Search results for "RICH"
showing 10 items of 3360 documents
An enhanced approach based on IEEE 1459 for the detection of disturbing loads in distorted and unbalanced three-phase power systems
2009
This paper presents an enhanced approach for the detection of disturbing loads in distorted and/or unbalanced three-phase power systems in both cases of three and four-wires. It is based on the IEEE Std. 1459-2000 effective apparent power resolution and it requires only the separation of the fundamental components of voltage and current from the remaining harmonic content. In the paper, the formulation of the novel approach is given and some simulation results are presented.
Disturbing loads identification in power systems: a single-point time-domain method based on IEEE 1459-2000
2009
This paper presents a single-point method for the identification of prevailing disturbing loads in power systems for both single-phase and three-phase applications downstream or upstream from the metering section. It is based on the simultaneous measurement of three nonactive power quantities, which are based on the IEEE Std. 1459-2000 approach. The proposed method is only based on the separation of the fundamental components from the harmonic content of voltage and current; thus, it does not require any spectral analysis of the voltages and current. In this paper, the formulation of the proposed method and its time-domain implementation are described; moreover, some simulation results are …
Comparison of two different approaches for harmonic distortion sources assessment
2021
The paper focuses the attention on the comparison of two approaches for power quality assessment in the electric grid. Both approaches are suitable for investigating the harmonic pollution source and discovering if the harmonic source comes from the customer or the utility grid. The two approaches have the same purpose but they are deeply different. The first approach is based on the knowledge of the customer and utility equivalent impedance while the second is based on single point measurement at point of common coupling PCC. In this paper, the advantages and drawbacks of the two approaches are investigated also supported by a case study.
2005
Las Tablas de Daimiel National Park is a semiarid, hypertrophic wetland located in La Mancha (Ciudad Real, Central Spain). During the period 1996-2002 we carried out monthly samplings at three sites of Las Tablas. Thirty five taxa of Euglenophytes of the genera Astasia (1 taxon), Euglena (15), Lepocinclis (6), Phacus (9), and Trachelomonas (4) were identified. Six taxa are new records for Spain. Abundance, species richness, species groups and absolute and relative biomass of Euglenophytes widely fluctuated spatio-temporally in Las Tablas, without any statistically significant relationship (P > 0.05) with organic carbon (either particulate or dissolved), which has been long considered the ma…
Museo Etnostorico delle Genti Campane
2011
Molecular characterization of an inducible p-coumaric acid decarboxylase from Lactobacillus plantarum: gene cloning, transcriptional analysis, overex…
1997
By using degenerate primers designed from the first 19 N-terminal amino acids of Lactobacillus plantarum p-coumaric acid decarboxylase (PDC), a 56-bp fragment was amplified from L. plantarum in PCRs and used as a probe for screening an L. plantarum genomic bank. Of the 2,880 clones in the genomic bank, one was isolated by colony hybridization and contained a 519-bp open reading frame (pdc gene) followed by a putative terminator structure. The pdc gene is expressed on a monocistronic transcriptional unit, which is transcribed from promoter sequences homologous to Lactococcus promoter sequences. No mRNA from pdc and no PDC activity were detected in uninduced cell extracts, indicating that the…
Transcriptional analysis of the nitrile‐degrading operon from Rhodococcus sp. ACV2 and high level production of recombinant amidase with an Escherich…
1999
Northern blotting analysis with RNA probes derived from amidase and nitrile hydratase genes from Rhodococcus sp. ACV2 revealed that both genes are part of the same operon. RNase protection mapping and sequence analysis indicated that the operon is probably under the control of a sigma 70-like promoter located upstream from the amidase gene. Plasmids were constructed with the cloned genes under tac and lac promoter control. Expression of amdA was demonstrated in Escherichia coli. In another construction, the amdA gene was inserted under the control of the bacteriophage T7 promoter. Large amounts of recombinant amidase (at least 20% of total proteins) in a soluble and active form were obtaine…
The dnaK operon of Streptomyces coelicolor encodes a novel heat-shock protein which binds to the promoter region of the operon
1995
Transcriptional studies have demonstrated that the dnaK gene of Streptomyces coelicolor A3(2) is contained within a 4.3 kb operon. The operon is transcribed from a single (transiently) heat-inducible promoter, dnaKp, that resembles the typical vegetative (sigma 70-recognized) eubacterial consensus promoter sequence. dnaK transcription was found to be heat-inducible at all stages of development in surface-grown cultures. In addition, at the normal growth temperature of 30 degrees C, dnaK transcript levels were shown to vary at different stages of development, being more abundant in young germinating cultures and in mycelium undergoing sporogenesis. The nucleotide sequence of the dnaK operon …
New insights into the regulatory mechanisms of ppGpp and DksA on Escherichia coli RNA polymerase-promoter complex
2015
The stringent response modulators, guanosine tetraphosphate (ppGpp) and protein DksA, bind RNA polymerase (RNAP) and regulate gene expression to adapt bacteria to different environmental conditions. Here, we use Atomic Force Microscopy and in vitro transcription assays to study the effects of these modulators on the conformation and stability of the open promoter complex (RPo) formed at the rrnA P1, rrnB P1, its discriminator (dis) variant and lambda pR promoters. In the absence of modulators, RPo formed at these promoters show different extents of DNA wrapping which correlate with the position of UP elements. Addition of the modulators affects both DNA wrapping and RPo stability in a promo…
Transcription in bacteriophage f1-infected Escherichia coli: Very large RNA species are synthesized on the phage DNA
1983
Fractionation of pulse-labeled RNA extracted from E. coli cells infected with phage f1 and hybridization of this RNA to f1 DNA reveals that very large species are synthesized on the phage genome. Hybridization of the RNA to specific fragments of f1 DNA shows that, in the infected cell, at least one mRNA is present into which the sequences of genes III, VI, and I are all transcribed together. This result fully explains the polar effect shown by gene III mutants on the expression of genes VI and I (Pratt et al. 1966).