre 1 304 inventarios postmortem, incluyendo en ellos algunas divisiones de bienes que llevaban previo el inventario. La mayor parte de los libros de protocolos utilizados se encuentran en el Archivo de Protocolos del Colegio de Corpus Christi de los que se han visto la casi totalidad de escribanos de esas fechas; en menor medida se han utilizado libros del Archivo del Reino de Valencia, en este archivo la selección de notarios ha sido absolutamente aleatoria, salvo en el caso del notario Joaquín Pastor, 1742-1797, del que se tenía noticia de su relación con el grupo mayansiano. En c…
#EEGManyLabs
2021
There is growing awareness across the neuroscience community that the replicability of findings about the relationship between brain activity and cognitive phenomena can be improved by conducting studies with high statistical power that adhere to well-defined and standardised analysis pipelines. Inspired by recent efforts from the psychological sciences, and with the desire to examine some of the foundational findings using electroencephalog-raphy (EEG), we have launched #EEGManyLabs, a large-scale international collaborative replication effort. Since its discovery in the early 20th century, EEG has had a profound in-fluence on our understanding of human cognition, but there is limited evid…
Real-World Data Analysis of Pregnancy-Associated Breast Cancer at a Tertiary-Level Hospital in Romania
2020
Background and objectives: Breast cancer is among the most common cancer types encountered during pregnancy. Here, we aimed to describe the characteristics, management, and outcomes of women with pregnancy-associated breast cancer at a tertiary-level hospital in Romania. Material and Methods: We retrospectively and prospectively collected demographic, oncological, and obstetrical data for women diagnosed with cancer during pregnancy, and who elected to continue their pregnancy, between June 2012 and June 2020. Complete data were obtained regarding family and personal medical history and risks factors, cancer diagnosis and staging, clinical and pathological features (including histology and …
The think aloud method in studying the translation process
2019
This paper presents a theoretical description of the process-oriented research method in translation studies known as the think aloud method and a case study of an experimental application of think-aloud protocols (TAP). The article begins with a brief description of the relevance of its topic, taking into account the state of current researches in the field of translation studies. A literature review of recent publications where the think aloud method and think-aloud protocols as the principal introspective and retrospective method in different translation studies is given. The shortcomings and advantages of applying the think aloud method are discussed and the ways to optimise the use of …
Technology-Supported Guidance Models Stimulating the Development of Critical Thinking in Clinical Practice: Protocol for a Mixed Methods Systematic R…
2020
BackgroundCritical thinking is an essential skill that nursing students need to develop. Technological tools have opened new avenues for technology-supported guidance models, but the challenges and facilitators of such guidance models, as well as how they stimulate the development of critical thinking, remain unclear.ObjectiveWe developed a protocol for a mixed methods systematic review to investigate the use of technology-supported guidance models that stimulate the development of critical thinking in nursing education clinical practice.MethodsA convergent integrated design following the Joanna Briggs Institute Manual for Evidence Synthesis will be employed. A pair of authors will select t…
A Distributed approach to the Remote Control and Programming Improvement
2006
The remote control of industrial plants through networks is becoming very important nowadays but it involves several issues that can degrade the performance and usability of the remote plant. This paper presents a distributed architecture improving a remote programming system which allows users to control and to program a plant remotely. The control system is based on a client/server architecture with many user interfaces that control the plant. The remote programming language is a mix of Java and client/server protocol in order to simplify the developing and to avoid the protocol/language translation. In order to obtain more efficiency, the program is developed in the client side and it is…