Search results for "RICH"

Electrical distribution substation remote diagnosis and control system

2014

This paper presents the conception, design, set up and realization of a prototype system for telemanagement of a MV/LV substation. The prototype has been developed according to the current standards. This system allows to monitor the status of the MV/LV substation box, of the medium voltage equipment, of the transformer and of the low voltage equipment. In particular, the system acts when some specified events occur. The prototype has been realized by using a DAQ; the software that implement the telemanagement system has been developed in LabVIEW environment. The system allows a remote monitoring of the quantities of interest that is carried out using the TeamViewer software able to establi…

Die Gesetze über den Civilprocess vor den Friedensgerichten der Ostseeprovinzen /

1882

Purification of a biologically active recombinant glyceraldehyde 3-phosphate dehydrogenase fromCandida albicans

1999

We report here the purification of a functionally active recombinant glyceraldehyde 3-phosphate dehydrogenase (GAPDH) from Candida albicans. The GAPDH protein encoded by the TDH1 gene was obtained as a glutathione S-transferase fusion protein by expression in the vector pGEX-4T-3, and purified by affinity chromatography and thrombin digestion. The purified protein displays GAPDH enzymatic activity (42 micromol NADH min(-1) mg(-1)) as well as the laminin and fibronectin binding activities previously described. In addition, the recombinant GAPDH is covalently modified by NAD linkage; this modification is stimulated by nitric oxide and probably involves a sulfhydryl group (cysteine) residue si…

Development of a biosensor for copper detection in aqueous solutions using an Anemonia sulcata recombinant GFP.

2014

Fluorescent proteins from marine organisms represent potential candidates for biosensor development. In this paper, we described the isolation of a native green fluorescent protein from Anemonia sulcata and the cloning and purification of its equivalent as a recombinant protein in Escherichia coli. Furthermore, the spectroscopic behaviours of the native and recombinant GFPs were investigated as a function of Cu2+, Cd2+, Pb 2+ and Ni2+ concentration. Our results suggest the high selectivity of both proteins at copper than the other metals and, for the recombinant protein, a great sensitivity at a very low concentration (0.1-1 μM). Moreover, starting from these data, using the combination of …

Production of biologically active light chain of tetanus toxin inEscherichia coli

1993

AbstractThe activity of the light (L) chain of tetanus toxin, and of mutants constructed by site-directed mutagenesis, was studied by expression and purification of the proteins from E. coli. Wild-type recombinant L chain (pTet87) was active in the inhibition of exocytosis from cultured bovine adrenal chromaffin cells, although at a level 5–15% of that of L chain purified from tetanus toxin. L chain mutants which terminated at Leu-438 (pTet89), or which contained a Cys-to-Ser mutation at residue 439 (pTet88) were equally as active as the full-length recombinant protein. The reduced activity of pTet87 L chain correlated with C-terminal proteolysis of the protein upon purification. A tryptic …

Comparison of Disease Clusters in Two Elderly Populations Hospitalized in 2008 and 2010

2013

<b><i>Background:</i></b> As chronicity represents one of the major challenges in the healthcare of aging populations, the understanding of how chronic diseases distribute and co-occur in this part of the population is needed. <b><i>Objectives:</i></b> The aims of this study were to evaluate and compare patterns of diseases identified with cluster analysis in two samples of hospitalized elderly. <b><i>Methods:</i></b> Data were obtained from the multicenter ‘Registry Politerapie SIMI (REPOSI)' that included people aged 65 or older hospitalized in internal medicine and geriatric wards in Italy during 2008 and 2010. The s…

Oxygen regulated gene expression in facultatively anaerobic bacteria

1994

In facultatively anaerobic bacteria such as Escherichia coli, oxygen and other electron acceptors fundamentally influence catabolic and anabolic pathways. E. coli is able to grow aerobically by respiration and in the absence of O2 by anaerobic respiration with nitrate, nitrite, fumarate, dimethylsulfoxide and trimethylamine N-oxide as acceptors or by fermentation. The expression of the various catabolic pathways occurs according to a hierarchy with 3 or 4 levels. Aerobic respiration at the highest level is followed by nitrate respiration (level 2), anaerobic respiration with the other acceptors (level 3) and fermentation. In other bacteria, different regulatory cascades with other underlyin…

Evaluating the feasibility of the Italian version of the computerized interactive remediation of cognition training for schizophrenia (circuits)

2020

Background: Circuits is a computerized cognitive remediation program for individuals with schizophrenia. this study aimed to evaluate the feasibility of the italian version of circuits. MethODs: Feasibility was measured through ad-hoc questionnaires in a non-clinical sample (N.=30; target-score 70%; study 1); in patients with schizophrenia (N.=5; target-score 60%; study 2) and cr therapists (N.=3; target-score 60%; study 2). study 3 was a pilot study investigating: 1) enrolment, compliance, and retention rates; 2) satisfaction; 3) potential post-treatment and follow-up improvements in patients with schizophrenia (N.=20). these results were used to estimate sample size and feasibility for a …

Esperienza del mondo e immaginazione alle soglie dell’età moderna. Cartografia e resoconti di viaggio nei paesi di lingua tedesca tra XV e XVI secolo.

2012

The effect of forest fuel harvesting on the fungal diversity of clear-cuts

2012

Abstract The removal of logging residues and stumps from clear-cuts has become a common forestry practice. Forest fuel harvesting decreases the initially low volume of dead wood in managed forests, but the biodiversity effects are poorly known. We studied the effects of forest fuel harvesting on decomposer fungi on clear-cut Norway spruce stands in central Finland. The number of occurrences and taxa of polypores, saprotrophic agarics and pleurotoid agarics were determined on 10 forest fuel harvested and 10 control clear-cuts 4–5 years after logging. In total, we recorded 148 fungal taxa. The total number of taxa, the number of polypore occurrences, and the number of polypore species within …