Search results for "Reproducibility"

showing 10 items of 1976 documents

Comparison of RNA-seq and microarray-based models for clinical endpoint prediction

2015

Background Gene expression profiling is being widely applied in cancer research to identify biomarkers for clinical endpoint prediction. Since RNA-seq provides a powerful tool for transcriptome-based applications beyond the limitations of microarrays, we sought to systematically evaluate the performance of RNA-seq-based and microarray-based classifiers in this MAQC-III/SEQC study for clinical endpoint prediction using neuroblastoma as a model. Results We generate gene expression profiles from 498 primary neuroblastomas using both RNA-seq and 44 k microarrays. Characterization of the neuroblastoma transcriptome by RNA-seq reveals that more than 48,000 genes and 200,000 transcripts are being …

AdultMaleMicroarrayAdolescentEndpoint DeterminationNEUROBLASTOMA PATIENTSgenetic processesRNA-SeqBiologyBioinformaticsRISK STRATIFICATIONTranscriptomeNeuroblastomaYoung AdultREPRODUCIBILITYClinical endpointTumor Cells CulturedBREAST-CANCERHumansnatural sciencesTRANSCRIPTOMEChildGENE-EXPRESSIONOligonucleotide Array Sequence AnalysisSettore BIO/11 - BIOLOGIA MOLECOLAREEXPRESSION-BASED CLASSIFICATIONModels GeneticSequence Analysis RNAGene Expression ProfilingResearchSIGNATUREInfant NewbornBiology and Life SciencesInfantHuman genetics3. Good healthPROSTATE-CANCERGene expression profilingDIFFERENTIATIONChild PreschoolEndpoint DeterminationFemaleDNA microarray
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Development and external validation of a clinical prediction model for functional impairment after intracranial tumor surgery

2021

OBJECTIVE Decision-making for intracranial tumor surgery requires balancing the oncological benefit against the risk for resection-related impairment. Risk estimates are commonly based on subjective experience and generalized numbers from the literature, but even experienced surgeons overestimate functional outcome after surgery. Today, there is no reliable and objective way to preoperatively predict an individual patient’s risk of experiencing any functional impairment. METHODS The authors developed a prediction model for functional impairment at 3 to 6 months after microsurgical resection, defined as a decrease in Karnofsky Performance Status of ≥ 10 points. Two prospective registries in…

AdultMaleMicrosurgerymedicine.medical_specialtyFunctional impairmentAdolescentIntracranial tumorNerve manipulationoutcome predictionYoung Adult03 medical and health sciencesPostoperative Complications0302 clinical medicinePredictive Value of TestsHumansMedicineGeneralizability theoryneurosurgeryProspective StudiesRegistriesKarnofsky Performance StatusAgedRetrospective StudiesAged 80 and overBrain Neoplasmsbusiness.industryExternal validationArea under the curveReproducibility of ResultsGeneral MedicineMiddle AgedSurgerypredictive analyticsmachine learningfunctional impairment030220 oncology & carcinogenesisoncologyCohortFemaleNeurosurgerybusiness030217 neurology & neurosurgeryJournal of Neurosurgery
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Accuracy of the fetal cerebroplacental ratio for the detection of intrapartum compromise in nonsmall fetuses

2018

Objective: To study the accuracy of the cerebroplacental ratio (CPR) for the detection of intrapartum fetal compromise (IFC) in fetuses growing over the 10th centile.Methods: This was a prospective...

AdultMaleMiddle Cerebral Arterymedicine.medical_specialtyeducationGestational AgeFetal DistressUltrasonography PrenatalUmbilical Arteries03 medical and health sciences0302 clinical medicinePredictive Value of TestsPregnancymedicineFetal growthBirth WeightHumansProspective Studies030212 general & internal medicinereproductive and urinary physiologyFetus030219 obstetrics & reproductive medicineObstetricsbusiness.industryInfant NewbornPregnancy OutcomeReproducibility of ResultsObstetrics and GynecologyFetal dopplerPulsatile Flowembryonic structuresPediatrics Perinatology and Child HealthFemalebusinessThe Journal of Maternal-Fetal & Neonatal Medicine
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Determining the location of the body׳s center of mass for different groups of physically active people

2013

The purpose of the present study was to compare the location of the body center of mass (CoM) determined by using a high accuracy reaction board (RB) and two different segment parameter models for motion analysis (Dempster, 1955, DEM and de Leva, 1996 adjusted from Zatsiorsky and Seluyanov, ZAT). The body CoM (expressed as percentage of the total body height) was determined from several subjects including athletes as well as physically active students and sedentary people. Some significant differences were found in the location of the body CoM between the used segment models and the reaction board method for all male subjects (n=58, 57.03±0.79%, 56.20±0.76% and 57.60±0.76% for RB, ZAT and D…

AdultMaleModels AnatomicMovementPostureBiomedical EngineeringBiophysicsPhysical activityta3111Young AdultIce hockeyHumansOrthopedics and Sports Medicineta315SimulationMathematicsOrthodonticsbiologyAthletesRehabilitationReproducibility of ResultsTotal bodyEquipment Designbiology.organism_classificationBody HeightAthletesCalibrationFemaleCenter of massSedentary Behaviorhuman activitiesAlgorithmsJournal of Biomechanics
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Transthoracic three-dimensional echocardiographic volumetry of distorted left ventricles using rotational scanning

1997

The purpose of this study was to evaluate the relation of transthoracic three- and two-dimensional echocardiographic left ventricular volumetry to cineventriculographic volumetry. Twenty-five patients with distorted left ventricles were included in the study. To demonstrate the impact of acquiring data by rotational scanning, we performed three- and two-dimensional echocardiography in 36 latex ventricles with data acquisition in different areas of the ultrasound sectors. Interobserver and intraobserver variability were calculated to test for reproducibility. The three-dimensional imaging system consisted of a rotation motor device, a transthoracic 2.5 MHz transducer, a conventional ultrasou…

AdultMaleModels Anatomicmedicine.medical_specialtyCardiac VolumeHeart VentriclesCardiac VolumeLeft VentriclesConventional ultrasoundRoot mean squareImage Processing Computer-AssistedHumansMedicineRadiology Nuclear Medicine and imagingAgedObserver VariationReproducibilitybusiness.industryUltrasoundReproducibility of ResultsMiddle AgedTransducermedicine.anatomical_structureVentricleRegression AnalysisFemaleRadiologyCardiology and Cardiovascular MedicinebusinessEchocardiography TransesophagealJournal of the American Society of Echocardiography
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Dynamic angular three-dimensional measurement of multisegmental thoracolumbar motion in vivo.

2008

Study design Method validation and in vivo motion segment study. Objective To determine in healthy subjects in vivo intervertebral segmental kinematics and coupled motion behavior in all 3 planes simultaneously for 3 segments and to evaluate whether these results differ from those in the normal population according to the literature. Summary of background data Few studies have provided a direct invasive approach to investigate segmental kinematics in vivo. Dynamic recordings of 3-dimensional segmental motion patterns of adjacent segments have rarely been reported. To date, no studies have examined the 3-dimensional segmental movements of the thoracolumbar junction in vivo in detail. Methods…

AdultMaleMovementKinematicsMotion (physics)Thoracic VertebraeYoung AdultImaging Three-DimensionalMedicineHumansOrthopedics and Sports MedicineRange of Motion ArticularUltrasonographyReproducibilityObservational errorLumbar Vertebraebusiness.industryUltrasoundBiomechanicsReproducibility of ResultsAnatomyBiomechanical PhenomenaFemaleNeurology (clinical)Range of motionbusinessRotation (mathematics)Biomedical engineeringSpine
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Spatial variability of muscle activity during human walking: The effects of different EMG normalization approaches

2015

Human leg muscles are often activated inhomogeneously, e.g. in standing. This may also occur in complex tasks like walking. Thus, bipolar surface electromyography (sEMG) may not accurately represent whole muscle activity. This study used 64-electrode high-density sEMG (HD-sEMG) to examine spatial variability of lateral gastrocnemius (LG) muscle activity during the stance phase of walking, maximal voluntary contractions (MVCs) and maximal M-waves, and determined the effects of different normalization approaches on spatial and inter-participant variability. Plantar flexion MVC, maximal electrically elicited M-waves and walking at self-selected speed were recorded in eight healthy males aged 2…

AdultMaleNormalization (statistics)EMG normalizationmedicine.medical_specialtyWalkingElectromyographyta3112Young AdultPhysical medicine and rehabilitationmedicineHumansHuman legMuscle activityMultichannel EMGMuscle Skeletalta315MathematicsLegmedicine.diagnostic_testElectromyographyGeneral NeuroscienceReproducibility of ResultsSignal Processing Computer-AssistedElectric StimulationElectromyogramData Interpretation StatisticalGait analysisPhysical therapySpatial variabilityGait analysisPeak valuehuman activitiesLateral gastrocnemiusNEUROSCIENCE
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The Factorial Structure of the Outcome Questionnaire-45: A Study with an Italian Sample.

2008

In this article, the authors study the factorial structure of the Italian translation of the Outcome Questionnaire 45 (OQ-45) in a sample of college students (n = 522) and psychiatric outpatients (n = 301). The relative goodness of fit of six competing models of the OQ-45 was examined using confirmatory factor analysis (CFA). Specifically, the study examined the relative fit of the most frequently presented models in the literature and three alternative models. Results of the CFA provided support for the four factor bi-level solution, suggesting that the OQ-45 is a multidimensional instrument that contains one general factor and multiple unique subscale factors.

AdultMaleOQ-45 confirmatory factor analysisFactorialPsychometricsPsychometricsMental DisordersTreatment outcomeReproducibility of ResultsSample (statistics)Factor structureOutcome (probability)Confirmatory factor analysisPsychotherapyClinical PsychologyTreatment OutcomeItalyGoodness of fitSurveys and QuestionnairesStatisticsHumansFemaleFactor Analysis StatisticalStudentsPsychology
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Reliability of measuring the fat content of the lumbar vertebral marrow and paraspinal muscles using MRI mDIXON-quant sequence

2018

PURPOSE We aimed to assess the reliability of measuring the fat content of the lumbar vertebral marrow and the paraspinal muscles using magnetic resonance imaging (MRI) mDIXON-Quant sequence. METHODS Thirty-one healthy volunteers were included. All participants underwent liver mDIXON-Quant imaging on a 3.0 T Philips MRI scanner by observer A. Within two weeks, observer B repeated the scan. After the examination, each observer independently measured the fat content of the third lumbar vertebra (L3), and the psoas (PS), erector spinae (ES), and multifidus (MF) muscles on central L3 axial images. After two weeks, each observer repeated the same measurements. They were blinded to their previous…

AdultMaleObserver (quantum physics)Intraclass correlationInterclass correlationParaspinal MusclesBone Marrow CellsRisk Assessment030218 nuclear medicine & medical imaging03 medical and health sciences0302 clinical medicineLumbarBone MarrowmedicineHumansRadiology Nuclear Medicine and imagingReliability (statistics)Observer VariationReproducibilityLumbar Vertebraemedicine.diagnostic_testbusiness.industryReproducibility of ResultsMagnetic resonance imagingRepeatabilityMiddle AgedmDIXON-Quant sequenceMuscoloskeletal ImagingMagnetic Resonance ImagingAdipose TissueFemaleCardiology and Cardiovascular MedicineNuclear medicinebusiness030217 neurology & neurosurgery
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The Calgary Depression Rating Scale for Schizophrenia: development and interrater reliability of a German version (CDSS-G)

1999

A German version of the Calgary Depression Rating Scale for Schizophrenia (CDSS-G) approved by the author of the original scale is presented comprising a semi-structured interview for 9 items to sensitively and specifically assess depression in schizophrenia and related disorders. The process of translation is outlined and the finally derived CDSS-G was investigated with respect to interrater reliability in three studies. To keep comparability with the CDSS source version a standard procedure was used. Two trained raters jointly assessed ten schizophrenic patients (study I). In a second study, videotapes with the CDSS-G were presented to clinically inexperienced raters (study II, N = 14/15)…

AdultMaleObserver VariationPsychiatric Status Rating ScalesDepressive DisorderPsychometricsPsychometricsIntraclass correlationReproducibility of ResultsMiddle AgedPsychiatry and Mental healthInter-rater reliabilityRating scaleSchizophrenic PsychologySchizophreniaHumansFemaleSchizophrenic PsychologyPsychological testingPsychologyBiological PsychiatryKappaDepression (differential diagnoses)LanguageClinical psychologyJournal of Psychiatric Research
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