Search results for "RICH"
showing 10 items of 3360 documents
Characterization of heat-labile toxin-subunit B from Escherichia coli by liquid chromatography-electrospray ionization-mass spectrometry and matrix-a…
2012
The possibilities of characterizing the heat-labile enterotoxin (LT) of enterotoxigenic Escherichia coli (ETEC) by liquid chromatography electrospray mass spectrometry (LC/ESI-MS) and matrix-assisted laser desorption with time-of-flight mass spectrometry (MALDI-TOF-MS) were investigated. The B subunit from recombinant E. coli (expression in Pichia pastoris) can be detected by LC/ESI-MS expressed in P. pastoris and the charge envelope signals can be observed; LC/ESI-MS and MALDI-TOF-MS analysis allowed the acquisition of labile toxin subunit B (LTB) molecular weight and preliminary structural characterization of LTB toxin. MALDI-TOF analysis after reduction and alkylation of the protein evid…
Structural analysis of the lipid A isolated from Hafnia alvei 32 and PCM 1192 lipopolysaccharides[S]
2010
Hafnia alvei, a Gram-negative bacterium, is an opportunistic pathogen associated with mixed hospital infections, bacteremia, septicemia, and respiratory diseases. The majority of clinical symptoms of diseases caused by this bacterium have a lipopolysaccharide (LPS, endotoxin)-related origin. The lipid A structure affects the biological activity of endotoxins predominantly. Thus, the structure of H. alvei lipid A was analyzed for the first time. The major form, asymmetrically hexa-acylated lipid A built of beta-D-GlcpN4P-(1-->6)-alpha-D-GlcpN1P substituted with (R)-14:0(3-OH) at N-2 and O-3, 14:0(3-(R)-O-12:0) at N-2', and 14:0(3-(R)-O-14:0) at O-3', was identified by ESI-MS(n) and MALDI-tim…
Lernen ohne Lehrbuch im DaF-Unterricht : Initiierung eines Pilotprojekts in zwei mittelfinnischen Grundschulen
2012
Das Lernen des Deutschen in der Schule hat nicht nur in ganz Finnland, sondern auch in der Stadt Jyväskylä drastisch abgenommen. Um dieser Tendenz entgegenzuwirken, wurde Anfang 2010 in Jyväskylä ein nationales Pilotprojekt initiiert, das vom finnischen Zentralamt für Unterrichtswesen finanziert wurde. Zu den Zielen des Projekts gehörten die Entwicklung und Erforschung von Didaktik und Methodik im schulischen Fremdsprachenunterricht sowie des Fremdsprachenerwerbs des Deutschen. Speziell sollte der Einfluss authentischer Lehrmaterialien und authentischen Lernens auf die Motivation und Handlungsfähigkeit von A2-Lernenden untersucht werden. In Jyväskylä lernten ab Herbst 2010 in einem zweijähr…
Brief an Garlieb H. Merkel
1798
Ms. 930a, Nr. 13, Bl. 35r-[36v] August Hennings. Brief an Garlieb H. Merkel, Ploen, den 20. Dece[mber] 1798 Autora rokraksts / Autograph, vācu val. / Deutsch [3] lpp. / S. Attēlu numuri / Bildnummern: 930a-013-1, 930a-013-2, 930a-013-3 Hennings, der dänische Amtmann in Plön und Bekannte des dänischen Ministers Schimmelmann, schreibt in seiner Funktion als Herausgeber der Zeitschrift „Genius der Zeit“ an Merkel. Er kündigt an, einen Aufsatz zu drucken, in dem Merkel Partei für seinen Schriftstellerkollegen Johann(es) Falk ergreift, worum ihn dieser in einem anderen Brief gebeten hat (vgl. Ms. 930a-012). Merkels Aufsatz soll in der Februarausgabe 1799 erscheinen. Der Aufsatz sowie der hier üb…
Rotor bar pre-fault detection in the squirrel cage induction motors
2015
The paper deals with a diagnosis technique to detect and monitor incipient faults in the rotor bars of squirrel gage induction motors. The failure mode analysis is performed monitoring the motor axial vibrations. To accomplish the task, the authors present a mathematical model that allows relating the occurrence and the severity of the faults to the presence and the magnitude of some frequency components of the axial vibration spectrum. To validate the proposed approach, the results obtained by applying the mathematical model are compared with the ones obtained by experimental tests done on both healthy and faulty motors.
Germany Needs a National Stability Pact
2003
In many publications1, Horst Siebert has recommended that national fiscal policies within European Monetary Union be subjected to certain conditions in order to lay the foundation for a stable currency. Provisions to this effect have been laid down in Art. 104 of the EC Treaty and the European Stability and Growth Pact. The first “acid test”, however, has cast doubt on whether these provisions are sufficient to ensure sustainable solid public-sector finance in the member countries.
Déterminants de la viabilité financière des PME
2020
To be interested in the survival of SME (small and medium-sized enterprises) is to question their ability to create rent and therefore their financial viability. Our panel study on French SMEs, between 2000 and 2018, shows that several factors play a different role in the annuity creation process. If the effectiveness of state governance seems to play a negative role, through the policies put in place, it turns out that debt plays a positive role in the wealth creation process of SME. Given the nature of the goods or service activities, the impact of suppliers and customers is different. In any case, SME in France suffer from a problem of generation renewal and transmission of knowledge whi…
Nimrod, a Putative Phagocytosis Receptor with EGF Repeats in Drosophila Plasmatocytes
2007
SummaryThe hemocytes, the blood cells of Drosophila, participate in the humoral and cellular immune defense reactions against microbes and parasites [1–8]. The plasmatocytes, one class of hemocytes, are phagocytically active and play an important role in immunity and development by removing microorganisms as well as apoptotic cells. On the surface of circulating and sessile plasmatocytes, we have now identified a protein, Nimrod C1 (NimC1), which is involved in the phagocytosis of bacteria. Suppression of NimC1 expression in plasmatocytes inhibited the phagocytosis of Staphylococcus aureus. Conversely, overexpression of NimC1 in S2 cells stimulated the phagocytosis of both S. aureus and Esc…
Tribolium castaneum immune defense genes are differentially expressed in response to Bacillus thuringiensis toxins sharing common receptor molecules …
2015
In Tribolium castaneum larvae we have demonstrated by RNA interference knockdown that the Bacillus thuringiensis Cry3Ba toxin receptors Cadherin-like and Sodium solute symporter proteins are also functional receptors of the less active Cry3Aa toxin. Differences in susceptibility to B. thuringiensis infection might not only rely on toxin-receptor interaction but also on host defense mechanisms. We compared the expression of the immune related genes encoding Apolipophorin-III and two antimicrobial peptides, Defensin3 and Defensin2 after B. thuringiensis challenge. All three genes were up-regulated following Cry3Ba spore-crystal intoxication whereas only Defensins gene expression was induced u…
Polyester vascular prostheses coated with a cyclodextrin polymer and activated with antibiotics: Cytotoxicity and microbiological evaluation
2008
Abstract Polyester (PET) vascular grafts are used to replace or bypass damaged arteries. To minimize the risk of infection during and after surgical interventions, a PET vascular prosthesis (Polythese®) was functionalized with cyclodextrin polymers (PolyCDs) in order to obtain the controlled release of antibiotics (ABs: ciprofloxacin, vancomcyin and rifampicin). An epithelial cell line (L132) was used to determine the viability of the antibiotics, and human pulmonary microvascular endothelial cells (HPMEC) were used for cell proliferation by cell counting and cell vitality with Alamar Blue fluorescent dye. Staphylococcus aureus, Escherichia coli and Enteroccocus sp. were used to determine t…