Search results for "QUANTIFICATION"

Abundance of NARG, NIRK and NOSZ genes of denirifying bacteria during primary successions of a glacier foreland

2007

[div]Long-term dynamics of the atrazine mineralization potential in surface and subsurface soil in an agricultural field as a response to atrazine ap…

2012

Abstract The dynamics of the atrazine mineralization potential in agricultural soil was studied in two soil layers (topsoil and at 35–45 cm depth) in a 3 years field trial to examine the long term response of atrazine mineralizing soil populations to atrazine application and intermittent periods without atrazine and the effect of manure treatment on those processes. In topsoil samples, 14 C-atrazine mineralization lag times decreased after atrazine application and increased with increasing time after atrazine application, suggesting that atrazine application resulted into the proliferation of atrazine mineralizing microbial populations which decayed when atrazine application stopped. Decay …

Erosion des sols du Cap Vert : processus et quantification à l'échelle de trois Bassins Versants de l'île de Santiago

2010

The archipelago of Cape Verde comprises 10 volcanic islands and belongs to the Sahelian zone stretching from the Atlantic to the Red Sea. Since several decades, Cape Verde is affected by desertification caused largely by climatic recession and soil erosion. These factors, coupled with high population pressure on resources, rugged topography and torrential tropical rains, cause serious losses of lands. However, since its independence in 1975, the Government conducted an extensive program of afforestation, soil and water conservation. Nevertheless very little research has been conducted to evaluate the actions of protection and conservation of soil and water. Therefore, there is no data on th…

Quantification of denitrifiers by real-time PCR

2007

Quantifying cereal-reaping microwear on flint tools : an experimental approach

2016

International audience; From the earliest Neolithic in the Near East to the last Chalcolithic cultures in Western Europe, certain flint tools have been used as sickles to harvest cereals. Such harvesting tools can be identified through use-wear analyses, because cutting herbaceous plants produces specific wear-traces on the working edge of flint blades. The aim of this work is to explore harvesting-driven microwear variability and, more particularly, intensity of use as a governing factor. To achieve this objective, an experiment was designed consisting in the production of flint replicas to be used as harvesting tools, in various controlled conditions. A simple, cost-effective method of qu…

Identification and quantification of microbial populations in activated sludge and anaerobic digestion processes

2014

Eight different phenotypes were studied in an activated sludge process (AeR) and anaerobic digester AnD) in a full-scale wastewater treatment plant by means of fluorescent in situ hybridization (FISH) and automated FISH quantification software. The phenotypes were ammonia-oxidizing bacteria, nitrite-oxidizing bacteria, denitrifying bacteria, phosphate-accumulating organisms (PAO), glycogen-accumulating organisms (GAO), sulphate-reducing bacteria (SRB), methanotrophic bacteria and methanogenic archaea. Some findings were unexpected: (a) Presence of PAO, GAO and denitrifiers in the AeR possibly due to unexpected environmental conditions caused by oxygen deficiencies or its ability to survive …

Multicenter Consistency Assessment of Valvular Flow Quantification With Automated Valve Tracking in 4D Flow CMR

2021

OBJECTIVES This study determined: 1) the interobserver agreement; 2) valvular flow variation; and 3) which variables independently predicted the variation of valvular flow quantification from 4-dimensional (4D) flow cardiac magnetic resonance (CMR) with automated retrospective valve tracking at multiple sites. BACKGROUND Automated retrospective valve tracking in 4D flow CMR allows consistent assessment of valvular flow through all intracardiac valves. However, due to the variance of CMR scanners and protocols, it remains uncertain if the published consistency holds for other clinical centers. METHODS Seven sites each retrospectively or prospectively selected 20 subjects who underwent whole …

The deubiquitinase USP11 is a versatile and conserved regulator of autophagy

2021

Autophagy is a major cellular quality control system responsible for the degradation of proteins and organelles in response to stress and damage to maintain homeostasis. Ubiquitination of autophagy-related proteins or regulatory components is important for the precise control of autophagy pathways. Here, we show that the deubiquitinase ubiquitin-specific protease 11 (USP11) restricts autophagy and that KO of USP11 in mammalian cells results in elevated autophagic flux. We also demonstrate that depletion of the USP11 homolog H34C03.2 in Caenorhabditis elegans triggers hyperactivation of autophagy and protects the animals against human amyloid-β peptide 42 aggregation-induced paralysis. USP11…

The origin, definition and nature of the transconscious in the spirituality of Father Stăniloae

2022

In his important work, Orthodox Spirituality , Father Dumitru Staniloae considers that, besides the real existence of the subconscious, there exists in man another reality as well, comprising the superior divine energies found in the human heart and which he defined by using the modern notion of transconscious or supraconscious. Contribution: The present study emphasises on the origin, definitions and content of this concept in the work of Father Staniloae and its great importance in defining a way to know God and his salvation works, from the perspective of an anchorite and Christian mystic, engaged in the existential tension of communion with God.

Estimation of the density of the protocatechuate-degrading bacterial community in soil by real-time PCR

2008

Summary The β-ketoadipate pathway is the major route for degradation of aromatic compounds by various soil microorganisms. Protocatechuate 3,4-dioxygenase, a key enzyme of this pathway and which is encoded by pcaGH genes, catalyses the ring cleavage of protocatechuate. Microorganisms harbouring pcaGH genes are widely distributed in the environment but little is known about their relative abundance within the total microflora. Hence, this paper reports the development of a real-time PCR assay to quantify the bacterial pcaH sequence that encodes the β sub-unit of the protocatechuate 3,4-dioxygenase. This real-time PCR assay was linear over seven orders of magnitude with a calculated efficienc…