Search results for "RADIS"
showing 10 items of 95 documents
Oxidative oligomerization of cyclodextrin-complexed bifunctional phenols catalyzed by horseradish peroxidase in water
2000
The HRP-catalyzed oligomerization of hydrophobic bifunctional phenols in water was realized by the use of 2,6-di-O-methylated β-cyclodextrin. The molecular weights of the resulting oligomers were in the same region as they were reached by conventional HRP-catalyzed oligomerizations in water/dioxane-mixtures. The polymerizable moieties, maleimide and methacrylamide, were not affected during the oligomerization process, as proofed by NMR, MALDI-TOF and FT-IR measurements. It was therefore possible to get soluble functionalized oligomers, which were crosslinked via radical copolymerization with suitable components (styrene, MMA) or heating.
Comparative effect of supercritical carbon dioxide and high pressure processing on structural changes and activity loss of oxidoreductive enzymes
2019
Abstract Due to the CO2 specific characteristics, it has been used as supercritical (Sc) fluid for several applications, including enzyme inactivation. The influence of Sc-CO2 (10–65 MPa/10–30 min/35–65 °C) on mushroom polyphenol oxidase (PPO) and horseradish peroxidase (POD) was evaluated and the results were compared with those found using high pressure processing (HPP) (200–900 MPa/5–45 °C/1–15 min). The free ion concentration was also studied to compare the enzymatic activity and changes in electrical conductivity. Additionally both enzymes, untreated or treated using either Sc-CO2 or HPP, were used as additives in the CuCl2 crystallization method. The resulting additive-specific CuCl2 …
Hydrogen peroxide sensing with horseradish peroxidase-modified polymer single conical nanochannels.
2011
Inspired from the funtioning and responsiveness of biological ion channels, researchers attempt to develop biosensing systems based on polymer and solid-state nanochannels. The applicability of these nanochannels for detection/sensing of any foreign analyte in the surrounding environment depends critically on the surface characteristics of the inner walls. Attaching recognition sites to the channel walls leads to the preparation of sensors targeted at a specific molecule. There are many nanochannel platforms for the detection of DNA and proteins, but only a few are capable of detecting small molecules. Here, we describe a nanochannel platform for the detection of hydrogen peroxide, H(2)O(2)…
The trees in the middle of Paradise (Gn 2:9) during the Great Lent: Orthodox hymnography as biblical interpretation
2022
The article examines the interpretation of the Scripture in Byzantine hymnography during the Great Lent. Some notable recent contributions focus on Andrew of Crete’s and Romanos the Melodist’s compositions, illustrating the hymnographic way of understanding the Scriptures. The author of this study presents a selection of stanzas from hymns of the Triodion that refer to the trees of Paradise. Hymnography perceives the trees in Genesis 2–3 in direct connection with the cross. Only rarely is the tree of life a metaphor for Jesus, as the shadow of the tree of the cross is seldom a metaphor for protection. Another interesting aspect in relation to hymnography is the fact that it represents a typ…
Chromogranin A in the Mammalian Merkel Cell: Cellular and Subcellular Distribution
1989
Chromogranin-A (CGA), which accounts for more than half the soluble matrix protein in secretory granules of various neuroendocrine cells, has a wide spectrum of potential biological roles and is considered an important marker of the diffuse neuroendocrine system (DNES). Light and electron microscopic immunohistochemistry of mammalian skin revealed that Merkel cells are exclusively CGA-immunoreactive (ir) and that the immunoreaction is localized in the secretory granules. This finding supports the classification of the Merkel cell as a member of the DNES. The CGA immunoreactivity was restricted to Merkel cells of pigs and humans. In human embryonic skin, CGA was expressed in Merkel cells as …
Dabisku mikroorganismu asociāciju izmantošana bioūdeņraža iegūšanai no glicerīna
2017
Ražojot ūdeņradi no jēlglicerīna anaerobajā fermentācijā, iegūst atjaunojamu enerģijas nesēju, kā arī lietderīgi izmanto atkritumproduktu – jēlglicerīnu. Šajā darbā pētīta dabisku mikroorganismu asociāciju – ezera dūņu spēja izmantot jēlglicerīnu ūdeņraţa producēšanai, izvērtēta 20°C un 37°C temperatūras ietekme, kā arī salīdzināta jēlglicerīna un analītiskā glicerīna ietekme uz ūdeņraţa iznākumu. Ar masspektrometriju noteikts gāzu sastāvs. Izmantojot mikrobioloģiskās analīzes, noteikta populāciju dinamika un identificētas dominējošās sugas. Novērtēta organisko vielu koncentrācija un sastāvs ar šķidruma hromatogrāfiju un ar ķīmiskā skābekļa patēriņa noteikšanu. Noskaidrots, ka visvairāk ūde…
Effect of cytochrome P450 inhibitors (diethyl dithiocarbamate, ketoconazole and grapefruit juice) on the pharmacokinetics of all-trans-retinoic acid.
2004
Diethyl dithiocarbamate (DEDTC) has been reported to be a more powerful inhibitor of all-trans-retinoic acid (ATRA) in vitro metabolism than the well-established cytochrome P450 (CYP) inhibitor ketoconazole (KC). In recent years grapefruit juice (GJ) has been shown to be able to increase the oral bioavailability of several drugs by inhibiting intestinal CYP. This study investigated the in vivo effect of these CYP inhibitors on the pharmacokinetics of ATRA. The latter was administered to rats as a constant-rate intravenous (i.v.) infusion (0.48 mg h(-1) kg(-1)) during 10 h and orally (1.6 mg kg(-1)). DEDTC (320 mg kg(-1) x 2 i.v., 6.4 and 32 mg kg(-1) per os (p.o.)) did not change the ATRA c…
Projections from the medial cortex in the brain of lizards: correlation of anterograde and retrograde transport of horseradish peroxidase with Timm s…
1988
Efferent projections of the medial cortex of the lizards Podarcis hispanica and Gallotia stehlinii were studied by examining the transport of horseradish peroxidase; results were correlated with those from Timm-stained sections. Two efferent systems were found. The first reaches the distal part of the outer plexiform layer in the medial, dorsomedial, and dorsal cortices, i.e., zones that are negative to Timm staining, and possibly originates from horizontal fusiform neurons. The second reaches the Timm-positive zones in the cortex and septum and is topographically arranged: the vertical portion of the intermediate and caudal medial cortex and the entire rostral medial cortex project to the …
The Lost Paradise
2018
A vast array of studies focuses on religious tourism as the promising segment of tourism growth for next decades, this is a really prominent theme concerns many scholars today. However, our chapter is on the opposite direction, tourism is an expression of religiosity enrooted in Western Culture. this chapter explores the religiosity of tourism in order to expand the current understanding we have of this complex phenomenon. Far from representing a radical critique to some scholars or some position, this chapter aims to become in a contribution to expand the current paradigms in tourism-led research.
Chelation of synaptic zinc induces overexcitation in the hilar mossy cells of the rat hippocampus.
2004
Complete removal of synaptic zinc by the chelator dietyldithiocarbamate (DEDTC; 500 mg/kg i.p.) in rat was followed by convulsive behaviour including wet dog shakes alternating immobility. Histological analysis 1 day after DEDTC administration detected expression of heat shock protein in the hippocampus restricted to hilar cells. These cells colocalize the marker for neurons and the glutamate receptor GluR2/3 showing that they are excitatory neurons. Additionally, they projected to the contralateral dentate gyrus. Therefore, they correspond to hilar mossy cells. These data show that the synaptic zinc has a role in normal hippocampus avoiding overexcitation, that would impair functionality e…