Search results for "RAM"

showing 10 items of 35643 documents

A new genus for the eastern dwarf galagos (Primates: Galagidae).

2017

13 pages; International audience; The family Galagidae (African galagos or bushbabies) comprises five genera: EuoticusGray, 1872; GalagoGeoffroy Saint-Hilaire, 1796; GalagoidesSmith, 1833; OtolemurCoquerel, 1859; and SciurocheirusGray, 1872, none of which is regarded as monotypic, but some (Euoticus and Otolemur) certainly qualify as oligotypic. We argue for the recognition of a sixth genus, if the taxonomy is to reflect galagid evolution accurately. Genetic evidence has consistently demonstrated that the taxa currently referred to the genus Galagoides are not monophyletic but form two clades (a western and an eastern clade) that do not share an exclusive common ancestor; we review 20 years…

0106 biological sciences0301 basic medicineAnthropologyBiogeographybushbabyBushbaby Biogeography Craniodental morphometrics Galagoides Paragalago Molecular phylogeny Vocalisations[SDV.BID]Life Sciences [q-bio]/BiodiversityBiology010603 evolutionary biology01 natural sciencesFramework agreement03 medical and health sciencesGenusGalagoidesEcology Evolution Behavior and Systematicsbiogeographymolecular phylogeny[ SDV.BID ] Life Sciences [q-bio]/BiodiversityvocalizationsEcologycraniodental morphometricsCentral africaParagalago030104 developmental biologyMolecular phylogeneticsAnimal Science and ZoologyGalagoidesVocalisations
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Recombinant laccase from Pediococcus acidilactici CECT 5930 with ability to degrade tyramine

2017

Biogenic amines degradation by bacterial laccases is little known, so we have cloned and heterologously expressed, in E. coli, a new laccase from Pediococcus acidilactici CECT 5930 (Lpa5930), a lactic acid bacterium commonly found in foods able to degrade tyramine. The recombinant enzyme has been characterized by physical and biochemical assays. Here we report the optimization of expression and purification procedures of this laccase. DNA encoding sequence of laccase from P. acidilactici was amplified by PCR and cloned into the expression plasmid pET28a for induction by isopropyl-β-D-thiogalactoipyranoside. Protein expression was performed in E. coli BL21(DE3) harboring pGro7 plasmid expres…

0106 biological sciences0301 basic medicineArabinoseMolecular biologylcsh:MedicineLaccasesBiochemistryBiotecnologia01 natural sciencesSubstrate Specificitylaw.inventionDatabase and Informatics Methodschemistry.chemical_compoundlawRecombinant Protein PurificationCloning MolecularAmineslcsh:Sciencechemistry.chemical_classificationMultidisciplinaryABTSbiologyOrganic CompoundsTemperatureHydrogen-Ion ConcentrationTyramineRecombinant ProteinsEnzymesChemistryRecombination-Based AssayBiochemistryPhysical SciencesRecombinant DNAElectrophoresis Polyacrylamide GelOxidation-ReductionSequence AnalysisResearch ArticleProtein PurificationBioinformaticsTyramineLibrary ScreeningDNA constructionResearch and Analysis Methods03 medical and health sciencesBacterial ProteinsSequence Motif Analysis010608 biotechnologyAmino Acid SequenceBenzothiazolesPediococcus acidilacticiLaccaseMolecular Biology Assays and Analysis TechniquesBase SequenceMolecular massLaccaseOrganic Chemistrylcsh:RChemical CompoundsBiology and Life SciencesProteinsPediococcus acidilacticiSequence Analysis DNAbiology.organism_classificationMolecular biology techniques030104 developmental biologyEnzymechemistryPlasmid ConstructionEnzymologySpectrophotometry Ultravioletlcsh:QSulfonic AcidsEnzimsProteïnesPurification TechniquesPLOS ONE
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Preliminary Study on the In vitro and In vivo Effects of Asparagopsis taxiformis Bioactive Phycoderivates on Teleosts

2016

Several compounds from marine organisms have been studied for their potential use in aquaculture. Among the red algae, Asparagopsis taxiformis is considered one of the most promising species for the production of bioactive metabolites with numerous proposed applications. Here, the in vitro antibacterial activity, the easy handling and the absence of adverse effects on marine fish species are reported. Depending on the seasonal period of sampling, ethanol extracts of A. taxiformis exhibited significantly different inhibitory activity against fish pathogenic bacteria. The extract obtained in late spring showed strong antibacterial activity against Aeromonas salmonicida subsp. salmonicida, Vib…

0106 biological sciences0301 basic medicineAsparagopsis taxiformisaquaculture.Physiologyhematological parametersRed algaemedicine.disease_cause01 natural scienceslcsh:PhysiologyMicrobiologyAsparagopsis taxiformis Dicentrarchus labrax Sparus aurataantibacterial activity hematological parameter oxidative stresstoxicity03 medical and health sciencesAquacultureantibacterial activitySparus aurataPhysiology (medical)medicineoxidative stressDicentrarchus labraxSea basshematological parameterOriginal ResearchVibrio alginolyticusImmune stimulationlcsh:QP1-981biologybusiness.industry010604 marine biology & hydrobiologytoxicityPathogenic bacteriabiology.organism_classificationAeromonas salmonicida030104 developmental biologyPhotobacterium damselaeAsparagopsis taxiformisbusinessFrontiers in Physiology
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Specimens at the Center: An Informatics Workflow and Toolkit for Specimen-level analysis of Public DNA database data

2016

Pham, Kasey K. [et al.]

0106 biological sciences0301 basic medicineBiodiversityPlant ScienceComputational biologyBiology010603 evolutionary biology01 natural sciencesSet (abstract data type)03 medical and health sciencesBotanyDNA databaseGeneticsSupermatrixEcology Evolution Behavior and SystematicsPhylogenetic treePhylogenetic workflowTaxon disparity index (TDI)030104 developmental biologyWorkflowCarexGenBankIdentity (object-oriented programming)CyperaceaeSupermatrixSpecimen-level data
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Biochemical characterization of the skeletal matrix of the massive coral, Porites australiensis - The saccharide moieties and their localization.

2018

11 pages; International audience; To construct calcium carbonate skeletons of sophisticated architecture, scleractinian corals secrete an extracellular skeletal organic matrix (SOM) from aboral ectodermal cells. The SOM, which is composed of proteins, saccharides, and lipids, performs functions critical for skeleton formation. Even though polysaccharides constitute the major component of the SOM, its contribution to coral skeleton formation is poorly understood. To this end, we analyzed the SOM of the massive colonial coral, Porites australiensis, the skeleton of which has drawn great research interest because it records environmental conditions throughout the life of the colony. The coral …

0106 biological sciences0301 basic medicineBiomineralizationGlycanCoralMatrix (biology)Polysaccharide010603 evolutionary biology01 natural sciencesCalcium Carbonate03 medical and health sciencesCalcification PhysiologicSaccharideStructural BiologyMonosaccharideAnimals14. Life underwater[SDV.IB.BIO]Life Sciences [q-bio]/Bioengineering/BiomaterialsSkeletonchemistry.chemical_classificationbiologySkeletal organic matrixLectinProteinsAnthozoaSkeleton (computer programming)Porites australiensisExtracellular Matrix030104 developmental biologyBiochemistrychemistrybiology.proteinMicroscopy Electron ScanningCoralCrystallizationBiomineralization
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The Legal International Wildlife Trade Favours Invasive Species Establishment: The Monk and Ring-Necked Parakeets in Spain

2018

The international wildlife trade is a lucrative business. Although a huge variety of animal groups are trafficked, the Psittaciformes (parrots) are amongst the most traded avian groups. Deliberate or accidental releases of imported parrots have led to the establishment of feral populations in many countries. Far from their native habitats, parrots may cause economic and ecological damage, and may even favour the transmission of zoonotic diseases. Despite this, the links between numbers of imported individuals and the establishment of non-native populations is not well known. In this study, we analysed data on imports of two well-known invasive parrots, the Monk Parakeet Myiopsitamonachus an…

0106 biological sciences0301 basic medicineCITESbiologyPsittacula krameriParakeetbiology.organism_classification010603 evolutionary biology01 natural sciencesInvasive speciesWildlife tradeFishery03 medical and health sciences030104 developmental biologyGeographyAnimal groupsHabitatbiology.animalAnimal Science and ZoologyPsittaciformesEcology Evolution Behavior and SystematicsArdeola
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A non-venomous sPLA2 of a lepidopteran insect: Its physiological functions in development and immunity

2018

Eicosanoids are oxygenated C20 polyunsaturated fatty acids that mediate various physiological processes in insects. Eicosanoid biosynthesis begins with a C20 precursor, arachidonic acid (5,8,11,14-eicosatetraenoic acid: AA). AA is usually released from phospholipids at sn-2 position by catalytic activity of phospholipase A2 (PLA2). Although various PLA2s classified into 16 gene families (= Groups) are known in various biological systems, few PLA2s are known in insects. Only two PLA2s involved in intracellular calcium independent PLA2 (iPLA2) group have been identified in lepidopteran insects with well known eicosanoid physiology. This study reports the first secretory PLA2 (sPLA2) in lepido…

0106 biological sciences0301 basic medicineCellular immunitybiologyfungiImmunologySpodopterabiology.organism_classification01 natural sciences010602 entomology03 medical and health sciencesOpen reading framechemistry.chemical_compound030104 developmental biologyPhospholipase A2BiochemistrychemistryJuvenile hormoneExiguabiology.proteinlipids (amino acids peptides and proteins)Arachidonic acidPeptide sequenceDevelopmental BiologyDevelopmental & Comparative Immunology
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Comparative Mitogenomics of Leeches (Annelida: Clitellata): Genome Conservation and Placobdella-Specific trnD Gene Duplication.

2015

Mitochondrial DNA sequences, often in combination with nuclear markers and morphological data, are frequently used to unravel the phylogenetic relationships, population dynamics and biogeographic histories of a plethora of organisms. The information provided by examining complete mitochondrial genomes also enables investigation of other evolutionary events such as gene rearrangements, gene duplication and gene loss. Despite efforts to generate information to represent most of the currently recognized groups, some taxa are underrepresented in mitochondrial genomic databases. One such group is leeches (Annelida: Hirudinea: Clitellata). Herein, we expand our knowledge concerning leech mitochon…

0106 biological sciences0301 basic medicineClitellatalcsh:MedicineBiochemistry01 natural sciencesGenomeDatabase and Informatics MethodsRNA TransferGene DuplicationGene OrderInvertebrate GenomicsGene duplicationAnnelidslcsh:SciencePhylogenyEnergy-Producing OrganellesData ManagementGeneticseducation.field_of_studyMultidisciplinaryPhylogenetic treePhylogenetic AnalysisGenomicsGenomic DatabasesMitochondriaNucleic acidsPhylogeneticsGenes MitochondrialPlacobdella parasiticaCellular Structures and OrganellesTransfer RNAResearch ArticleComputer and Information SciencesMitochondrial DNAPopulationBioenergeticsBiologyResearch and Analysis Methods010603 evolutionary biologyEvolution MolecularOpen Reading Frames03 medical and health sciencesPhylogeneticsLeechesGeneticsAnimalsEvolutionary Systematics14. Life underwaterCodonMolecular Biology TechniquesNon-coding RNAeducationMolecular BiologyTaxonomyMolecular Biology Assays and Analysis TechniquesEvolutionary Biologylcsh:ROrganismsBiology and Life SciencesComputational BiologyCell BiologyGenome Analysisbiology.organism_classificationInvertebratesBiological Databases030104 developmental biologyAnimal GenomicsGenome MitochondrialRNAlcsh:QPLoS ONE
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Spatially-induced nestedness in a neutral model of phage-bacteria networks

2017

[EN] Ecological networks, both displaying mutualistic or antagonistic interactions, seem to share common structural traits: the presence of nestedness and modularity. A variety of model approaches and hypothesis have been formulated concerning the significance and implications of these properties. In phage-bacteria bipartite infection networks, nestedness seems to be the rule in many different contexts. Modeling the coevolution of a diverse virus¿host ensemble is a difficult task, given the dimensionality and multi parametric nature of a standard continuous approximation. Here, we take a different approach, by using a neutral, toy model of host¿phage interactions on a spatial lattice. Each …

0106 biological sciences0301 basic medicineComputer sciencevirus–host interactionsVirus host interactionsBiologyBit array010603 evolutionary biology01 natural sciencesMicrobiology03 medical and health sciencesVirologyCoevolutionContinuous approximationMulti parametricToy modelEcologyNested networksEcological network030104 developmental biologyBipartite graphNestednessMatching allele dynamicsBiological systemNeutral modelResearch ArticleCurse of dimensionalityCoevolution
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Influence of an evoked pleasant consumption context on consumers’ hedonic evaluation for minimally processed cactus pear (Opuntia ficus-indica) fruit

2016

Awareness of the need to consider a consumption context when measuring the consumer's hedonic evaluation of a food product led researchers to investigate differences among responses given by consumers in different contexts. Previous studies measured the effects of evoking a consumption context, by using photographs or a written scenario, on hedonic evaluations of consumers for food products. This study investigated the influence of evoking a consumption context on hedonic evaluation of minimally processed cactus pear (Opuntia ficus-indica) fruit, using pictures and memories of pleasant personal experiences. A hedonic scale method for measuring food preferences was used. Hedonic evaluation o…

0106 biological sciences0301 basic medicineConsumption (economics)PEAR030109 nutrition & dieteticsOpuntia ficusContext (language use)Horticulture01 natural sciencesSettore AGR/03 - Arboricoltura Generale E Coltivazioni Arboree03 medical and health sciencesHorticultureGeographySettore AGR/01 - Economia Ed Estimo RuraleCactusscenario evoked context consumer hedonic testing acceptance testing context effects quality parameters010606 plant biology & botanyActa Horticulturae
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