Search results for "Pulse"

showing 10 items of 1508 documents

mcr-1- like detection in commensal Escherichia coli and Salmonella spp. from food-producing animals at slaughter in Europe

2017

International audience; We evaluate here the presence of the mcr-1-like and mcr-2 genes in Escherichia coli and Salmonella spp. isolated from healthy food-producing animals at slaughter between 2002 and 2014 in Europe. Isolates were retrieved from cattle, pig and chicken from 11 European countries of production. The susceptibility to colistin and antibiotics used in human medicine was determined by agar dilution. Colistin-resistant isolates were PCR-screened for mcr genes. mcr-positive isolates were typed by Pulsed-Field Gel Electrophoresis (PFGE) and Multi-Locus Sequence Typing. Among the 10,206 E. coli and 1774 Salmonella spp. isolated from cattle, pigs and chickens, 148 E. coli and 92 Sa…

0301 basic medicineSalmonellaVeterinary medicineFood-producing animalsmedicine.drug_classSwine030106 microbiologyAntibioticsBiology[ SDV.MP.BAC ] Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriologymedicine.disease_causeMicrobiologyAgar dilution03 medical and health sciencesBacterial Proteins[ SDV.MP ] Life Sciences [q-bio]/Microbiology and ParasitologySalmonellaDrug Resistance BacterialmedicinePulsed-field gel electrophoresisEscherichia coliAnimalsTypingEscherichia coliEscherichia coli Infections2. Zero hungerSalmonella Infections AnimalGeneral VeterinaryColistinEscherichia coli ProteinsGeneral Medicine[SDV.MP.BAC]Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology3. Good healthAnti-Bacterial AgentsBacterial Typing TechniquesElectrophoresis Gel Pulsed-FieldEuropeSalmonella spp.ColistinMCR-1CattleChickensMCR-1Abattoirshormones hormone substitutes and hormone antagonistsmedicine.drugMultilocus Sequence Typing
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Diversity, virulence, and antimicrobial resistance of the KPC-producing Klebsiella pneumoniae ST307 clone

2017

ABSTRACT : The global spread of Klebsiella pneumoniae producing Klebsiella pneumoniae carbapenemase (KPC) has been mainly associated with the dissemination of high-risk clones. In the last decade, hospital outbreaks involving KPC-producing K. pneumoniae have been predominantly attributed to isolates belonging to clonal group (CG) 258. However, results of recent epidemiological analysis indicate that KPC-producing sequence type (ST) 307, is emerging in different parts of the world and is a candidate to become a prevalent high-risk clone in the near future. Here we show that the ST307 genome encodes genetic features that may provide an advantage in adaptation to the hospital environment and t…

0301 basic medicineSettore MED/07 - Microbiologia E Microbiologia Clinicasiderophoreantibiotic resistancelong term survivalsequence analysisKlebsiella pneumoniaepolymerase chain reactionResponses to Human InterventionsDrug ResistanceGene TransferClone (cell biology)ST259bacterial proteinvirulence factorYersiniabactinGenomechemistry.chemical_compoundMicrobialPlasmidAntibioticsbacterial genomepathogenicitygenetics610 Medicine & healthgenome analysisCross InfectionMolecular EpidemiologyGenomeVirulencebiologydrug effectyersiniabactinBacterialDrug Resistance MicrobialGeneral MedicineKlebsiella infectionglycogen synthesisKlebsiella pneumoniaeEnglandItalyST307horizontal gene transferProteínas BacterianasResearch ArticleGene Transfer HorizontalVirulence FactorsSequence analysiscapsule030106 microbiologyVirulence610 Medicine & healthpulsed field gel electrophoresisColombiaCarbapenemase; siderophore; yersiniabactin; bacterial protein; beta lactamase; virulence factor antibiotic resistance; Article; bacterial strain; bacterial virulence; bacterium isolate; fimbria; genome analysis; glycogen synthesis; Klebsiella pneumoniae; long term survival; microbial diversity; nonhuman; plasmid; polymerase chain reaction; pulsed field gel electrophoresis; sequence analysis; whole genome sequencing; antibiotic resistance; bacterial genome; carbapenem-resistant Enterobacteriaceae; Colombia; cross infection; drug effect; England; genetic variation; genetics; horizontal gene transfer; human; Italy; Klebsiella infection; microbiology; molecular epidemiology; multilocus sequence typing; pathogenicity; virulence Bacterial Proteins; beta-Lactamases; Carbapenem-Resistant Enterobacteriaceae; Colombia; Cross Infection; Drug Resistance Microbial; England; Gene Transfer Horizontal; Genetic Variation; Genome Bacterial; Humans; Italy; Klebsiella Infections; Klebsiella pneumoniae; Molecular Epidemiology; Multilocus Sequence Typing; Virulence; Virulence Factors; Whole Genome SequencingArticlebeta-Lactamasesbeta lactamaseHorizontalMicrobiologyCarbapenemase03 medical and health sciencesAntibiotic resistanceBacterial ProteinsplasmidHumanshumanInfecciones por KlebsiellafimbrianonhumanWhole Genome Sequencingbacterial virulencebacterium isolatemicrobiologyGenetic Variationbacterial strainbiology.organism_classificationKlebsiella InfectionsEnterobacteriaceae Resistentes a los CarbapenémicosKPCCarbapenem-Resistant Enterobacteriaceae030104 developmental biologychemistrymicrobial diversityEpidemiología MolecularGenome BacterialWGSMultilocus Sequence Typing
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Adherence to recommended intake of pulses and related factors in university students in the UniHcos project

2020

This study was supported by the National Drug Plan, Ministry of Health, Social Services and Equality of Spain (Grants: 2010|145 and 2013|034).

0301 basic medicineSociodemographic FactorsUniversitiesMedicine (miscellaneous)Social WelfareNutrition Policy03 medical and health sciences0302 clinical medicineEnvironmental healthMedicineHumans030212 general & internal medicineProspective StudiesStudentsPulsesNutritionRelated factors030109 nutrition & dieteticsNutrition and Dieteticsbusiness.industryFeeding BehaviorLegumesDietUniversity studentsCross-Sectional StudiesDiet qualitySpainMedicina Preventiva y Salud PúblicaChristian ministrybusinessEdible GrainDiet qualityRecommended Intake
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Antibiotic Resistance Profiling, Analysis of Virulence Aspects and Molecular Genotyping of Staphylococcus aureus Isolated in Sicily, Italy

2018

Abstract Staphylococcus aureus is the major cause of foodborne diseases worldwide. In this retrospective study, 84 S. aureus strains were characterized. The collection comprises 78 strains isolated during 1998 and 2014 from dairy products and tissue samples from livestock bred for dairy production in Sicily. One isolate was obtained from a pet (dog), one from an exotic animal (a circus elephant), and four human isolates were obtained during a severe food poisoning outbreak that occurred in Sicily in 2015. All the strains were characterized by pulsed-field gel electrophoresis (PFGE), for antibiotic resistance and presence of toxin genes. PFGE results showed 10 different pulsotypes, with thre…

0301 basic medicineStaphylococcus aureusLivestockantibiotic resistanceGenotypeMLST; MRSA; PFGE; Staphylococcus aureus; antibiotic resistance; toxin genesTetracycline030106 microbiologyVirulenceMRSABiologymedicine.disease_causeSettore BIO/19 - Microbiologia GeneraleApplied Microbiology and BiotechnologyMicrobiologyMicrobiologyFoodborne DiseasesEnterotoxins03 medical and health sciencesAntibiotic resistanceDrug Resistance BacterialPulsed-field gel electrophoresismedicineAnimalsHumansSicilyRetrospective StudiesVirulenceOutbreakOriginal ArticlesPFGEStaphylococcal InfectionsAnti-Bacterial AgentsBacterial Typing TechniquesElectrophoresis Gel Pulsed-FieldPenicillin030104 developmental biologyStaphylococcus aureustoxin genesStaphylococcus aureuFood MicrobiologyMultilocus sequence typingAnimal Science and ZoologyMultilocus Sequence TypingMLSTFood Sciencemedicine.drug
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A Stage-Based Approach to Therapy in Parkinson’s Disease

2019

Parkinson’s disease (PD) is a neurodegenerative disorder that features progressive, disabling motor symptoms, such as bradykinesia, rigidity, and resting tremor. Nevertheless, some non-motor symptoms, including depression, REM sleep behavior disorder, and olfactive impairment, are even earlier features of PD. At later stages, apathy, impulse control disorder, neuropsychiatric disturbances, and cognitive impairment can present, and they often become a heavy burden for both patients and caregivers. Indeed, PD increasingly compromises activities of daily life, even though a high variability in clinical presentation can be observed among people affected. Nowadays, symptomatic drugs and non-phar…

0301 basic medicinemedicine.medical_specialtyParkinson's diseaseImpulse control disorderlcsh:QR1-502ReviewDiseasel-dopaBiochemistryREM sleep behavior disorderMotor symptomslcsh:MicrobiologyAntiparkinson Agentsnon-pharmacological therapy03 medical and health sciences0302 clinical medicinePhysical medicine and rehabilitationmedicineHumansdopamine-agonistsApathyanticholinergicsResting tremorMolecular Biologyamantadinebusiness.industryAmantadineParkinson Diseasemonoamine oxidase inhibitorsmotor symptomsmedicine.diseasenon-motor symptoms030104 developmental biologyacetylcholinesterase inhibitorsParkinson’s disease<span style="font-variant: small-caps">l</span>-dopaSettore MED/26 - Neurologiamedicine.symptombusiness030217 neurology & neurosurgerymedicine.drugBiomolecules
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Higher glucose availability augments the metabolic responses of the C2C12 myotubes to exercise-like electrical pulse stimulation

2021

The application of exercise-like electrical pulse simulation (EL-EPS) has become a widely used exercise mimetic in vitro. EL-EPS produces similar physiological responses as in vivo exercise, while less is known about the detailed metabolic effects. Routinely, the C2C12 myotubes are cultured in high-glucose medium (4.5 g/L), which may alter EL-EPS responses. In this study, we evaluate the metabolic effects of EL-EPS under the high- and low-glucose (1.0 g/L) conditions to understand how substrate availability affects the myotube response to EL-EPS. The C2C12 myotube, media, and cell-free media metabolites were analyzed using untargeted nuclear magnetic resonance (NMR)-based metabolomics. Furt…

0301 basic medicinemedicine.medical_specialtyasetaatitbranched chain fatty acidsPhysiologyEndocrinology Diabetes and MetabolismMuscle Fibers SkeletalrasvahapotStimulationglukoosi03 medical and health sciencesMice0302 clinical medicineMetabolomicsPhysiology (medical)Internal medicinePhysical Conditioning AnimalMetabolomemedicineAnimalsskeletal muscleaineenvaihduntalihassolutCells CulturedsolufysiologiaChemistryPulse (signal processing)MyogenesisSkeletal muscleBranched chain fatty acidsmetabolomicslaktaatitElectric Stimulation030104 developmental biologymedicine.anatomical_structureEndocrinologyGlucosein vitro -menetelmäaineenvaihduntatuotteetacetateexerkineC2C12030217 neurology & neurosurgeryAmino Acids Branched-ChainResearch Article
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The Importance of Developing Electrochemical Sensors Based on Molecularly Imprinted Polymers for a Rapid Detection of Antioxidants

2021

International audience; This review aims to pin out the importance of developing a technique for rapid detection of antioxidants, based on molecular imprinting techniques. It covers three major areas that have made great progress over the years in the field of research, namely: antioxidants characterization, molecular imprinting and electrochemistry, alone or combined. It also reveals the importance of bringing these three areas together for a good evaluation of antioxidants in a simple or complex medium, based on selectivity and specificity. Although numerous studies have associated antioxidants with molecular imprinting, or antioxidants with electrochemistry, but even electrochemistry wit…

0301 basic medicinesol-gel techniquePhysiologyClinical Biochemistryelectrochemical sensorNanotechnologyReviewBiochemistryRapid detection03 medical and health sciences0302 clinical medicine[SDV.IDA]Life Sciences [q-bio]/Food engineering[SDV.BBM]Life Sciences [q-bio]/Biochemistry Molecular BiologyMolecular Biologyscreen printed electrodesChemistrylcsh:RM1-950Molecularly imprinted polymerCell Biologyradical polymerizationcyclic voltammetrylcsh:Therapeutics. Pharmacologyantioxidants030104 developmental biologyindustrial applicationsmolecular imprintingMolecular imprinting030217 neurology & neurosurgerydifferential pulse voltammetryAntioxidants
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AB0691 INCREASED CARDIOVASCULAR RISK IN MIXED CONNECTIVE TISSUE DISEASE: EVALUATION OF MACROVASCULAR INVOLVEMENT AND ITS PREDICTORS BY AORTIC PULSE W…

2019

Background: Macrovascular involvement and cardiovascular (CV) risk have not been sufficiently studied in patients with mixed connective tissue disease (MCTD). In particular, the gold standard assessment method of aortic stiffness carotid-femoral pulse wave velocity (cfPWV) (1) has never been evaluated in patients with this disease. Objectives: Aims of the present study were to examine cfPWV in MCTD and to evaluate its associations with MCTD associated parameters and traditional CV risk factors. Methods: cfPWV measurements were performed in 43 MCTD patients and 107 healthy controls. The difference between cfPWV in the two groups was statistically examined and subsequently controlled for the …

030203 arthritis & rheumatology0301 basic medicinemedicine.medical_specialtybusiness.industryGold standardConfoundingmedicine.disease03 medical and health sciences030104 developmental biology0302 clinical medicineMixed connective tissue diseaseBlood pressureInternal medicineAssessment methodsmedicineCardiologyAortic stiffnessIn patientbusinessPulse wave velocityAbstracts accepted for Publication
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Pulsed electromagnetic field therapy in the treatment of pain and other symptoms in fibromyalgia: A randomized controlled study

2018

Low-energy pulsed electromagnetic field (PEMF) therapy has been suggested as a promising therapy to increase microcirculation, which is of great concern in patients with fibromyalgia. This study evaluated the effectiveness of PEMF therapy on the treatment of fibromyalgia. A group of 108 women with fibromyalgia were allocated to a 12-week treatment period with an active Bio-Electro-Magnetic-Energy-Regulation (BEMER) device and a similar treatment period with an inactive device. Each patient received active and sham treatments in a random order. Pain and stiffness were assessed on a visual analog scale (VAS, scale 0-100 mm), and functional status was assessed by the Fibromyalgia Impact Questi…

030203 arthritis & rheumatologyPhysiologybusiness.industryVisual analogue scaleBiophysicsGeneral Medicinemedicine.diseaseTreatment periodlaw.invention03 medical and health sciences0302 clinical medicineRandomized controlled triallawAnesthesiaFibromyalgiaMedicineRadiology Nuclear Medicine and imagingFunctional statusActive treatmentbusinessPulsed electromagnetic field therapy030217 neurology & neurosurgeryAfter treatmentBioelectromagnetics
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THU0520 Assessment of cardiovascular risk in patients with fibromyalgia by carotid-femoral pulse wave velocity – results of a prospective study

2018

Background Autonomic dysfunction, a basic element of fibromyalgia (FM), has been in some cases related to increased risk of cardiovascular (CV) disease. CV risk associates with aortic stiffness, which can be reliably assessed by carotid-femoral pulse wave velocity (cfPWV). Objectives Aims of this study were to test the hypothesis of increased cfPWV in a group of patients with FM and to examine its association with FM associated parameters and selected traditional CV risk factors. Methods We performed measurements of cfPWV in 99 FM patients and 102 healthy controls. The difference between cfPWV values in the two groups after controlling for possible confounding factors was evaluated through …

030203 arthritis & rheumatologymedicine.medical_specialtyVisual analogue scalebusiness.industryConfoundingRegression analysisGold standard (test)medicine.disease03 medical and health sciences0302 clinical medicineFibromyalgiaInternal medicinemedicineCardiologyAortic stiffness030212 general & internal medicineProspective cohort studybusinessPulse wave velocityTHURSDAY, 14 JUNE 2018
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