Search results for "RICH"
showing 10 items of 3360 documents
Nitric oxide and sensory afferent neurones modulate the protective effects of low-dose endotoxin on rat gastric mucosal damage
1995
Pretreatment (1 h) with low doses (5-40 micrograms/kg i.p.) of Escherichia coli endotoxin dose dependently reduced the gastric mucosal damage induced by a 10 min challenge with 1 ml ethanol (50% and 100%) in conscious rats. Treatment with the nitric oxide synthesis inhibitor, NG-nitro-L-arginine methyl ester (L-NAME, 5 and 10 mg/kg i.p.), significantly inhibited the protective effects of endotoxin (40 micrograms/kg i.p.). The actions of L-NAME were reversed by the prior administration of L-arginine (100 mg/kg i.p.). The protective effects of endotoxin were not influenced by pretreatment with dexamethasone (5 mg/kg s.c. twice) or indomethacin (5 mg/kg s.c.). However, ablation of sensory affe…
Regulation of cytochrome P450 IID by acute phase mediators in C3H/HeJ mice.
1992
Abstract Cytochrome P450 IID6 is a drug metabolizing enzyme and the major target antigen in LKM-1 antibody positive chronic active hepatitis. The histological hallmark of chronic active hepatitis is a lymphocytic infiltrate in the liver. It is unknown whether and how cytokines produced and secreted by these tissue infiltrating mononuclear cells regulate the cellular expression of cytochrome P450 IID6. To study the effect of interleukin 1, tumor necrosis factor and interleukin 6 on the hepatocellular RNA expression of cytochrome P450 IID, we injected each of the cytokines in C3H HeJ mice. We found a time-dependent suppression of the cytochrome in the liver. Six hours after the intraperitonea…
A single mutation in the recombinant light chain of tetanus toxin abolishes its proteolytic activity and removes the toxicity seen after reconstituti…
1994
Specific proteolysis by the tetanus toxin light chain of a vesicle-associated membrane protein (VAMP) involved in exocytosis is thought to underlie its intracellular blockade of neurotransmitter release. To substantiate this mechanism, recombinant light chain was expressed as a maltose binding protein-light chain fusion product in Escherichia coli. After purification of affinity chromatography and cleavage with factor Xa, the resultant light chain was isolated and its identity confirmed by Western blotting and N-terminal sequencing. It exhibited activity similar to that of the native light chain in proteolyzing its target in isolated bovine small synaptic vesicles and in hydrolyzing a 62-re…
Production and characterization of the recombinant Sphingomonas chlorophenolica pentachlorophenol 4-monooxygenase.
2001
Abstract Pentachlorophenol 4-monooxygenase (PCP4MO) from Sphingomonas chlorophenolica is a flavoprotein that hydroxylates PCP in the presence of NADPH and oxygen. In order to investigate the structure and function of active site, recombinant PCP4MO (rePCP4MO) was produced in Escherichia coli as a glutathione S-transferase (GST) fusion protein. Moreover, a tobacco etch virus (TEV) protease cleavage site (EKLYFQG) was introduced into GST-PCP4MO and a his-tagged TEV protease was employed. Hence, a two-step purification protocol was developed which allowed obtaining 15–20 mg of rePCP4MO from 1 L culture. The rePCP4MO revealed identity with native enzyme by SDS–PAGE and N-terminal sequence analy…
Il cibo per via. Paesaggi alimentari in Sicilia
2011
Il contributo di S. Bonanzinga esamina quel particolare aspetto del “cibo per via” che investe l’uso della voce da parte dei venditori. Le formule cadenzate che invitano all’acquisto degli alimenti non solo confermano il ruolo centrale degli uomini come gestori della dimensione pubblica, in opposizione a quello privato delle donne, ma perpetuano anche l’arcaica connessione fra suono e cibo come espressioni dell’energia naturale. Un potere della voce di modellare il reale che implode quello degli alimenti, messi in scena entro quelle cornici sonore di antica e più recente fattura che i siciliani chiamiano abbanniati, vanniati, bbanniati o bbanniatini. Le grida di réclame continuano a proporr…
Formation of microbial organic carbonates during the Late Jurassic from the Northern Tethys (Amu Darya Basin, Uzbekistan): implications for Jurassic …
2020
23 pages; International audience; The Late Jurassic was a period of major global carbon cycle perturbations with episodes of anoxia leading to regional accumulation of organic matter in sediments worldwide. The Tubiegatan section (SW Gissar Mountains, Uzbekistan) located in the Northern Tethys, shows atypical organic-rich limestone and marl deposits (up to 6% of total organic carbon) marked by pronounced negative excursions of δ13Ccarb (amplitude of ca. 12‰) and δ13Corg (amplitude of ca. 4‰) recorded during the Middle Oxfordian (Transversarium Zone). A transdisciplinary approach including sedimentology, palynofacies characterization, mineralogy, organic and inorganic geochemistry was carrie…
DEVELOPMENT AND CHARACTERIZATION OF ADVANCED METERING AND ICT SOLUTIONS FOR SMART ENERGY DISTRICTS
2022
Climate change will have an influence on the world's 8 billion inhabitants, the majority of whom live in cities, which account for roughly two-thirds of the CO2 emissions that are at the root of the climate crisis. To attain a net-zero carbon future, a rapid transition across business models and policy is required. At the same time, policy and legislation are struggling to keep up with smart technology and the Internet of Things. The management of smart urban infrastructure is the key to successful decarbonisation and the achievement of sustainable cities. In this framework, the smart electricity infrastructure shall be equipped with integrated technologies, such solar panels, storage facil…
IL SOFTWARE IRRILAB PER GLI IMPIANTI MICROIRRIGUI: VERIFICHE NUMERICHE E SPERIMENTALI
2018
Nell’ambito delle attività di ricerca condotte presso il Dipartimento di Scienze Agrarie, Alimentari e Forestali dell’Università di Palermo è stato di recente messo a punto un nuovo metodo di calcolo ottimale delle ali gocciolanti, usate nella mi-croirrigazione, attualmente patent pending (“Metodo speditivo per il dimensiona-mento di ali gocciolanti in pendenza”, Università deli Studi di Palermo, Ministero dello Sviluppo Economico - Ufficio Italiano Brevetti e Marchi). Il metodo è stato successivamente esteso ai settori microirrigui, di forma rettangolare e comunque inclinati, in un software applicativo, denominato IrriLab. L’obiettivo del lavoro è quello di “testare” il funzionamento del s…
PARTICELLE MICROMETRICHE, LORO METODO DI PREPARAZIONE E LORO USI
2019
La presente invenzione si riferisce a particelle micrometriche comprendenti il decanoato di cetile, al loro metodo di preparazione e a loro usi. In particolare, le particelle dell’invenzione sono utili per favorire l’assorbimento transpiteliale di principi attivi scarsamente solubili e/o permeabili.
Prefazione a Migrazioni. Diritto e Società
2018
La prefazione introduce i temi trattati nel volume curato da alcuni studiosi del Dipartimento di Scienze Politiche dell'Università degli Studi di Palermo, di cui l'autore è Direttore.