Search results for "RT-qPCR"
showing 4 items of 24 documents
Role of nitric oxide synthases from Klebsormidium nitens: structural characterization and identification of protein partners
2022
Nitric oxide (NO) is an important cellular signalling molecule regulating various physiological processes, in both animals andplants. In animals, NO synthesis is mainly catalysed by NO synthase (NOS) enzymes. During biotic or abiotic stresses, NOSlike activities that are sensitive to mammalian NOS inhibitors have been detected in plants, although no sequences encodingthe well described mammalian NOS are highlighted in land plants. Interestingly, we identified NOS-like sequences in 20 algaespecies. Among them, NOSs are found in Klebsormidium nitens the model alga to study the early transition step from aquaticalgae to land plants.As mechanisms governing NO synthesis and signalling in green l…
Detection of Parietaria Mottle Virus by RT-qPCR: An Emerging Virus Native of Mediterranean Area That Undermine Tomato and Pepper Production in Southe…
2021
Parietaria mottle virus (PMoV) is considered an emerging virus in many countries of the Mediterranean basin, especially on tomato and pepper crops. Symptoms on tomato leaves and fruits can be easily confused with those induced by cucumber mosaic virus (CMV) with necrogenic satellite RNA (CMV-satRNA), tomato spotted wilt virus (TSWV) or tomato mosaic virus (ToMV). Mixed infection of these viruses has been also reported in some tomato cultivars, with an increase in the complexity of the symptoms and severity of the disease. Although a specific serum and riboprobes have been produced, nowadays no sensitive diagnostic methods are available for the rapid PMoV detection. Here, we have developed a…
Potencial uso de la tecnología basada en microARNs para el diagnóstico y pronóstico de la escoliosis idiopática del adolescente
2021
Introducción: La escoliosis idiopática del adolescente (EIA) es una enfermedad que se caracteriza por una deformación tridimensional de la columna vertebral cuya etiología es aún desconocida. Actualmente no existe una explicación clara sobre la evolución de la enfermedad; El diagnóstico y seguimiento actual de la EIA se basa en el análisis de la curvatura de la columna mediante radiografía de rayos X, la cual está considerada como la técnica gold standard. Sin embargo, esta técnica no tiene valor pronóstico y las repetidas exposiciones han demostrado incrementar la incidencia de cáncer en la edad adulta. Es necesario, por tanto, el desarrollo de una nueva herramienta para el diagnóstico tem…