Search results for "Real-Time"
showing 10 items of 881 documents
Development of fluorogenic probe-based PCR assays for the detection and quantification of bovine piroplasmids.
2009
This paper reports two new quantitative PCR (qPCR) assays, developed in an attempt to improve the detection of bovine piroplasmids. The first of these techniques is a duplex TaqMan assay for the simultaneous diagnosis of Babesia bovis and B. bigemina. This technique is ideal for use in South America where bovids harbour no theilerids. The second technique, which is suitable for the diagnosis of both babesiosis and theileriosis worldwide, involves fluorescence resonance energy transfer (FRET) probes. In FRET assays, Babesia bovis, B. divergens, Babesia sp. (B. major or B. bigemina), Theileria annae and Theileria sp. were all identifiable based on the melting temperatures of their amplified f…
Communication structure and collective actions in social media
2010
In this paper I present results a study of different types of social media communication and networking channels that allow for collective action (CA): Twitter, Jaiku / Qaiku, Ning and Facebook. My preliminary findings indicate, that the visual outlining and the structure of communication create different kinds of collectivity and collective actions. A status stream is effective for simple and fast repetitive mass actions and for individual mass broadcasting, while channels and threads are needed as a backchannel for more complicated, coordinated and iterative tasks and support a sense of community. When planning collective action on the Internet, ranging from citizen participation to marke…
RoadMic: Road Surface Monitoring Using Vehicular Sensor Networks with Microphones
2010
Road surface analysis including pothole reports is an important problem for road maintainers and drivers. In this paper we propose a methodology for pothole detection using mobile vehicles equipped with off the shelf microphone and global positioning devices attached to an on-board computer. The approach is generic enough to be extended for other kind of event detection using different sensors as well. The vehicles are driving on public streets and measuring pothole induced sound signals. Our approach was tested and evaluated by real world experiments in a road segment for which we had established the ground truth beforehand. The results show pothole detection with high accuracy despite the…
Low Latency Ambient Backscatter Communications with Deep Q-Learning for Beyond 5G Applications
2020
Low latency is a critical requirement of beyond 5G services. Previously, the aspect of latency has been extensively analyzed in conventional and modern wireless networks. With the rapidly growing research interest in wireless-powered ambient backscatter communications, it has become ever more important to meet the delay constraints, while maximizing the achievable data rate. Therefore, to address the issue of latency in backscatter networks, this paper provides a deep Q-learning based framework for delay constrained ambient backscatter networks. To do so, a Q-learning model for ambient backscatter scenario has been developed. In addition, an algorithm has been proposed that employ deep neur…
Culture and Real-time Polymerase Chain reaction sensitivity in the diagnosis of invasive meningococcal disease: Does culture miss less severe cases?
2019
BackgroundInvasive meningococcal disease (IMD) is a highly lethal disease. Diagnosis is commonly performed by culture or Realtime-PCR (qPCR).AimsOur aim was to evaluate, retrospectively, whether culture positivity correlates with higher bacterial load and fatal outcome. Our secondary aim was to compare culture and qPCR sensitivity.MethodsThe National Register for Molecular Surveillance was used as data source. Cycle threshold (CT), known to be inversely correlated with bacterial load, was used to compare bacterial load in different samples.ResultsThree-hundred-thirteen patients were found positive for Neisseria meningitidis by qPCR, or culture, or both; 41 died (case fatality rate 13.1%); 1…
Quantification of the Detrimental Effect of a Single Primer-Template Mismatch by Real-Time PCR Using the 16S rRNA Gene as an Example
2008
ABSTRACT We investigated the effects of internal primer-template mismatches on the efficiency of PCR amplification using the 16S rRNA gene as the model template DNA. We observed that the presence of a single mismatch in the second half of the primer extension sequence can result in an underestimation of up to 1,000-fold of the gene copy number, depending on the primer and position of the mismatch.
Robust Transmission for Reconfigurable Intelligent Surface Aided Millimeter Wave Vehicular Communications With Statistical CSI
2022
The integration of reconfigurable intelligent surface (RIS) into millimeter wave (mmWave) vehicular communications offers the possibility to unleash the potential of future proliferating vehicular applications. However, the high-mobility-induced rapidly varying channel state information (CSI) has been making it challenging to obtain the accurate instantaneous CSI (I-CSI) and to cope with the incurable high signaling overhead. The situation may become worse when the RIS with a large number of passive reflecting elements is deployed. To overcome this challenge, we investigate in this paper a robust transmission scheme for the time-varying RIS-aided mmWave vehicular communications, in which, s…
A method for modeling the battery state of charge in wireless sensor networks
2015
In this paper we propose a method for obtaining an analytic model of the battery State-of-Charge (SoC) in wireless sensor nodes. The objective is to find simple models that can be used to estimate accurately the real battery state and consequently the node lifetime. Running the model in the network nodes, we can provide the motes with the required information to implement applications that can be considered as battery-aware. The proposed methodology reduces the computational complexity of the model avoiding complicated electrochemical simulations and treating the battery as an unknown system with an output that can be predicted using simple mathematical models. At a first stage, during a se…
Sensor Node Network for Remote Moisture Measurement in Timber Based on Bluetooth Low Energy and Web-Based Monitoring System
2021
This paper proposes an IoT system based on wireless BLE connectivity to monitor the moisture content of wood, using a compact and low-cost moisture device that relies on a resistance measurement method valid for an ultra-wide range of resistance values. This device is digitally controlled with a BLE-incorporated micro-controller characterized by its small size and low power consumption, providing long-life battery. The proposed system consists of two main parts: first, the BLE moisture device including the moisture content measurement and wireless capability (BLE)
Coordination in a multi-cell multi-antenna multi-user W-CDMA system: a beamforming approach
2008
The problem of designing joint power control and optimal beamforming (JPCOB) algorithms for the downlink of a coordinated multi-cell WCDMA system is considered throughout this paper. In this case, the JPCOB design is formulated as the problem of minimizing the total transmitted power in the coordinated multi-cell system, subject to a certain quality of service requirement for each user. In this paper, the performance of two JPCOB algorithms based on different beamforming approaches is compared over the coordinated multi-cell system. The first one, obtains local beamformers by means of the well-known virtual uplink-downlink duality. In contrast, the second algorithm implements multi-base bea…