Search results for "Protéomique"
showing 9 items of 9 documents
Biominéralisation chez les Céphalopodes (Mollusca) : processus moléculaires et évolution
2021
Molluscs constitute one of the most diversified phyla within metazoans, known for their ability to mineralize a shell. The shell biomineralization is a genetically controlled process that is performed extracellularly via a calcifying organic matrix. This latter, which remains finally occluded (at least, partially) into the shell, is a mixture of proteins, glycoproteins, lipids and polysaccharides, that are collectively secreted by the external calcifying epithelium of the mantle. Today, the shell matrix is usually considered as the ‘molecular toolbox’ for constructing the shell. Since its discovery, it has been the focus of numerous studies, but mainly on bivalves and gastropods leaving cep…
Highlighting the role of diversity in driving weed dynamics and weed-crop interactions
2017
SPEBIOME INRA; Le Microscope à Dissection Laser (MDL) est composé de l’association d’un microscope équipé d’objectifs permettant un grossissement x2 à x100 et d’un système optique de découpe laser. Il permet de microdisséquer de façon ciblée des échantillons biologiques, de type bactérien, animal ou végétal. Sa finesse de dissection permet de sélectionner, d’isoler puis de récupérer des cellules cibles afin de concentrer le matériel. Ainsi, il est possible de réaliser des analyses de transcriptomique, de génomique ou de protéomique à partir de cellules cibles. Avant de microdisséquer, la préparation des échantillons est primordiale pour s’assurer de récupérer une qualité et une quantité suf…
La métaprotéomique pour l'analyse fonctionnelle des communautés bactéreinnes indigènes
2007
Metaproteomics: a new approach for studying functional microbial ecology
2008
Establishing the link between microbial diversity and ecosystem functioning constitutes one of the fundamental questions in microbial ecology. However, microbial biodiversity cannot be resolved in a single manner and the question arises about which of the genetic and/or taxonomic and/or functional components of biodiversity are the most relevant in such a problematic. To date, most researches in this field has focused on genetic diversity and few investigations have examined functional diversity, resulting in a poor understanding of the manner in which genetic and taxonomic diversity affect functional diversity or ecosystem properties. This can be explained by paucity of methods allowing an…
Technical improvements for analysis of recalcitrant proteins by LC-MS : the myccorhiza responsive membrane proteome as a case study
2012
Arbuscular mycorrhizas (AM) are widespread symbiotic associations between plant roots and AM fungi. Deep membrane alterations are the foremost morphological changes occurring in the host plant in response to AM symbiosis. Two-dimensional gel electrophoresis (2-DE) is the workhorse method in AM proteomics. Membrane proteins are under-represented in 2-DE because of their hydrophobicity, low abundance, and precipitation at their isoelectric point, thereby few are the identified membrane proteins involved in sustaining the AM symbiosis. Membrane proteomics is still challenging due to 2-DE related shortcomings, however latest trends and advancements in mass spectrometry (MS)-based quantitative p…
Identification des fonctions microbiennes associées à la dégradation du soufre organique dans les sols par une approche de métaprotéomique
2009
National audience
Technical improvements for analysis of recalcitrant proteins by LC-MS : the mycorrhiza responsive membrane proteome as a case study
2012
Arbuscular mycorrhizas (AM) are widespread symbiotic associations between plant roots and AM fungi. Deep membrane alterations are the foremost morphological changes occurring in the host plant in response to AM symbiosis. Two-dimensional gel electrophoresis (2-DE) is the workhorse method in AM proteomics. Membrane proteins are under-represented in 2-DE because of their hydrophobicity, low abundance, and precipitation at their isoelectric point, thereby few are the identified membrane proteins involved in sustaining the AM symbiosis. Membrane proteomics is still challenging due to 2-DE related shortcomings, however latest trends and advancements in mass spectrometry (MS)-based quantitative p…
La "Paléoshellomique" dans les starting-blocks
2021
Systèmes d'Information Scientifique : des modèles conceptuels aux annotations sémantiques Application au domaine de l'archéologie et des sciences du …
2013
Les Systèmes d'Information Scientifique (SIS) sont des Systèmes d'Information (SI) dont le but est de produire de la connaissance et non pas de gérer ou contrôler une activité de production de biens ou de services comme les SI d'entreprise. Les SIS se caractérisent par des domaines de recherche fortement collaboratifs impliquant des équipes pluridisciplinaires et le plus souvent géographiquement éloignées, ils manipulent des données aux structures très variables dans le temps qui vont au-delà de la simple hétérogénéité : nuages de points issus de scanner 3D, modèles numériques de terrain, cartographie, publications, données issues de spectromètre de masse ou de technique de thermoluminescen…