Search results for "RULE"

Integration of Spatial processing and knowledge Processing through the Semantic Web Stack

2011

International audience; This paper presents the integration process of spatial technologies and Semantic Web technologies and its associated tool. The result of this work is a spatial query and rule engine of spatial. To do so, existing ontology with spatial elements is adjusted in order to process the spatial knowledge through spatial technologies. This paper outlines the methods and the processes of these adjustments and how results are returned by our tool. The SWRL and the SPARQL language are extended for spatial purpose and the existing OWL ontology wine is used as an application example.

Pathogenicity of intrathoracically administrated Bacillus thuringiensis spores in Blatta orientalis.

2006

Abstract The ability of Bacillus thuringiensis to produce septicaemia in Periplaneta americana and Blatta orientalis has been investigated. Spores and crystals from several wild-type strains as well as spores of a B. thuringiensis crystal-deficient mutant, were first orally administrated at high doses, and no significant mortality was recorded. Intrathoracic injection of spore suspensions in P. americana revealed that this species is not very susceptible to B. thuringiensis spores. B. orientalis , by contrast, was found to be very susceptible to B. thuringiensis , with a LD 50 of about 35,000 spores, that is similar to that reported on Lepidoptera challenged with parenterally injected B. th…

OSSERVAZIONI CRITICHE A MARGINE DELLA RIFORMA DELL’AGENTE SPORTIVO. UNA LEZIONE DAGLI U.S.A.

2019

The essay, analyzing the evolution of the figure of the sports agent in the light of the recent legislative intervention by the State, shows how this activity, although never explicitly assimilated to that of a regulated profession has been, in fact, regulated as such. In this perspective, the work examines the question of compatibility with antitrust principles and highlights problems and gaps raised by the new discipline, suggesting, at the same time, a different interpretation based on the U.S. model.

Curcumin derivatives as inhibitors of Gram positive and Gram negative biofilm formation

2015

Discovery and structure-activity relationship studies of irreversible benzisothiazolinone-based inhibitors against Staphylococcus aureus sortase A tr…

2014

Gram-positive bacteria, in general, and staphylococci, in particular, are the widespread cause of nosocomial and community-acquired infections. The rapid evolvement of strains resistant to antibiotics currently in use is a serious challenge. Novel antimicrobial compounds have to be developed to fight these resistant bacteria, and sortase A, a bacterial cell wall enzyme, is a promising target for novel therapies. As a transpeptidase that covalently attaches various virulence factors to the cell surface, this enzyme plays a crucial role in the ability of bacteria to invade the host's tissues and to escape the immune response. In this study we have screened a small molecule library against rec…

Elimination of a bacterial pore-forming toxin by sequential endocytosis and exocytosis

2008

Staphylococcus aureus alpha-toxin is the archetype of bacterial pore forming toxins and a key virulence factor secreted by the majority of clinical isolates of S. aureus. Toxin monomers bind to target cells and oligomerize to form small beta-barrel pores in the plasma membrane. Many nucleated cells are able to repair a limited number of lesions by unknown, calcium-independent mechanisms. Here we show that cells can internalize alpha-toxin, that uptake is essential for cellular survival, and that pore-complexes are not proteolytically degraded, but returned to the extracellular milieu in the context of exosome-like structures, which we term toxosomes.

Staphylococcus aureus virulences faktoru un antibakteriālās rezistences noteikšana

2019

Staphylococcus aureus ir viena no izplatītākajām patogēnajām, gram pozitīvajām baktērijām, kas pateicoties tās virulences faktoriem, tādiem kā toksīniem, enzīmiem un struktūras komponentiem, spēj novājināt organisma imūno atbildi. Tādā veidā veicinot dažādu veselības traucējumu, it īpaši hospitālo infekciju un slimību attīstību. Mūsdienās, lielākoties visā pasaulē, it īpaši Dienvideiropā ir vērojama krasa MRSA izplatības palielināšanās, kas tiek nodrošināta pateicoties S. aureus sastāvā esošajam SCC mec u.c. ģenētiskajiem elementiem. Tādējādi panākot plaša spektra β-laktāma u.c. antibiotiku rezistences veidošanos, kas ar laiku var samazināt medikamentozās terapijas efektivitāti. Darba mērķi…

Phenotypic and genotypic study on antibiotic resistance and pathogenic factors of Staphylococcus aureus isolates from small ruminant mastitis milk in…

2021

Staphyloccoccus aureus is the major cause of mastitis in small ruminants in the Mediterranean farms causing severe losses to dairy industry. Antibiotic treatment has been the most common approach to control these infections. Aim of this study was to investigate antimicrobial resistance (AMR), virulence factors and biofilm-related genes of 84 Sicilian strains of S. aureus isolated from sheep and goats milk during two different periods δT1 (2006-2009) and δT2 (2013-2015). Kirby Bauer method and Polymerase Chain Reaction (PCR) were utilized to monitor AMR and related genes (mecA, tetK, tetM, ermA, ermC). Moreover, toxin genes (tsst-1, sea-see, seg-sej, and sep) and biofilm genes (bap, ica, sas…

Asymmetric Disulfanylbenzamides as Irreversible and Selective Inhibitors of Staphylococcus aureus Sortase A

2020

Abstract Staphylococcus aureus is one of the most frequent causes of nosocomial and community‐acquired infections, with drug‐resistant strains being responsible for tens of thousands of deaths per year. S. aureus sortase A inhibitors are designed to interfere with virulence determinants. We have identified disulfanylbenzamides as a new class of potent inhibitors against sortase A that act by covalent modification of the active‐site cysteine. A broad series of derivatives were synthesized to derive structure‐activity relationships (SAR). In vitro and in silico methods allowed the experimentally observed binding affinities and selectivities to be rationalized. The most active compounds were f…

A dynamic business modelling approach to design and experiment new business venture strategies

2018

Abstract Business Modelling has evolved as a key activity to reflect new business venture strategy by framing the way a firm will operate and how it will function in achieving its goals (e.g., profitability, growth, innovation, social impact). However, scholars and practitioners have criticized the adoption of a too static perspective in the design and use of conventional Business Model representations. Such a static perspective prevents nascent entrepreneurs experimenting with their Business Models and, as a result, identifying the most effective strategies, especially in terms of business sustainability and profitability. In this paper, we argue that combining conventional Business Model …