Search results for "RPE"
showing 10 items of 2566 documents
Antibacterial activity and cytotoxicity of selected Egyptian medicinal plants.
2011
Medicinal plants have been used as a source of remedies since ancient times in Egypt. The present study was designed to investigate the antibacterial activity and the cytotoxicity of the organic extracts from 16 selected medicinal plants of Egypt. The study was also extended to the isolation of the antiproliferative compound jaeschkeanadiol p-hydroxybenzoate (FH-25) from Ferula hermonis. The microbroth dilution was used to determine the minimal inhibitory concentration (MIC) of the samples against twelve bacterial strains belonging to four species, Providencia stuartii, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Escherichia coli, while a resazurin assay was used to assess the cytoto…
The development and management of interpersonal trust in a business network in health, exercise, and wellbeing markets
2017
The aim of this dissertation is to explore the development, management and measurement of interpersonal trust in the context of a business network. More specifically, the focus is building and managing trust in the early stages of networking by examining how interpersonal trust forms in business networks and anchors relationships, and in addition, how the level of interpersonal trust could be measured. The data for this study is a qualitative, and collected from the business network that operates in the health, exercise and wellbeing sector. This study consists of three main parts: an introductory essay that consists of the research task, conceptual framework and methodological choices, fou…
Cocleo-labirintopatie nel bambino. Come non perdere l'equilibrio.
2021
Cochleo-labyrinthopathy (CL) is an affection that involves cochlea and vestibular system. It occurs with the association of auditory symptoms (sensorineural hearing loss and tinnitus), vestibular symptoms (objective vertigo, unidirectional horizontal-rotary nystagmus and static or dynamic balance disorders) and neurovegetative signs (nausea, vomiting, pallor, sweating, etc.). The diagnosis may be difficult in paediatric age since the child is unable to clearly express their discomfort and symptoms. Multiple causes have been recognized but, in acute cases, infection is the most frequent etiological agent. The paper describes a case of CL due to Herpes Virus 1-2 infection responsive to acyclo…
Über die Verfügbarmachung des Mythos für die Symbolisierung politischer Zeitgeschichte. Ilse Langners Drama "Iphigenie und Orest"
2020
Ilse Langner's drama "Iphigenie und Orest (1977 manuscript) pivots on a historical sequence of events: war, rescue and exile, merciless revenge after the war. Why is the plot set in a Greek mythological setting? The use of myth considerably extends the frame of references, which in turn necessarily surpasses documentary correctness. The dramatist already provided a world model to the dramatist of the Atreidean myth, which focuses on basic questions of violence-stricken human existence (while Goethe formed Iphigenie as a model of humanitarianism that overcomes violence). Langner's Anti-Iphigenie shows the spiral of violence as a challenge for the post-idealist 20th century.
Soft X-ray Tomography Reveals HSV-1-Induced Remodeling of Human B Cells.
2022
Upon infection, viruses hijack the cell machinery and remodel host cell structures to utilize them for viral proliferation. Since viruses are about a thousand times smaller than their host cells, imaging virus-host interactions at high spatial resolution is like looking for a needle in a haystack. Scouting gross cellular changes with fluorescent microscopy is only possible for well-established viruses, where fluorescent tagging is developed. Soft X-ray tomography (SXT) offers 3D imaging of entire cells without the need for chemical fixation or labeling. Here, we use full-rotation SXT to visualize entire human B cells infected by the herpes simplex virus 1 (HSV-1). We have mapped the temporo…
Microtubules and microfilaments in HSV-Infected rabbit-kidney cells.
1981
In rabbit kidney cells infected with strains of Herpes simplex virus producing either cell-rounding or polycaryocytosis. Vinblastine induced paracrystals. This could be shown by phase-contrast- and electron-microscopy. Infections were done under one-step-growth conditions or at low MOI. 90 per cent noninfected cells contained stress fibers as detected by Servablue R250-staining. Shortly after recruitment into polycaryocytes, stress fibres of normal length appearing in criss-cross arrangement can be seen in the periphery of these cells. Later they polymerize to very long fibers and finally they are partially destroyed. The time of destruction depends on the MOI employed. By using Actinomycin…
Vaginal infection of mice with HSV type 2 variant ER−: A new animal model for human primary genital HSV type 2 infections
1992
Abstract Studying the pathogenesis of vaginal infections in mice with two variants of Herpes simplex virus type 2 (HSV-2) strain ER we observed that both variants ER+ and ER− caused severe vaginitis but only ER+ invaded the CNS leading to lethal neurological disease. In contrast, mice infected with ER− cleared the virus from the vagina and recovered from infection. ER+ and ER− expressed equal levels of thymidine kinase (TK) indicating a TK-independent difference in neurovirulence. Using the non-neurovirulent variant ER−, we were able to investigate humoral immune responses late after infection. Vaginal infection with ER− suppressed serum antibody formation after a secondary systemic HSV-1 i…
Mouse models of cytomegalovirus latency: overview.
2002
Abstract Background: The molecular regulation of viral latency and reactivation is a central unsolved issue in the understanding of cytomegalovirus (CMV) biology. Like human CMV (hCMV), murine CMV (mCMV) can establish a latent infection in cells of the myeloid lineage. Since mCMV genome remains present in various organs after its clearance from hematopoietic cells first in bone marrow and much later in blood, there must exist one or more widely distributed cell type(s) representing the cellular site(s) of enduring mCMV latency in host tissues. Endothelial cells and histiocytes are candidates, but the question is not yet settled. Another long debated problem appears to be solved: mCMV establ…
The effect of carvacrol on enteric viruses.
2014
Carvacrol, a monoterpenic phenol, is said to have extensive antimicrobial activity in a wide range of food spoilage or pathogenic fungi, yeast and bacteria. The aim of this study was to assess its antiviral activity on norovirus surrogates, feline calicivirus (FCV), murine norovirus (MNV), and hepatitis A virus (HAV), as well as its potential in food applications. Initially, different concentrations of carvacrol (0.25, 0.5, 1%) were individually mixed with each virus at titers of ca. 6-7 log TCID50/ml and incubated 2h at 37°C. Carvacrol at 0.5% completely inactivated the two norovirus surrogates, whereas 1% concentration was required to achieve ca. 1 log reduction of HAV. In lettuce wash wa…
Infection-induced chromatin modifications facilitate translocation of herpes simplex virus capsids to the inner nuclear membrane
2021
Herpes simplex virus capsids are assembled and packaged in the nucleus and move by diffusion through the nucleoplasm to the nuclear envelope for egress. Analyzing their motion provides conclusions not only on capsid transport but also on the properties of the nuclear environment during infection. We utilized live-cell imaging and single-particle tracking to characterize capsid motion relative to the host chromatin. The data indicate that as the chromatin was marginalized toward the nuclear envelope it presented a restrictive barrier to the capsids. However, later in infection this barrier became more permissive and the probability of capsids to enter the chromatin increased. Thus, although …