Search results for "RODO"
showing 10 items of 520 documents
Cannabinoid CB1 receptor activation modulates spontaneous contractile activity in mouse ileal longitudinal muscle.
2007
The purpose of the present study was to examine whether cannabinoid receptor agonists influence spontaneous contractile activity of longitudinal muscle in mouse ileum in vitro. Isolated segments of mouse ileum displayed spontaneous contractions with an amplitude and frequency of about 300 mg and 30 cpm, respectively. The endocannabinoid anandamide (1-100 microM), the selective cannabinoid CB(1) receptor agonist, ACEA (0.1 microM-10 microM), but not the selective cannabinoid CB(2) receptor agonist, JWH 133 (0.1 microM-10 microM), reduced in a concentration-dependent manner the spontaneous mechanical activity. The inhibitory effect consisted in a decrease of the mean amplitude of longitudinal…
Evidence for the presence of functional protease activated receptor 4 (PAR4) in the rat colon
2004
Background and aims: Protease activated receptors (PARs) have been postulated to play a role during intestinal inflammation. The presence and role played by PAR4 in gastrointestinal functions have not been fully clarified. The aims of this study were: (i) to examine expression of PAR4 in rat proximal colon; (ii) to determine the mechanical effects induced by PAR4 activation in longitudinal muscle; and (iii) to characterise the underlying mechanisms. Methods: PAR4 expression was determined by reverse transcription-polymerase chain reaction (RT-PCR) and immunohistochemistry. Mechanical activity was recorded as changes in isometric tension. Results: A PCR product corresponding to the predicted…
Arginine vasopressin, via activation of post-junctional V1 receptors, induces contractile effects in mouse distal colon
2013
The aim of this study was to analyze whether arginine vasopressin (AVP) may be considered a modulator of intestinal motility. In this view, we evaluated, in vitro, the effects induced by exogenous administration of AVP on the contractility of mouse distal colon, the subtype(s) of receptor(s) activated and the action mechanism. Isometric recordings were performed on longitudinal and circular muscle strips of mouse distal colon. AVP (0.001 nM-100 nM) caused concentration-dependent contractile effects only on the longitudinal muscle, antagonized by the V1 receptor antagonist, V-1880. AVP-induced effect was not modified by tetrodotoxin, atropine and indomethacin. Contractile response to AVP was…
Release of [3H]Acetylcholine in Human Isolated Bronchi: Effect of Indomethacin on Muscarinic Autoinhibition
1995
Receptor-mediated regulation of acetylcholine release in the airways, particularly in humans, remains unclear. In the present study, the tissue content of acetylcholine and release of [3H]acetylcholine were measured in freshly dissected human bronchi obtained at thoracotomy. Large (main and lobar bronchi) and small (segmental and subsegmental bronchi) airways contained considerable amounts of endogenous acetylcholine (300 +/- 50 pmol/100 mg wet weight), whereas significantly less was found in lung parenchyma (60 +/- 30 pmol/100 mg). Isolated small bronchi incubated in an organ bath with the precursor [3H]choline synthesized significant amounts of [3H]acetylcholine (26,000 +/- 4,000 dpm/100 …
El enemigo principal
1981
Cinetica di assorbimento dell’acqua tramite NMR Imaging per la valutazione della efficacia di trattamenti idrorepellenti
2007
L'efficacia dei trattamenti normalmente impiegati per proteggere e/o consolidare materiali lapidei può essere valutata mediante la tecnica di imaging a risonanza magnetica nucleare (MRI), che consente di studiare in modo quantitativo, non invasivo, non distruttivo, la cinetica di assorbimento capillare dell'acqua. In campioni omogenei (Pietra di Lecce) si forma un fronte piatto (con una discontinuità netta alla scala di misura) e la tecnica MRI permette di seguire la cinetica in quanto consente di misurare, in funzione del tempo, l'altezza raggiunta da tale fronte in qualunque sezione interna del campione. Vengono confrontati i risultati ottenuti sullo stesso litotipo utilizzando due divers…
Clostridium difficile Toxins Disrupt Epithelial Barrier Function by Altering Membrane Microdomain Localization of Tight Junction Proteins
2001
ABSTRACT The anaerobic bacterium Clostridium difficile is the etiologic agent of pseudomembranous colitis. C. difficile toxins TcdA and TcdB are UDP-glucosyltransferases that monoglucosylate and thereby inactivate the Rho family of GTPases (W. P. Ciesla, Jr., and D. A. Bobak, J. Biol. Chem. 273:16021–16026, 1998). We utilized purified reference toxins of C. difficile , TcdA-10463 (TcdA) and TcdB-10463 (TcdB), and a model intestinal epithelial cell line to characterize their influence on tight-junction (TJ) organization and hence to analyze the mechanisms by which they contribute to the enhanced paracellular permeability and disease pathophysiology of pseudomembranous colitis. The increase i…
Ladri di luce. Leonardo Sciascia e Piero Guccione tra bellezza e verità
2023
The unpublished correspondence between Leonardo Sciascia and Piero Guccione from 1975 to 1989 shows the profound charge of humanity that innervates the friendship between these two great Enlightenment leaders "upside down". A "correspondence of amorous senses", despite the inevitable ideological differences, which the letters reproduced here for the first time and the original contributions of the volume give us back, under the banner of the binomial "beauty-truth".
Kryzys parlamentaryzmu a majowy zamach stanu w aspekcie bezpieczeństwa narodowego
2017
Demokracja parlamentarna nie jest typem ustroju, który może sprawnie i stabilnie funkcjonować bez solidnych podstaw ekonomicznych i społecznych, ugruntowanej i opartej na kompromisie tradycji uprawiania polityki oraz postaw obywatelskich pośród polityków i społeczeństwa. Porzucenie demokracji czyjej częściowe ograniczenie, aczkolwiek usprawiedliwione ideą racji stanu w celu wzmocnienia szeroko pojętego bezpieczeństwa narodowego, nie musi okazać się właściwym lekarstwem na problemy, z jakimi boryka się państwo. Kryzys parlamentaryzmu i przewrót majowy, zamykają okres, w którym sejm stanowił organ ostatecznie decydujący o składzie i istnieniu kolejnych rządów. Formalną zmianę we wzajemnym usy…