Search results for "RICH"

AF/R2 adhesin and cytopathic effect as virulence traits of diarrhea-inducing Escherichia coli O103 in European rabbit

1995

Escherichia coli strains belonging to 0103:K-:H2 serovar and rhamnose negative biovars are responsible for frequent life-threatening diarrheas in weaned rabbits from national breeding units in western Europe (Blanco et al., 1994; Camguilhem and A. Milon, 1989). According to their mechanisms of pathogenesis, these strains may be considered as enteropathogenic E. coli (EPEC)-like. They adhere to intestinal brush border and to HeLa cells by mean of an adhesin called AF/R2 (Adhesive Factor/Rabbit 2) (Milon et al., 1990). They do not produce known toxins (i.e. ST, LT, SLT, CNF, CLDT) (Blanco et al., 1994; Mariani-Kurkdjian et al., 1993) and bear sequences homologous to EPEC eaeA (Leroy et al., 1…

Characterization ofBacillus thuringiensisserovarbolivia(serotype H63), a novel serovar isolated from the Bolivian high valleys

1999

The type strain Bacillus thuringiensis var. bolivia (serotype H63), isolated from the Bolivian high valleys, has been characterized at different levels. Its parasporal crystal has an unusual shape and it is composed of a protein of 155 kDa which shows two bands of 75 and 80 kDa after activation. Analysis by PCR shows the presence of cry1 genes, and amplification with specific primers gave products for cry1 E, cry1 D, cry4 A and cry4 B with sizes different to those expected. Immunoblotting tests showed positive reaction for Cry1 E, Cry3 A, Cry4 A and Cry11 A crystal proteins. The plasmid pattern revealed two large and two small plasmids. Toxicity tests were performed against 14 insects and a…

Serotyping and genotyping of encapsulated Escherichia coli K1 sepsis isolates with a monoclonal IgG anti K1 antibody and K1 gene probes

1987

Among infectious diseases caused by E. coli the capsular type K1 plays a predominant role. E. coli K1 isolates account for 80% of cases of E. coli neonatal meningitis and 30% of E. coli sepsis strains. Serotyping of K1 strains has conventionally relied upon the use of K1-specific bacteriophages or serum agar methods with polyvalent anti K1 serum. In the study present here, 187 E. coli sepsis isolates have been analysed for production of the K1 antigen using K1 phages, K1 serum agar plates and Latex agglutination and ELISA using an IgG2a anti K1 monoclonal antibody. In total, 33 sepsis isolates (about 18%) were identified as K1 positive, with three of these strains proving negative in all te…

Contributo alla conoscenza del comportamento dei setti caricati orizzontalmente

2008

Si illustrano e si discutono i risultati di una prova di carico a rottura eseguita su un setto in vera grandezza realizzato in terreni sabbiosi. Il comportamento del sistema setto-terreni è fortemente non lineare a partire da valori del carico applicato dell’ordine di 1000 kN. Il carico orizzontale limite si raggiunge per valori dello spostamento in corrispondenza della testa del setto dell’ordine dei 10 cm, ed è principalmente governato dalla resistenza flessionale della sezione trasversale del setto.

Novità introdotte dagli ultimi riferimenti normativi in materia di agro-energie

2013

Relazione tra potenziale idrico e contenuto idrico relativo in alberi adulti di arancio 'Valencia'

2012

Plant species richness, biogeographic and conservation interest of the vascular flora of the satellite islands of Sicily: patterns, driving forces an…

2013

The vascular flora has been investigated on about 60 of the approximately 100 islands and islets of the Sicilian archipelago. This paper provides detailed information on the number of exclusive, rare, or threatened vascular plants living on these islands and islets. Focusing on the 18 most-investigated islets, we evaluate the extent to which species richness, rate of endemism, number of alien plants, and number of terrestrial habitats have been influenced by 1) geographical setting, 2) geological history, 3) geo-pedological variability, 4) bioclimate, 5) number and patchiness of local plant communities, and 6) natural and human disturbance history and regime. Special attention is directed t…

Stato fisiologico delle femmine del Punteruolo rosso delle palme ottenute dal monitoraggio e dalla cattura massale

2009

Indagini sulla presenza del feromone sessuale in Phyllonorycter delitella (Duponchel)

2009

Phyllonorycter delitella (Duponchel) (Lepidoptera: Gracillariidae) è un fillominatore che è stato rinvenuto nel 2004 nelle aree boschive siciliane su Roverella (Quercus pubescens Willd.). Nel presente lavoro si riportano i risultati delle indagini condotte sul feromone sessuale di questo fitofago, sotto il profilo comportamentale, chimico e morfologico. Per analizzare le risposte comportamentali di maschi vergini di P. delitella verso gruppi di femmine conspecifiche in fase di richiamo sono stati condotti biosaggi in olfattometro a due vie. Le prove hanno evidenziato un’attrazione significativa degli individui osservati verso le femmine vergini. Partendo da questi risultati è stata effettua…

MOLECULAR ANALYSIS OF COLLETOTRICHUM SPECIES ASSOCIATED TO OLIVE IN CALABRIA.

2013

A new molecular approach based on the use of genus-specific primers targeting the internal transcribed spacer (ITS) regions of rDNA, was developed and used to study the diversity of Colletotrichum species associated with the olive canopy in the Gioia Tauro plain (Calabria, southern Italy). Representative symptomatic and symptomless samples of leaves, flowers and fruits were collected during 2012 and analyzed by extracting total DNA and amplifying the target region with the genus-specific primers. Amplicons were cloned and sequenced in order to use the ITS as a barcode gene. No Colletotrichum species were detected in the first sampling period (May 28, 2012), whereas around 15% of the analyze…