Search results for "REF"
showing 10 items of 8690 documents
Activation of astroglial phospholipase D activity by phorbol ester involves ARF and Rho proteins.
2000
Primary cultures of rat cortical astrocytes express phospholipase D (PLD) isoforms 1 and 2 as determined by RT-PCR and Western blot. Basal PLD activity was strongly (10-fold) increased by 4beta-phorbol-12beta,13alpha-dibutyrate (PDB) (EC(50): 56 nM), an effect which was inhibited by Ro 31-8220 (0.1-1 microM), an inhibitor of protein kinase C (PKC), and by brefeldin A (10-100 microg/ml), an inhibitor of ADP-ribosylating factor (ARF) activation. Pretreatment of the cultures with Clostridium difficile toxin B-10463 (0.1-1 ng/ml), which inactivates small G proteins of the Rho family, led to a breakdown of the astroglial cytoskeleton; concomitantly, PLD activation by PDB was reduced by up to 50%…
Metabotropic glutamate receptors activate phospholipase D in astrocytes through a protein kinase C-dependent and Rho-independent pathway.
2003
Metabotropic glutamate receptors (mGluRs) are G protein-coupled receptors that mediate phospholipase D (PLD) activation in brain, but the mechanism underlying this response remains unclear. Here we used primary cultures of astrocytes as a cell model to explore the mechanism that links mGluRs to PLD. Glutamate activated both phospholipase C (PLC) and PLD with equal potency and this effect was mimicked by L-cysteinesulfinic acid, a putative neurotransmitter previously shown to activate mGluRs coupled to PLD, but not PLC, in adult brain. PLD activation by glutamate was dependent on Ca(2+) mobilization and fully blocked by both protein kinase C (PKC) inhibitors and PKC down-regulation, suggesti…
RECOVERY AND RE-USE OF BUILDING MATERIALS AND COMPONENTS IN ARCHITECTURE
2018
Gli studi sul riuso degli scarti per l’architettura hanno prodotto negli ultimi anni risultati inediti e molto originali. Questo, tuttavia, resta ancora un ambito poco esplorato, più florido in determinati contesti geografici e particolari condizioni economiche; limitato a poche esperienze di sperimentazione in altri. In questi contesti, i limiti per la diffusione di sistemi costruttivi che riusano prodotti di scarto sono essenzialmente due: innanzitutto il fattore culturale, la tradizione consolidata e la difficoltà di accettare modi di costruire e vivere radicalmente alternativi a quelli conosciuti (se non limitati ad esperienze di studio, o temporanee, o comunque che non interferiscano c…
Autonomia regionale e riforme costituzionali nella transizione del sistema politico italiano
2016
A partire dalla crisi della "Prima Repubblica", dai primi anni Novanta ad oggi, il sistema politico italiano è entrato in una lunga fase di transizione che non è ancora approdata a un nuovo assetto istituzionale e costituzionale stabile. I cambiamenti, o i tentativi di cambiamento, del sistema elettorale e partitico, del sistema di governo e del sistema statale hanno prodotto alcune parziali riforme costituzionali e varie riforme elettorali, spesso tra loro contraddittorie, ispirate, di volta in volta, da maggioranze politiche contingenti e ristrette. È in questo quadro generale che il saggio colloca l'analisi dell'autonomia regionale in Italia, per comprendere i fattori che ne spiegano l’e…
The System Hotspot: politics of refusal against the economic migrants and absence of good practicesfor contrasting the gender and sexual violence suf…
2017
Il saggio evidenzia come il discorso istituzionale, il discorso pubblico dei media e, infine, le interviste di testimoni significativi descrivono tre aspetti del fenomeno dei rifugiati / richiedenti asilo che non possono essere ricondotti in un'analisi coerente di "fare Europa" e un'interpretazione unica. L '"occidentalizzazione del pensiero critico" (Latouche, 1995) è quindi probabile che diventi un vero "nazionalismo occidentale", se i discorsi dal basso degli operatori culturali e dei mediatori linguistici non intervengono in maniera massiccia. I punti di crisi (Hotspot) confermano il tentativo di esclusione di alcuni rifugiati e richiedenti asilo. Un'esclusione dal sistema di protezione…
Rywalizacja międzypartyjna i wewnątrzpartyjna w kampanii referendum europejskiego w Wielkiej Brytanii z 2016 roku
2017
Inter‑party and intra‑party rivalry in the 2016 EU referendum campaign in the United Kingdom
 The objective of this paper is an analysis of the course of the 2016 EU referendum campaign from the perspective of the political battle between the parties and between intra‑party factions. Besides the main goals of the UK’s leaving the EU or remaining within it, the stakes of the battle included the strengthening of one’s own political camp, the weakening of one’s opponents, the gaining of power within a party or, eventually, the gaining of power in the country. The article is divided into two major parts devoted to the analysis of inter‑party rivalry and intra‑party rivalry respectively. Th…
Rywalizacja międzypartyjna i wewnątrzpartyjna w kampanii referendum europejskiego w Wielkiej Brytanii z 2016 roku
2017
The objective of this paper is an analysis of the course of the 2016 EU referendum campaign from the perspective of the political battle between the parties and between intra -party factions. Besides the main goals of the UK’s leaving the EU or remaining within it, the stakes of the battle included the strengthening of one’s own political camp, the weakening of one’s opponents, the gaining of power within a party or, eventually, the gaining of power in the country. The article is divided into two major parts devoted to the analysis of inter -party rivalry and intra -party rivalry respectively. The referendum (both its campaign and results) has demolished the existing political make -up dete…
Sources d'archives publiques conservées aux Archives nationales: quelques jalons de recontextualisation critique de la réforme de l'enseignement en 1…
2011
Présentation des fonds d'archives les plus significatifs pour écrire l'histoire de la réforme des universités et de la loi Faure du 12 novembre 1968.
Sabiedrības līdzdalība Latvijas administratīvi teritoriālās reformas procesā
2020
Bakalaura darba ‘’Sabiedrības līdzdalība Latvijas administratīvi teritoriālās reformas procesā’’ mērķis ir veikt Latvijas Republikas Vides aizsardzības un reģionālās attīstības ministrijas darbības novērtējumu sabiedrības līdzdalības jomā, konkrēti izstrādājot administratīvi teritoriālo reformu (ATR). Pētījumā izmantotas kvantitatīvas un kvalitatīvas pētniecības metodes- veikta trīs galveno rīcībpolitikas dokumentu- informatīvie ziņojumi “Par sabiedriskai apspriešanai izvirzāmo administratīvi teritoriālā iedalījuma modeli” un ‘’Par administratīvi teritoriālo iedalījumu’’, kā arī likumprojekta "Administratīvo teritoriju un apdzīvoto vietu likumus" kvantitatīva un kvalitatīva satura analīze. …
Què serà de la filosofia en el segle XXI?
2004
És normal que el fet de trobar-nos no sols a les acaballes d'un segle, sinó també d'un miHenni, ens provoque reflexions escatològiques diverses; també la filosofia pot ser objecte d'aquest tipus de reflexions que poden ben bé acompanyar un estudi sobre el que ha fet (i continua fent) la filosofia a la segona meitat del segle XX.