Search results for "Pulsed"

Blattabacteria, the endosymbionts of cockroaches, have small genome sizes and high genome copy numbers.

2008

Summary Blattabacteria are intracellular endosymbionts of cockroaches and primitive termites that belong to the class Flavobacteria and live only in specialized cells in the abdominal fat body of their hosts. In the present study we determined genome sizes as well as genome copy numbers for the endosymbionts of three cockroach species, Blattella germanica, Periplaneta americana and Blatta orientalis. The sole presence of blattabacteria in the fat body was demonstrated by rRNA-targeting techniques. The genome sizes of the three blattabacteria were determined by pulsed field gel electrophoresis. The resulting total genome sizes for the three symbionts were all approximately 650 15 kb. Compari…

Microbiological quality of carbonated drinking water produced with in-home carbonation systems.

2005

The microbiological quality of carbonated water produced with tap water in commercial in-home carbonation systems was determined, the results being discussed in the context of the microbiological quality of the tap water used, the properties of the drink makers, and the procedures of preparation and washing of various parts of the appliance. The last-mentioned data were received from each participant of the study by questionnaire. Escherichia coli, coliforms, fecal streptococci and spore-forming sulphite-reducing anaerobes were used as indicators for the hygienic quality of the water. Tap-water samples were collected according to the usual procedure when filling the carbonating bottle, i.e.…

Characterisation of a Cryptosporidium parvum-specific cDNA clone and detection of parasite DNA in mucosal scrapings of infected mice.

1998

A cDNA library was constructed using total RNA extracted from oocysts and sporozoites of the protozoan parasite Cryptosporidium parvum. The expression library was screened with an anti-C. parvum antiserum and a clone, Cp3.4, with a 2043 bp insert, was extracted. Southern blot analysis demonstrated a single copy gene that was located on a 1.6 Mb chromosome. The gene was found to be C. parvum specific as Cp3.4 did not cross-hybridise with chromosomal DNA from three other apicomplexan parasites. The cDNA encodes a polypeptide with a predicted membrane helix at its C-terminal end which is flanked by stretches of acidic amino acids. Overall, the polypeptide has a low isoelectric point (pI) of 3.…

Pulsed EPR determination of water accessibility to spin-labeled amino acid residues in LHCIIb.

2009

Membrane proteins reside in a structured environment in which some of their residues are accessible to water, some are in contact with alkyl chains of lipid molecules, and some are buried in the protein. Water accessibility of residues may change during folding or function-related structural dynamics. Several techniques based on the combination of pulsed electron paramagnetic resonance (EPR) with site-directed spin labeling can be used to quantify such water accessibility. Accessibility parameters for different residues in major plant light-harvesting complex IIb are determined by electron spin echo envelope modulation spectroscopy in the presence of deuterated water, deuterium contrast in …

Contact and contactless diffuse reflectance spectroscopy: potential for recovery monitoring of vascular lesions after intense pulsed light treatment.

2011

Optical fiber contact probe diffuse reflectance spectroscopy and remote multispectral imaging methods in the spectral range of 400 to 1100 nm were used for skin vascular malformation assessment and recovery tracing after treatment by intense pulsed light. The results confirmed that oxy-hemoglobin relative changes and the optical density difference between lesion and healthy skin in the spectral region 500 to 600 nm may be successfully used for objective appraisal of the therapy effect. Color redness parameter a* = 2 is suggested as a diagnostic border to distinguish healthy skin and vascular lesions, and as the indicator of phototreatment efficiency. Valuable diagnostic information on large…

Determination of New Radical Species in Ammonium Tartrate Dosimeters by CW- and Pulsed-EPR Techniques

2015

Samples of ammonium tartrate irradiated with doses of about 0.1–1 kGy by different beams of ionizing radiation (60Co–γ, 19 MeV protons and 62 MeV per nucleon carbon ions) were studied by continuous-wave electron paramagnetic resonance (cw-EPR) and by pulse-EPR techniques. Careful analysis of the cw-EPR and of the echo-detected EPR spectra allowed the identification of a second radical in the system besides the already known radical formed at high temperature by an hydrogen elimination at C(2) position [M. Brustolon et al., Res. Chem. Int 4:359, 1996]. The spectrum of the radical is compatible with that of a radical obtained by hydroxyl elimination.

Pulsed EPR investigations on radiations dosimeters.

2009

FOCAL LIVER LESIONS: CONTRAST-ENHANCED ULTRASOUND

2008

Contrast-enhanced ultrasound (CEUS) represents a significant breakthrough in sonography and it is being increasingly used for evaluation of focal liver lesions (FLLs). The unique feature of CEUS of non-invasively assessing in real time liver perfusion throughout the vascular phase has led to a dramatic improvement in diagnostic accuracy of US in either detection and characterization of FLLs, as well as in the guidance and evaluation of response of therapeutic procedures. Currently, CEUS is included as a part of the suggested diagnostic work-up of FLLs, resulting in a better patient management and cost-effective therapy delivering. After a brief description of the basis of different CEUS tec…

Electrochemical methods for carrier type identification of ZnO films grown by pulsed laser deposition on InP.

2013

Experimental investigation of low-frequency pulsed Lorentz force influence on the motion of Galinstan melt

2016

Abstract The paper presents the results of the numerical and physical experiments, aimed at assessing the influence of pulsed force of electromagnetic field on the melt motion and the fluid velocities. The experiment was performed on the eutectic alloy Galinstan in the cylindrical volume, where an ultrasonic Doppler velocimeter was employed for velocity measurements under conditions of pulsed and steady EM field application. A numerical simulation of the melt flow, forced by the steady EM force, involved a 2D axisymmetric model. The k-e turbulence model was used to obtain the information about the melt velocities. The verification of the numerical model was carried out for the steady case. …