Search results for "RICE"

Limbal stem cells as a potential source of pancreatic beta cells

2009

BACKGROUND/RATIONAL Beta cell replacement therapy represents an alternative approach to conventional insulin regimen for the management of type 1 diabetes. Stem cells theoretically represent a suitable and renewable source of surrogate beta cells. Recently, it has been demonstrated that corneal epithelium contains stem cells in the basal layer of the limbus. Our aim was to isolate and characterize limbal stem cells (LSCs) from adult corneal-limbal biopsies, and to assess their capability to differentiate into insulin-producing cells. METHODS 2-3 mm2 biopsies were obtained from limbus and central cornea of four patients undergoing surgery for ocular diseases not involving conjuctive or corne…

No association between BRAFV660E and metalloproteinase activity in anaplastic thyroid cell lines

2008

Antitumor effect of aminobisphosphonates on anaplastic thyroid carcinoma cell lines.

2009

Analytical Performance and Validation of a Bioassay for Thyroid-Blocking Antibodies

2016

A cell-based bioassay for the measurement of thyroid blocking autoantibodies (TBAb) has been recently reported. The analytical performance and validation of this bioassay is assessed and described.Chinese hamster ovary cells expressing a chimeric thyrotropin receptor were treated with bovine (b) TSH and different concentrations of an immunoglobulin G (IgG) monoclonal human TBAb (K1-70). TBAb was measured as a function of luciferase activity relative to bTSH alone and expressed as percent inhibition. Results obtained in the chimeric cell line were compared with those of a wild-type cell line. Analytical performance studies were subsequently performed with the chimeric cell line only.Immunode…

The endoscopic measurement of intravascular pressure and flow in oesophageal varices.

1988

Subcellular fractionation of tissue culture cells.

2003

Cell fractionation techniques include some of the most important and widely used analytical tools in cell and molecular biology, and are essential for the development of cell-free assays that reconstitute complicated cellular processes. In addition to simple gradient systems, this unit discusses the immuno-purification of organelles, in particular endosomes. As antigens, purification can be achieved using endogenous or ectopically expressed proteins, provided that appropriate antibodies are available. Alternatively, tagged proteins can be used, when combined with anti-tag antibodies. Now that sequencing of the genomes of several organisms has been completed, biochemical strategies, and in p…

Characterization of the cleavage site and function of resulting cleavage fragments after limited proteolysis of Clostridium difficile toxin B (TcdB) …

2005

Clostridium difficiletoxin B (TcdB) is a single-stranded protein consisting of a C-terminal domain responsible for binding to the host cell membrane, a middle part involved in internalization, and the N-terminal catalytic (toxic) part. This study shows that TcdB is processed by a single proteolytic step which cleaves TcdB10463between Leu543and Gly544and the naturally occurring variant TcdB8864between Leu544and Gly545. The cleavage occurs at neutral pH and is catalysed by a pepstatin-sensitive protease localized in the cytoplasm and on the cytoplasmic face of intracellular membranes. The smaller N-terminal cleavage products [63 121 Da (TcdB10463) and 62 761 Da (TcdB8864)] harbour the cytotox…

Subtype-specific endothelin-A and endothelin-B receptor desensitization correlates with differential receptor phosphorylation.

1998

In the rat cardiovascular system endothelin-1 (ET-1) elicits prolonged physiologic responses mediated by the ET A receptor, whereas the effects mediated by the ET B receptor are transient. The molecular mechanisms for the subtype-specific responses are not yet clear. However, post-translational modifications such as phosphorylation and palmitoylation may play an important role. In Sf9 cells overexpressing the human ET A and ET B receptors, both subtypes are palmitoylated. However, only the ET B but not the ET A receptor is phosphorylated in a ligand-dependent manner. Because phosphorylation is believed to play an important role in ligand-dependent receptor inactivation, we analyzed whether …

Polynitroxylated hemoglobin-based oxygen carrier: inhibition of free radical-induced microcirculatory dysfunction.

1999

Reactive oxygen species have been identified as key mediators of leukocyte/endothelial cell interaction under various pathological conditions and diseases such as ischemia/reperfusion injury, inflammation, and after exposure to cigarette smoke. Consequently, antioxidants have been shown to successfully prevent the sequelae of these conditions, ranging from tissue infarction to atherogenesis. In this study we investigated whether, via its established superoxide dismutase-like activity, a novel polynitroxyl hemoglobin-based oxygen carrier (PNH), could affect the stimulation of leukocyte rolling and adhesion to endothelial cells in response to cigarette smoke. Using the dorsal skin fold chambe…

Modal Analysis of Multi-Degrees-of-Freedom Systems with Singular Matrices: Analytical Dynamics Approach

2017

Complex mechanical (e.g., multibody) systems with different types of constraints are generally performed through analytical dynamics methods. In some cases, however, it is possible that the (augmented) mass and/or stiffness matrices may derive to be singular; consequently, modal analysis, which is used extensively in the classical dynamics literature, fails. In this paper, if the uniqueness condition is satisfied by the constraints, a properly modified modal analysis is elucidated into analytical dynamics leading to the evaluation of the natural frequencies in a simple and straightforward way. Under that framework, advances of both classical and analytical dynamics are taken into considerat…