Search results for "Pseudomonas putida"
showing 7 items of 17 documents
Antimikrobiālo virsmu efektivitāte atkarībā no mikroorganismu fizioloģiskā stāvokļa
2020
Esošā pētījuma uzdevums ir salīdzināt vara nanodaļiņu antimikrobiālā pārklājuma iedarbības efektivitāti uz divām baktēriju sugām, to trijos fizioloģiskajos stāvokļos, kā arī novērtēt izmantotās metodoloģijas piemērotību mērķa sasniegšanai. Mērķa sasniegšanai testēja vara nanodaļiņu antimikrobiālā pārklājuma ietekmi uz Pseudomonas putida un Staphylococcus epidermidis kultūrām, to eksponenciālās augšanas fāzē, stacionārajā fāzē un uz kultūrām, kuras tika audzētas uz agarizētās barotnes. Testēšanas metodoloģija tika iepriekš aprobēta LU MBI organizētā AMiCi (CA15114 – Anti-Microbial Coating Innovation to prevent infectious diseases) Training School “Assessment of the effecticity of antimicrobi…
Production of a Recombinant Catechol 2,3-Dioxygenase for the Degradation of Micropollutants
2017
Phenolic compounds such as catechol represent a particular type of micropollutant whose high stability prevents rapid decay and metabolization in the environment. We successfully cloned a catechol 2,3-dioxygenase (C2,3O) from Pseudomonas putida mt-2 and expressed it in Escherichia coli BER2566. The biomass isolated from shake-flask fermentations was used to partially purify the enzyme. The enzyme proved unstable in clarified liquid fractions (50 mM Tris buffer, pH 7.6) and lost more than 90% of its activity over 7 h at 25 °C. In the presence of 10% acetone, the process was slowed down and 30% residual activity was still present after 7 h incubation. Storage of the enzyme in clear liquid fr…
Comparison of inoculums in the removal of 2-butoxyethanol from air emissions by biotrickling filter: Performance and microbial monitoring
2014
2-butoxyethanol is one of the most used glycol ether in industrial activities and the treatment of air 2-butoxyethanol-emissions become necessary. Biotechnologies are potential treatment technologies due to their low operational costs. The use of two inoculums in the treatment of 2-butoxyethanol by biotrickling filters (BTFs) packed with polyurethane-foam was studied. A pure culture of Pseudomonas putida, previously adapted to 2-butoxyethanol, was used as inocula in a BTF operated in the University of Stuttgart. Fresh activated sludge from a municipal waste water treatment plant was used as inocula in a BTF operated in the University of Valencia. An empty bed residence time of 12.5 s and in…
Numerical Taxonomy of Pseudomonads Isolated from Water, Sediment and Eels
1992
Summary A numerical taxonomic study was performed on 129 pseudomonad isolates from aquatic environments. 67 out of 98 field strains were isolated from water and sediment samples from Albufera lake, a hypereutrophic lake near Valencia, and 31 strains came from an eel hatchery. 10 clusters were formed at 84% similarity level when the Sokal-Michener similarity coefficient was used. Five clusters were designated as Pseudomonas putida (16 strains), P. fluorescens biotype I (9 strains), P. pseudoalcaligenes (11 strains), P. stutzen (4 strains) and Shewanella putrefaciens (3 strains). A subcluster of 7 strains (out of 15) was ascribed to the species Xanthomonas maltophilia , while the other strain…
Isolation and characterisation of a bacterial strain degrading the herbicide sulcotrione from an agricultural soil
2011
BACKGROUND: The dissipation kinetics of the herbicide sulcotrione sprayed 4 times on a French soil was studied using a laboratory microcosm approach. An advanced cultivation-based method was then used to isolate the bacteria responsible for biotransformation of sulcotrione. Chromatographic methods were employed as complementary tools to define its metabolic pathway. RESULTS: Soil microflora was able quickly to biotransform the herbicide (DT50 ≈ 8 days). 2-Chloro-4-mesylbenzoic acid, one of its main metabolites, was clearly detected. However, no accelerated biodegradation process was observed. Eight pure sulcotrione-resistant strains were isolated, but only one (1OP) was capable of degrading…
Data from: Evolution of bacterial life history traits is sensitive to community structure
2016
Very few studies have experimentally assessed the evolutionary effects of species interactions within the same trophic level. Here we show that when Serratia marcescens evolve in multispecies communities, their growth rate exceeds the growth rate of the bacteria that evolved alone, whereas the biomass yield gets lower. In addition to the community effects per se, we found that few species in the communities caused strong effects on S. marcescens evolution. The results indicate that evolutionary responses (of a focal species) are different in communities, compared to species evolving alone. Moreover, selection can lead to very different outcomes depending on the community structure. Such con…
Data from: Propagule pressure increase and phylogenetic diversity decrease community’s susceptibility to invasion
2017
Invasions pose a large threat to native species, but the question of why some species are more invasive, and some communities more prone to invasions than others, is far from solved. Using ten different three-species bacterial communities, we tested experimentally if the phylogenetic relationships between an invader and a resident community and propagule pressure affect invasion probability. We found that greater diversity in phylogenetic distances between the resident community members and the invader lowered invasion success, and higher propagule pressure increased invasion success whereas phylogenetic distance had no clear effect. In the later stages of invasion phylogenetic diversity ha…