Search results for "Radford"
showing 9 items of 9 documents
Primi elementi di semiotica: La sua presenza e la sua importanza nel processo di insegnamento-apprendimento della matematica. Prefazioni di Raymond D…
2013
Efficient Extraction of Olive Pulp and Stone Proteins by using an Enzyme-Assisted Method
2014
An efficient protein extraction protocol for proteins from olive pulp and stone by using enzymes was developed. For this purpose, different parameters that affect the extraction process, such as enzyme type and content, pH, and extraction temperature and time, were tested. The influence of these factors on protein recovery was examined using the standard Bradford assay, while the extracted proteins were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). The best extraction conditions were achieved at pH 7.0 and 5% (v/v) Palatase® 20000 L (lipase) for pulp and Lecitase® Ultra (phospholipase) for stone proteins. The optimal extraction temperature and time w…
Protein Quantitation: Bradford Method
2002
Protein quantitation according to the Bradford method. Keywords: protein quantitation; bradford; coomassie brilliant blue; calorimetric quantification
La Semiótica en la Didáctica de la Matemática
2013
Available Lysine in Protein, Assay Using o-Phthalaldehyde/ N-Acetyl-L-cysteine Spectrophotometric Method
1992
An assay was based on reaction of free e-amino groups in proteins with the o-phthalaldehyde/N-acetyl-L-cysteine reagent to form isoindoles, which absorb at 335 nm. The procedure was suitable for proteins or mixtures of proteins with available lysine contents of more than 5 moles lysine/mole protein and required absence of free amino acids and peptides. This method was simpler and more convenient than other methods, since it did not require hydrolysis, amino acid analysis, long heating periods or solvent extraction.
Cannabis and Psychosis: A Causal Association
2021
Nowadays, the use of cannabis is widespread around the world and THC content of confiscated cannabis has increased in the last years. This fact has raised some consequences, especially in the mental health of the people. Although there have existed some initial controversies about the relationship between cannabis and psychosis, at the present time there are some certainties of the causal association between them. Through the Bradford Hill postulates, we will try to demonstrate that cannabis produces psychosis. The strength of the association, the consistency along the studies, biological gradient, temporality, plausibility, coherence, experimentation, and analogies support the cause-effect…
Management options for low-dose methotrexate-induced oral ulcers : a systematic review
2019
Background Oral ulcers caused by methotrexate (MTX) at low doses are a known side effect of this drug. Although increasingly more patients are medicated with MTX, these painful ulcers, without traumatic origin and resistant to any type of treatment, are not usually identified by health professionals as a side effect of the medication. Material and Methods In the absence of a consensus protocol for the effective treatment of oral lesions produced by MTX, the objective of this article was to review and analyse the information from articles related to oral ulcers produced by low-dose MTX and to record the clinical management performed and the MTX dose given to the patient. Data sources - Medli…
Let’s discuss the Criteria of Bradford Hill (1965)
2021
This letter to Editors discusses the Criteria of Bradford Hill (1965).
Use of an enzyme-assisted method to improve protein extraction from olive leaves.
2013
The improvement of protein extraction from olive leaves using an enzyme-assisted protocol has been investigated. Using a cellulase enzyme (Celluclast® 1.5L), different parameters that affect the extraction process, such as the influence and amount of organic solvent, enzyme amount, pH and extraction temperature and time, were optimised. The influence of these factors was examined using the standard Bradford assay and the extracted proteins were characterised by sodium dodecyl sulphate polyacrylamide gel electrophoresis (SDS-PAGE). The optimum extraction parameters were: 30% acetonitrile, 5% (v/v) Celluclast® 1.5L at pH 5.0 and 55°C for 15min. Under these conditions, several protein extracts…