Search results for "RAPD"
showing 10 items of 84 documents
Genetic relatedness among environmental, clinical, and diseased-eel Vibrio vulnificus isolates from different geographic regions by ribotyping and ra…
1998
ABSTRACT Genetic relationships among 132 strains of Vibrio vulnificus (clinical, environmental, and diseased-eel isolates from different geographic origins, as well as seawater and shellfish isolates from the western Mediterranean coast, including reference strains) were analyzed by random amplified polymorphic DNA (RAPD) PCR. Results were validated by ribotyping. For ribotyping, DNAs were digested with Kpn I and hybridized with an oligonucleotide probe complementary to a highly conserved sequence in the 23S rRNA gene. Random amplification of DNA was performed with M13 and T3 universal primers. The comparison between ribotyping and RAPD PCR revealed an overall agreement regarding the high l…
Pyrosequencing vs. culture-dependent approaches to analyze lactic acid bacteria associated to chicha, a traditional maize-based fermented beverage fr…
2015
The diversity of lactic acid bacteria (LAB) associated with chicha, a traditional maize-based fermented alcoholicbeverage from Northwestern Argentina, was analyzed using culture-dependent and culture-independent approaches.Samples corresponding to 10 production steps were obtained from two local producers at Maimará(chicha M) and Tumbaya (chicha T). Whereas by culture-dependent approach a few number of species(Lactobacillus plantarum and Weissella viridescens in chicha M, and Enterococcus faecium and Leuconostocmesenteroides in chicha T) were identified, a higher quantitative distribution of taxa was found in both beveragesby pyrosequencing. The relative abundance of OTUs was higher in chic…
Characterization of sourdough lactic acid bacteria based on genotypic and cell-wall protein analyses
2003
Abstract Aims: To evaluate the effectiveness of two independent methods in differentiating a large population of lactic acid bacteria (LAB) isolated from wheat flours and sourdoughs and to correlate eventual differences/similarities among strains with their geographical origin and/or process parameters. Methods and Results: One hundred fifty strains belonging to Lactobacillus spp. and Weissella spp., plus eight type strains, one for each species, and two unidentified isolates, were characterized by randomly amplified polymorphic DNA (RAPD) and SDS-PAGE of cell-wall proteins. The RAPD analysis separated the eight type strains but did not always assign all the strains of a species to the same…
Wickerhamomyces sylviae f.a., sp. nov., an ascomycetous yeast species isolated from migratory birds.
2013
In the present work, we investigated the phylogenetic position and phenotypic characteristics of eight yeast isolates collected from migratory birds on the island of Ustica, Italy. A phylogenetic analysis based on the D1/D2 region of the large-subunit rRNA gene showed that all isolates clustered as a single separate lineage within the Wickerhamomyces clade. They exhibited distinct morphological and physiological characteristics and were clearly separated from their closest relatives, Wickerhamomyces lynferdii, Wickerhamomyces anomalus and Wickerhamomyces subpelliculosus, in blastn searches. On the basis of the isolation source, physiological features and molecular strain typing carried out …
Utilisation de fragments d'ADN polymorphes amplifies au hasard (RAPD) pour la realisation d'une cartographie genetique de Petunia hybrida hort. et d'…
1993
* INRA Laboratoire de Phytopharmacie, BV 1540, 21034 Dijon cedex (FRA) Diffusion du document : INRA Laboratoire de Phytopharmacie, BV 1540, 21034 Dijon cedex (FRA) Diplôme : Dr. d'Université
A polyphasic approach in order to identify dominant lactic acid bacteria during pasta manufacturing
2010
Using a polyphasic approach, we have isolated and identified, lactic acid bacteria (LAB) in samples directly collected from an artisanal pasta-making manufactory located in Puglia (South Italy). Samples were collected during several steps of pasta processing and LAB were isolated on MRS and M17 plates. Furthermore, strains were grouped in a total of eight species by means of randomly amplified polymorphic DNA (RAPD)-polymerase chain reaction (PCR) typing and 16S rDNA sequencing. The majority of strains were identified as belonging to Pediococcus pentosaceus and Enterococcus faecium species. The remaining strains were characterized and assigned to Weissella confusa, Pediococcus acidilactici,…
Selection and long-term cultures of high-yielding Digitalis obscura plants: RAPD markers for analysis of genetic stability
1996
Fifteen wild-growing plants of Digitalis obscura collected in three differents regions were characterized according to their capacities to biosynthesize cardenolides and to proliferate in vitro. Great genotype-dependent variabilities were found in both parameters. Selected genotypes were differentiated using randomly amplified polymorphic DNA (RAPD) markers. Once an elite genotype with high-yielding properties was isolated and micropropagated during 2 years, identity of the donor plant and the regenerants was confirmed by RAPD analysis.
Paternity testing of endangered species of birds by DNA fingerprinting and random amplified polymorphic DNA fingerprinting
1994
Since 1985 DNA fingerprinting is the method of choice to solve forensic problems such as paternity testing (Jeffreys et al., 1986) or identification of immigration cases (Jeffreys et al., 1985 a, b). Its applicability became evident not only in man but also in many biological problems. Jeffreys’probes 33.15 and 33.6 or Epplen’s oligonucleotide probes were used in DNA fingerprinting of dogs and cats (Jeffreys & Morton, 1987), non-human primates (Weiss, 1989), birds (Longmire et al., 1991, Miyaki et al., 1993), fishes (Schartl et al., 1993) or even plants (Weising et al., 1991). Especially its application in paternity testing turned to account in many species of animals (Lubjuhn et al., 1991,…
RAPD differentiation between Borderea pyrenaica and B. chouardii (Dioscoreaceae), two relict endangered taxa
2001
As currently circumscribed, the genus Borderea Mieg. comprises two narrowly distributed European taxa, Borderea pyrenaica (Bub.) Mieg. and B. chouardii (Gaussen) Heslot. An accumulation of facts such as that both species share a very close overall morphology, that only a single population of Borderea chouardii is known, and that they live in contrasting environments has led some authors to suggest that B. chouardii is a mere ecotype of the more widespread B. pyrenaica. We tested this hypothesis using RAPD markers in four populations of Borderea, three of them identified as B. pyrenaica and the other one representing the only known population of B. chouardii. Eleven out of twenty assayed pri…
Population genetic study in the Balearic endemic plant speciesDigitalis minor(Scrophulariaceae) using RAPD markers
2001
Digitalis minor (Scrophulariaceae) is a cardenolide-producing plant endemic to the eastern Balearic Islands (Mallorca, Menorca, and Cabrera) that occurs in two morphologically distinct varieties: D. minor var. minor (pubescent) and D. minor var. palaui (glabrous). Levels and patterns of genetic diversity in 162 individuals from 17 D. minor populations across the entire geographic range were assessed using random amplified polymorphic DNA (RAPD) markers. Comigrating RAPD fragments tested were found to be homologous by Southern hybridization in both var. minor and var. palaui. To avoid bias in parameter estimation, analyses of population genetic structure were restricted to those RAPD bands t…