Search results for "Repo"
showing 10 items of 2753 documents
Treponema isoptericolens sp. nov., a novel spirochaete from the hindgut of the termite Incisitermes tabogae.
2008
A novel spirochaete, Treponema sp. strain SPIT5T, was isolated from hindgut contents of the drywood termite Incisitermes tabogae (Snyder). The cells of strain SPIT5T were motile, helical in shape, 0.4-0.5 microm in diameter and generally 12-20 microm long. The strain is obligately anaerobic and ferments different mono-, di- and oligosaccharides by forming ethanol as the main liquid fermentation end product. Furthermore, strain SPIT5T was able to grow anaerobically with yeast extract as sole carbon and energy source. Fastest growth was obtained at 30 degrees C, the temperature at which the termites were also grown. The optimum pH for growth was 7.2, with a range of pH 6.5-8.0. The cells poss…
Mutagenicity test system based on a reporter gene assay for short-term detection of mutagens (MutaGen assay).
2003
Abstract The construction of a bacterial mutation assay system detecting reversions of base substitutions and frameshifts in tetracycline (tet) and ampicillin resistance genes located on low copy plasmids is described. Frameshift mutations were introduced into repetitive GC-sequences and G-repeats known to be mutagenic hot-spots. Base pair substitutions were inserted in or around the active site of the ampicillinase gene thus generating reversibility of the ampicilline sensitivity. The plasmids carry genes to enable sensitive, fast and specific detection of mutagens in bacteria. MucAB was cloned into the test plasmid to enhance error-prone DNA-repair. The conventional reversion principle ha…
PCR testing for Treponema pallidum in paraffin-embedded skin biopsy specimens: test design and impact on the diagnosis of syphilis
2007
Background: Syphilis, a chronic infection caused by Treponema pallidum (T. pallidum), is a disease which is increasing in incidence, and thus more and more becoming a differential diagnosis in routine pathology. Aim: Since histological changes are not specific, we sought to develop a polymerase chain reaction (PCR)-based molecular assay for the detection of T. pallidum in formalin-fixed, paraffin-embedded tissues, and evaluate its diagnostic power, especially in comparison with other ancillary methods, i.e. immunohistochemistry and Dieterle staining. Methods: 36 skin biopsies with the clinical and /or serological diagnosis of syphilis were evaluated by morphology, immunohistochemistry and s…
LrhA as a new transcriptional key regulator of flagella, motility and chemotaxis genes in Escherichia coli
2002
The function of the LysR-type regulator LrhA of Escherichia coli was defined by comparing whole-genome mRNA profiles from wild-type E. coli and an isogenic lrhA mutant on a DNA microarray. In the lrhA mutant, a large number (48) of genes involved in flagellation, motility and chemotaxis showed relative mRNA abundances increased by factors between 3 and 80. When a representative set of five flagellar, motility and chemotaxis genes was tested in lacZ reporter gene fusions, similar factors for derepression were found in the lrhA mutant. In gel retardation experiments, the LrhA protein bound specifically to flhD and lrhA promoter DNA (apparent K-D approximate to 20 nM), whereas the promoters of…
Las repercusiones de la crisis triguera de 1629-1631 sobre las finanzas de la ciudad de Valencia
2005
La incidencia de la crisis triguera de 1629-1631 obligó a la ciudad de Valencia a desarrollar diversos sistemas de avituallamiento. Pero ello no pudo impedir que se produjera un excepcional aumento de los precios del cereal, frente al cual el Municipio desarrolló una arriesgada política que abocó en la falta de numerario de la Taula de Canvis. La búsqueda de soluciones ante el déficit se tradujo en la elaboración, desde diferentes instancias políticas, de numerosos memoriales proponiendo múltiples y variados arbitrios frente a la crisis, cuyo análisis constituye el objetivo primordial de este artículo. The effect of the wheat crisis of 1629-1631 forced the city of Valencia to develop variou…
Inventando el crimen pasional: del lenguaje de gacetilla a la prensa de sucesos (España, 1892-1920)
2021
El terme «crim passional» va sorgir a la premsa espanyola el 1892. L’objectiu d’aquest treball és desvelar el perquè i entendre’n la relació amb la premsa sensacionalista i, en especial, amb la premsa especialitzada en successos. La nostra metodologia considera que la premsa és un motor cultural en la construcció de conceptes amb incidència social. Així, doncs, explorem la presència del terme «crim passional» en un bon nombre de diaris i setmanaris per observar com es va construir un marc mental de naturalesa emocional. Es revela la incidència del concepte en l’opinió pública i en el llenguatge jurídic, així com la seva rellevància en la justificació de la violència contra les dones.
Valoda: nozīme un forma. 2. Gramatizēšanās un leksikalizēšanās latviešu valodas sistēmā
2012
Krājums „Valoda: nozīme un forma. 2. Gramatizēšanās un leksikalizēšanās latviešu valodas sistēmā” iecerēts kā 2010. un 2011. gada semināros „Gramatizēšanās un leksikalizēšanās latviešu valodas sistēmā” nolasīto referātu apkopojums.
Biases in study design, implementation, and data analysis that distort the appraisal of clinical benefit and ESMO-Magnitude of Clinical Benefit Scale…
2021
BACKGROUND: The European Society for Medical Oncology-Magnitude of Clinical Benefit Scale (ESMO-MCBS) is a validated, widely used tool developed to score the clinical benefit from cancer medicines reported in clinical trials. ESMO-MCBS scores assume valid research methodologies and quality trial implementation. Studies incorporating flawed design, implementation, or data analysis may generate outcomes that exaggerate true benefit and are not generalisable. Failure to either indicate or penalise studies with bias undermines the intention and diminishes the integrity of ESMO-MCBS scores. This review aimed to evaluate the adequacy of the ESMO-MCBS to address bias generated by flawed design, im…
Metadata to Support Data Warehouse Evolution
2009
The focus of this chapter is metadata necessary to support data warehouse evolution. We present the data warehouse framework that is able to track evolution process and adapt data warehouse schemata and data extraction, transformation, and loading (ETL) processes. We discuss the significant part of the framework, the metadata repository that stores information about the data warehouse, logical and physical schemata and their versions. We propose the physical implementation of multiversion data warehouse in a relational DBMS. For each modification of a data warehouse schema, we outline the changes that need to be made to the repository metadata and in the database.
Nationwide evaluation of day-to-day clinical pharmacists' interventions in German hospitals.
2015
tudy Objective To describe and evaluate the extent and diversity of nationwide data from clinical pharmacists’ interventions (PIs) in German hospitals. Design Retrospective analysis. Data Source The ADKA-DokuPIK German database, a national anonymous self-reported Internet-based documentation system for routine PIs as well as for medication errors reported by German hospital pharmacists. Measurements and Main Results Data sets from ADKA-DokuPIK entered between January 2009 and December 2012 were analyzed descriptively. A total of 27,610 PIs were entered, mainly by ward-based clinical pharmacists (82.5%). Most of the PIs were performed on surgical wards (37.8%), followed by anesthesiology/int…