Search results for "RICE"

Genomic instability induced by α-pinene in Chinese hamster cell line.

2012

Here, we report the effects of exposure of mammalian cells to α-pinene, a bicyclic monoterpene used in insecticides, solvents and perfumes. Morphological analysis, performed in V79-Cl3 cells exposed for 1 h to increasing concentrations (25 up to 50 μM) of α-pinene, indicated a statistically significant increase in micronucleated and multinucleated cell frequencies; apoptotic cells were seen at 40 and 50 μM. This monoterpene caused genomic instability by interfering with mitotic process; in fact, 50% of cells (versus 19% of control cells) showed irregular mitosis with multipolar or incorrectly localised spindles. Cytogenetic analysis demonstrated high-frequency hypodiploid metaphases as well…

The DNA topoisomerase II catalytic inhibitor merbarone is genotoxic and induces endoreduplication

2012

Abstract In the last years a number of reports have shown that the so-called topoisomerase II (topo II) catalytic inhibitors are able to induce DNA and chromosome damage, an unexpected result taking into account that they do not stabilize topo II-DNA cleavable complexes, a feature of topo II poisons such as etoposide and amsacrine. Merbarone inhibits the catalytic activity of topo II by blocking DNA cleavage by the enzyme. While it was first reported that merbarone does not induce genotoxic effects in mammalian cells, this has been challenged by reports showing that the topo II inhibitor induces efficiently chromosome and DNA damage, and the question as to a possible behavior as a topo II p…

Human fetal adrenal hydroxysteroid sulphotransferase: cDNA cloning, stable expression in V79 cells and functional characterisation of the expressed e…

1995

Dehydroepiandrosterone sulphate (DHEAS) is a major adrenal secretory product, particularly in the fetus where it serves as a substrate for oestrogen biosynthesis by the placenta. The enzyme in the adrenal responsible for synthesising DHEAS, hydroxysteroid sulphotransferase (HST), is therefore essential for human development. We have isolated a full-length cDNA clone, encoding human fetal adrenal HST, and constructed a stable cell line expressing it by transfection into V79 Chinese hamster lung fibroblast cells. This cDNA was essentially identical to that isolated from adult human liver, where the role of HST is less well understood. This recombinant cell line allowed determination of the su…

Nuclear ribosomal DNA (nrDNA) concerted evolution in natural and artificial hybrids of Armeria (Plumbaginaceae)

1999

Nuclear ribosomal DNA (nrDNA) internal transcribed spacer (ITS) sequences from artificial hybrids and backcrosses between Armeria villosa ssp. longiaristata and A. colorata were studied to assess the possible effects of concerted evolution in natural hybrids. F1 artificial hybrids show the expected pattern of additive polymorphisms for five of the six variable sites as estimated from direct sequences. However, homogenization of polymorphism is already observed in the F2, and is biased towards A. colorata except for one site. In backcrosses, an expected tendency towards homogenization of polymorphic sites in the direction of the recurrent parent is observed for five sites, although this does…

Distributed Image Databases: Hybrid Similarity Functions

1998

Evaluation of ex vivo effectiveness of commercial desensitizing dentifrices

2016

BACKGROUND Dentin hypersensitivity is a short, severe pain with fast onset. Therapy aims to either prevent or decrease neural transmission or physically occlude the dentinal tubules. This study aimed to evaluate the effectiveness of commercial desensitizing dentifrice by means of an ex vivo method. MATERIAL AND METHODS Samples (n=8 lower human premolars for each group) were randomly allocated into: G1- brushing with Colgate®Sensitive Pro-Relief; G2- brushing with Sensodyne®Rapid Relief; G3- brushing with Sensodyne®Repair & Protect; and G4- brushing with Colgate®Maximum Cavity Protection. The test bodies were submitted to simulated toothbrushing and dentifrices were analyzed regarding their …

La depressione perinatale. Aspetti clinici e di ricerca sulla genitorialità a rischio

2013

Merleau-Ponty's reading of Descartes : from Cartesian duality to the new ontological structure

2019

The duality between the mind and the body has been haunting a wide field of academic discussions since René Descartes’ (1596–1650) philosophy. The problem not only concerns the inexplicable relation between immaterial and material substances, but also the relation between reflective thought and the unreflected experience: there are two fields of evidence which both have their own types of clarity, but which remain obscure for each other. The lived and practical experience is opposed to the objective knowledge of the sciences. Cartesian philosophy is established on the refusal of the non-philosophical life, which, nevertheless, it cannot refuse. This work offers an investigation of Maurice M…

Merleau-Pontyn Descartes-tulkinta : kartesiolaisesta duaalisuudesta uuteen ontologiseen rakenteeseen

2019

Englanninkielisen lectio praecursorian pohjalta suomennetussa artikkelissa johdatellaan väitöskirjan peruskysymykseen yleistajuisesti ja esittelevästi. Maurice Merleau-Pontyn (1908–1961) tulkinta René Descartesin (1596–1650) filosofiasta on omaperäinen erityisesti siksi, että hän ei pyri vain muodostamaan historiallisesti korrektia käsitystä Descartesin teoksista vaan hänen tavoitteensa on ennenkaikkea näyttää mikä Descartesin muodostamissa filosofisissa kysymyksissä ja rakenteissa vaikuttaa yhä nykyfilosofiassa. Merleau-Pontyn kriittisyys suhteessa Descartesiin ei ole vain irtiotto vaan myös uudelleenajattelua. Immateriaalisen ja materiaalisen substanssin välisen erottelun sijaan keskiöön …

Determination of total mercury in nuts at ultratrace level

2014

Abstract Total mercury, at μg kg−1 level, was determined in different types of nuts (cashew nut, Brazil nuts, almond, pistachio, peanut, walnut) using a direct mercury analyser after previous sample defatting and by cold vapour atomic fluorescence spectrometry. There is not enough sensitivity in the second approach to determine Hg in previously digested samples due to the strong matrix effect. Mercury levels in 25 edible nut samples from Brazil and Spain were found in the range from 0.6 to 2.7 μg kg−1 by using the pyrolysis of sample after the extraction of the nut fat. The accuracy of the proposed method was confirmed by analysing certified reference materials of Coal Fly Ash-NIST SRM 1633…