Search results for "RNAs"
showing 10 items of 710 documents
A ceRNA approach may unveil unexpected contributors to deletion syndromes, the model of 5q- syndrome.
2015
In genomic deletions, gene haploinsufficiency might directly configure a specific disease phenotype. Nevertheless, in some cases no functional association can be identified between haploinsufficient genes and the deletion-associated phenotype. Transcripts can act as microRNA sponges. The reduction of transcripts from the hemizygous region may increase the availability of specific microRNAs, which in turn may exert in-trans regulation of target genes outside the deleted region, eventually contributing to the phenotype. Here we prospect a competing endogenous RNA (ceRNA) approach for the identification of candidate genes target of epigenetic regulation in deletion syndromes. As a model, we an…
MicroRNAs and cellular phenotypy.
2010
This Essay explores the notion that specialized cells have unique vulnerabilities to environmental contingencies that microRNAs help to counteract. Given the ease with which new microRNAs evolve, they may serve as ideal facilitators for the emergence of new cell types.
Murine Cytomegalovirus Major Immediate-Early Enhancer Region Operating as a Genetic Switch in Bidirectional Gene Pair Transcription
2007
ABSTRACT Enhancers are defined as DNA elements that increase transcription when placed in any orientation relative to a promoter. The major immediate-early (MIE) enhancer region of murine cytomegalovirus is flanked by transcription units ie1/3 and ie2 , which are transcribed in opposite directions. We have addressed the fundamental mechanistic question of whether the enhancer synchronizes transcription of the bidirectional gene pair (synchronizer model) or whether it operates as a genetic switch, enhancing transcription of either gene in a stochastic alternation (switch model). Clonal analysis of cytokine-triggered, transcription factor-mediated MIE gene expression from latent viral genomes…
A new method for the mapping of 5' ends of RNAs.
2008
In this article, we describe a new procedure to map 5' ends of RNAs. The procedure consists in the use of specific RNase H digestion of a hybrid formed by the RNA and a complementary DNA oligonucleotide. Northern blot hybridization of the resulting RNA fragment allows an accurate measurement of its length. Although we generally use this procedure as a control of previously performed primer extension analyses, the absence of nonspecific bands, which often occur in primer extensions on RNA templates with extended secondary structures, suggests that our method may be preferable when these difficult templates are analyzed.
Intra-allelic variation in introns of the S13-RNase allele distinguishes sweet, wild and sour cherries
2010
The cherry (Prunus avium), a self-incompatible diploid species, and the sour cherry (Prunus cerasus), a self-incompatible or self-compatible allotetraploid species derived from P. avium and Prunus fruticosa, share several S-RNase alleles, including S13. An inactive form, S13° ,i s found in some sour cherries. Two (AT) microsatellites are associated with allele S13-RNase, one in the first intron and one in the second. Their length polymorphisms were studied in 14 sweet and 17 wild cherries (both P. avium) and in 42 sour cherries. Fluorescent primers amplifying each microsatellite were designed and amplification prod- ucts sized on an automated sequencer. Variants ranged from 247 to 273 bp fo…
Molecular and physiological consequences of faulty eukaryotic ribonucleotide excision repair
2019
Abstract The duplication of the eukaryotic genome is an intricate process that has to be tightly safe‐guarded. One of the most frequently occurring errors during DNA synthesis is the mis‐insertion of a ribonucleotide instead of a deoxyribonucleotide. Ribonucleotide excision repair (RER) is initiated by RNase H2 and results in error‐free removal of such mis‐incorporated ribonucleotides. If left unrepaired, DNA‐embedded ribonucleotides result in a variety of alterations within chromosomal DNA, which ultimately lead to genome instability. Here, we review how genomic ribonucleotides lead to chromosomal aberrations and discuss how the tight regulation of RER timing may be important for preventin…
Estimación de la fAPAR sobre la Península Ibérica a partir de la inversión del modelo de transferencia radiativa 4SAIL2
2014
El objetivo de este trabajo consiste en la estimación de la fAPAR en la Península Ibérica a partir de datos MODIS. En primer lugar, se ha simulado un conjunto de datos de reflectividades y de fAPAR a partir de los modelos de transferencia radiativa de hoja (PROSPECT) y de cubiertas heterogéneas (4SAIL2). En segundo lugar, se ha entrenado un conjunto de redes neuronales artificiales (RNAs) para obtener mediante inversión la relación entre la fAPAR y las reflectividades simuladas y así calcular, por último, la fAPAR de la Península Ibérica a partir de imágenes de reflectividad de MODIS. Además, se ha analizado la influencia de la configuración de observación e iluminación, nadir y oblicua. La…
Analysis of S-allele genetic diversity in Sicilian almond germplasm comparing different molecular methods
2015
Italian almond germplasm is characterized by a wide diversity in several growing areas among which Sicily is one of the most important. Analysis with consensus and specific primers and DNA sequencing was performed to investigate S-RNase genetic diversity and to elucidate the homology rate within a genetic pool of 27 Italian accessions. Interestingly, some of the self-compatible cultivars did not show the presence of Sf allele. Amplicons from consensus and allele-specific PCR primers revealed a high level of variability. Sequencing of all the S-RNase amplicons derived from consensus primers allowed the identification of two new S-RNase alleles (S51 and S52). Surprisingly, despite the AA repl…
Aging Successfully: The Role of Genetics and Environment in the Era of the Aging-Boom. Potential Therapeutic Implications
2019
Aging is one of the main health-related challenges in the world. The average life expectancy of the global population at birth is increasing up to 72 years, in 2016 and it increases about two years every decade. The healthcare costs in many countries are very high because of the increased number of unhealthy populations, and the consequent increase of severe age-related disabilities [1]. Therefore, the goal of the future should be the achievement of the so-called “health-span” (healthy-life-span), more than the treatment of age-related disease to prevent the collapse of the health system. In order to achieve this objective, it is necessary to identify new targets and biomarkers and to addre…
Iconografía de urnas cinerarias romanas en los grabados de Carlo Antonini
1991
CARLO ANTONINI, PERTENECIENTE A LA ESCUELA ROMANA, A CABALLO ENTRE LOS SIGLOS XVIII Y XIX, FUE UN DESTACADO ARQUITECTO, DIBUJANTE Y GRABADOR. EN 1821 PUBLICO UN MANUAL DE TRES VOLUMENES EN EL QUE SE PRETENDIA DAR A CONOCER LAS COLECCIONES DE VASOS ANTIGUOS DE LOS MUSEOS VATICANOS Y OTRAS GALERIAS DE ROMA. EN EL PRIMER VOLUMEN SE OCUPO DE LOS VASOS ANTIGUOS DEL MUSEO PIO-CLEMENTINO. DESCRIBE LAS PIEZAS E INCLUYE GRABADOS DE LAS MISMAS. SUS INSCRIPCIONES Y SU DECORACION PERMITEN ANALIZARLAS ICONOGRAFICAMENTE, PUDIENDO CONCLUIR QUE TODOS PRESENTAN, CON VARIANTES, TEMAS PARECIDOS RELACIONADOS CON LA INMORTALIDAD. (A.B.C./ L.A.G.)